Artículo
Abstract:
The malignant hyperthermia (MH) is an autosomal recessive illness with incomplete penetrance which produces major economic losses in pig production. The affected animals often have a sudden death and their meats can be confiscated due to its poor quality. The halothane test has been the method to classify pigs into affected and non affected animals. This indirect test does not distinguish resistant homozygous animals from heterozygous carriers. Furthermore, it is imprecise because some pigs do not react as expected from their genotype. A point mutation (C for T) in the nucleotide 1843 of the cDNA from the ryanodine receptor gene accounts for the MH etiology. Therefore, a molecular test provides a more accurate method to determine genotypes. Thirty-five pigs from EEA-Inta Pergamino have been analysed by PCR/REA in order to discuss their genotypes for the ryr-1 gene. The results from the original halothane test and breedings were compared with those obtained from PCR/REA: we found 4 animals that did not present the expected genotype.
Registro:
| Documento: | Artículo |
| Título: | Diagnostico de la hipertermia maligna en cerdos por PCR/REA |
| Autor: | Soria, L.A.; Iglesias, G.M.; Marrube, G.; Montagna, A.E.; Huguet, M.J.; Mirande, S.L.; Flawia, M.M.; Torres, H.N. |
| Filiación: | Area de Mejoramiento Genético, Depto. de Producción Animal, Universidad de Buenos Aires, Chorroarin 280. CP.1425, Buenos Aires, Argentina Instituto de Ingeniería y Biología Molecular (INGEBI), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Vuelta de Obligado 2490. CP. 1428, Buenos Aires, Argentina |
| Año: | 1998 |
| Volumen: | 86 |
| Número: | 3-6 |
| Página de inicio: | 188 |
| Página de fin: | 192 |
| Título revista: | Anales des la Asociacion Quimica Argentina |
| ISSN: | 03650375 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03650375_v86_n3-6_p188_Soria |
Referencias:
- Mac Lennan, D., Phillips, M.S., (1992) Science, 256, p. 789
- Mac Lennan, D., Duff, C., Zorzato, F., Fujii, J., Worton, R., (1990) Nature, 343, p. 559
- Mickelson, J., Gallant, E., Litterer, L., Johnson, K., Rempel, W., Louis, C., (1988) J.Biol. Chem., 263, p. 9310
- Fujii, J., Otsu, K., Zorzato, F., De Leon, S., Khanna, V., Weiler, J., O-Brien, P., Mac Lennan, D., (1991) Nature, 253, p. 448
- Vögeli, P., Bolt, R., Fries, R., Stranzinger, G., (1994) Anim. Genet., 25, p. 59
- Pommier, S., Houde, A., (1993) J. Anim. Sci., 71, p. 420
- Coppieters, W., Van Zeveren, A., Van De Weghe, A., Peelman, L., Bouquet, Y., (1992) Vlaams Diergen. Tijds., 61, p. 68
- Rempel, W., Kandelgy, S., Kennedy, C., Irvin, L., Louis, C., (1993) J. Anim. Sci., 71, p. 1395
- Houde, A., Pommier, S., Roy, R., (1993) J. Anim. Sci., 71, p. 1414
- Zorzato, F., Fujii, J., Otsu, K., Phillips, M., Green, M., Lai, F., Meissner, G., Mac Lennan, D., (1990) J. Biol. Chem., 265, p. 2244
- Gillard, E.F., Otsu, K., Fujii, J., Duff, C., De Leon, S., Khanna, V.K., Britt, B.A., Mac Lennan, D., (1992) Genomics, 13, p. 1247
- Harbitz, I., Kristensen, T., Bosnes, M., Kran, S., Davies, W., (1992) Anim. Genet., 23, p. 395
- Miller, S.A., Dyckes, D.D., Pollesky, H.F., (1998) Nucleic Acid Res., 16, p. 1215
Citas:
---------- APA ----------
Soria, L.A., Iglesias, G.M., Marrube, G., Montagna, A.E., Huguet, M.J., Mirande, S.L., Flawia, M.M.,..., Torres, H.N. (1998) . Diagnostico de la hipertermia maligna en cerdos por PCR/REA. Anales des la Asociacion Quimica Argentina, 86(3-6), 188-192.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03650375_v86_n3-6_p188_Soria [ ]
---------- CHICAGO ----------
Soria, L.A., Iglesias, G.M., Marrube, G., Montagna, A.E., Huguet, M.J., Mirande, S.L., et al. "Diagnostico de la hipertermia maligna en cerdos por PCR/REA" . Anales des la Asociacion Quimica Argentina 86, no. 3-6 (1998) : 188-192.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03650375_v86_n3-6_p188_Soria [ ]
---------- MLA ----------
Soria, L.A., Iglesias, G.M., Marrube, G., Montagna, A.E., Huguet, M.J., Mirande, S.L., et al. "Diagnostico de la hipertermia maligna en cerdos por PCR/REA" . Anales des la Asociacion Quimica Argentina, vol. 86, no. 3-6, 1998, pp. 188-192.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03650375_v86_n3-6_p188_Soria [ ]
---------- VANCOUVER ----------
Soria, L.A., Iglesias, G.M., Marrube, G., Montagna, A.E., Huguet, M.J., Mirande, S.L., et al. Diagnostico de la hipertermia maligna en cerdos por PCR/REA. 1998;86(3-6):188-192.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03650375_v86_n3-6_p188_Soria [ ]
