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Abstract:

The cytoplasmic RNA of 10-day-old rats was studied in bulk-isolated, cortical neurons, obtained under conditions which minimize nuclear contamination. Two RNA fractions, one enriched in rRNA and the other in "polydisperse" RNA, were obtained by differential extraction with phenol. Gel electrophoresis and pulse labelling with 5-[3H]uridine were used to confirm the delayed appearance of newly synthesized rRNA in the cytoplasm and to demonstrate its stability. "Polydisperse" RNA appeared in the cytoplasm earlier and had a shorter half-life than rRNA. A wide range of molecular weights for this RNA was found with no predominant individual species. A set of cytoplasmic RNA components of molecular size between 28S and 18S was also present probably reflecting the in vivo degradation of rRNA. The significance of the unexpectedly high amounts of neuronal polydisperse RNA is discussed. © 1978 Plenum Publishing Corporation.

Registro:

Documento: Artículo
Título:Identification of polydisperse RNA in the cytoplasm of neurons isolated from the immature brain cortex
Autor:Azcurra, J.M.; Carrasco, A.E.
Filiación:Catedra Biologia Celular, Dpto, Biologia, Fac. Ciencias Exactas y Naturales, Universidad Nacional de Buenos Aires, Argentina
Catedra de Citologia e Histologia, Lab. de Neuroquimica, Fac. de Ciencias Medicas, Universidad Nacional de Buenos Aires, Argentina
Palabras clave:rna; animal experiment; brain cortex; central nervous system; nerve cell; nervous system; rat; Animal; Cerebral Cortex; Cytoplasm; Electrophoresis, Polyacrylamide Gel; Molecular Weight; Rats; RNA; RNA, Ribosomal
Año:1978
Volumen:3
Número:5
Página de inicio:521
Página de fin:534
DOI: http://dx.doi.org/10.1007/BF00963756
Título revista:Neurochemical Research
Título revista abreviado:Neurochem Res
ISSN:03643190
CODEN:NERED
CAS:RNA, 63231-63-0; RNA, 63231-63-0; RNA, Ribosomal
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03643190_v3_n5_p521_Azcurra

Referencias:

  • Hyden, H., (1967) The Neurosciences, a Study Program, pp. 248-266. , T., Melnechuck, F. O., Schmitt, The Rockefeller University Press, New York
  • Volpe, P., Giuditta, A., Biosynthesis of RNA in neurons and glial enriched fractions (1967) Brain Res., 6, pp. 228-240
  • Peterson, R.P., RNA in single identified neurons of Aplysia (1970) J. Neurochem., 17, pp. 325-328
  • Jarlstedt, J., Hamberger, A., Patterns and labelling characteristics in neuronal and glial RNA (1971) J. Neurochem., 18, pp. 921-930
  • Lovtrup-Rein, H., Grahn, B., Polysomes and polysomal RNA from nerve and glial cell fractions (1974) Brain Res., 72, pp. 123-136
  • Sellinger, O.Z., Azcurra, J.M., Bulk separation of neuronal cell bodies and glial cells in the absence of added digestive enzymes (1974) Research Methods in Neurochemistry, Vol. 2, pp. 3-38. , N., Marks, R., Rodnight, Plenum Press, New York
  • Azcurra, J.M., Sellinger, O.Z., Carrasco, A.E., In vivo labelling of cytoplasmic RNA in neurons of the immature brain cortex (1975) Brain Res., 86, pp. 144-149
  • Bishop, D.H.L., Claybrook, J.R., Spiegelman, S., Electrophoretic separation of viral nucleic acids on polyacrylamide gel (1967) J. Mol. Biol., 26, pp. 373-387
  • Azcurra, J.M., Carrasco, A.E., Caracterizacion de los acidos ribonucleicos citoplasmaticos de neuronas (1974) Rev. Soc. Arg. Biol., 50, pp. 80-87
  • Sellinger, O.Z., Azcurra, J.M., Johnson, D.E., Ohlsson, W.G., Lodin, Z., Independence of protein synthesis and drug uptake in nerve cell bodies and glial cells isolated by a new technique (1971) Nature, 230, pp. 253-256
  • Perry, R.P., Kelly, D.E., Messenger RNA-protein complexes and newly synthesized ribosomal subunits: Analysis of free particles and components of polyribosomes (1968) J. Mol. Biol., 35, pp. 37-39
  • Casola, L., Agranoff, B.W., Studies of RNA in goldfish brain. I. Isolation and in vivo labelling (1968) Brain Res., 10, pp. 227-236
  • Hadjivassiliou, A., Brawerman, G., Template and ribosomal ribonucleic acid components in the nucleus and cytoplasm of rat liver (1967) Biochemistry, 6, pp. 1934-1940
  • Campagnoni, A.T., Dutton, G.R., Mahler, H.R., Moore, W.J., Fractionation of the RNA components of rat brain polysomes (1971) J. Neurochem., 18, pp. 601-611
  • Talwar, G.P., Brain RNA (1969) The Biological Basis of Medicine, Vol. 5, pp. 77-109. , E. E., Bittar, N., Bittar, Academic Press, London
  • Perry, R.P., Kelly, D.E., Persistent synthesis of 5S RNA when production of 28S and 18S ribosomal RNA is inhibited by low doses of actinomycin D (1968) J. Cell Physiol., 72, pp. 235-245
  • Pederson, T., Proteins associated with heterogeneous nuclear RNA in eukaryotic cells (1974) J. Mol. Biol., 83, pp. 163-183
  • Cupello, A., Hyden, H., Separation of brain RNA by microelectrophoresis and agarose-acrylamide gels (1975) Neurobiology, 5, pp. 129-136
  • Coral, V.A., Wynther, P., Mathias, A.P., The effect of convulsions induced by fluorothyl on RNA synthesis in rat cerebral cortex during the recovery phase (1975) Biochem. J., 152, pp. 448-467
  • Warner, E.K., Katz, L., Penman, S., The possibility of aggregation of RNA during hot phenol SDS deproteinization (1967) Biochem. Biophys. Res. Commun., 28, pp. 152-159
  • Weil, A., Hampel, A., Preparative agarose gel electrophoresis of ribonucleic acid (1973) Biochemistry, 12, pp. 4361-4367
  • Russev, G., Tsanev, R., Determination of chain length of rat liver ribosomal RNA fractions (1975) C. R. Acad. Bulg. Sci., 28, pp. 807-810
  • Brawerman, G., Eukaryotic messenger RNA (1974) Annu. Rev. Biochem., 43, pp. 621-642
  • Perry, R.P., La Torre, J., Kelley, D.E., Greenberg, J.R., On the lability of poly (A) sequences during extraction of mRNA from polyribosomes (1972) Biophys. Biochim. Acta, 262, pp. 220-226
  • Spirin, A.S., Informosomes (1969) Eur. J. Biochem., 10, pp. 20-35
  • Nemer, M., Dubroff, L.M., Graham, M., Properties of sera urchin embryo messenger RNA containing and lacking poly (A) (1975) Cell, 6, pp. 171-178
  • Lim, L., Berthold, W., White, J., Hall, C., and Davison, A. N. 1975. Messenger RNA metabolism in the rat brain. Abstracts of Fifth International Meeting of the International Society for Neurochemistry, p. 279

Citas:

---------- APA ----------
Azcurra, J.M. & Carrasco, A.E. (1978) . Identification of polydisperse RNA in the cytoplasm of neurons isolated from the immature brain cortex. Neurochemical Research, 3(5), 521-534.
http://dx.doi.org/10.1007/BF00963756
---------- CHICAGO ----------
Azcurra, J.M., Carrasco, A.E. "Identification of polydisperse RNA in the cytoplasm of neurons isolated from the immature brain cortex" . Neurochemical Research 3, no. 5 (1978) : 521-534.
http://dx.doi.org/10.1007/BF00963756
---------- MLA ----------
Azcurra, J.M., Carrasco, A.E. "Identification of polydisperse RNA in the cytoplasm of neurons isolated from the immature brain cortex" . Neurochemical Research, vol. 3, no. 5, 1978, pp. 521-534.
http://dx.doi.org/10.1007/BF00963756
---------- VANCOUVER ----------
Azcurra, J.M., Carrasco, A.E. Identification of polydisperse RNA in the cytoplasm of neurons isolated from the immature brain cortex. Neurochem Res. 1978;3(5):521-534.
http://dx.doi.org/10.1007/BF00963756