Artículo

La versión final de este artículo es de uso interno de la institución.
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Exposure of the CNS to hypoxia is associated with cell death. Our aim was to establish a temporal correlation between cellular and molecular alterations induced by an acute hypoxia evaluated at different post-hypoxia (p-h) times and at two stages of chick optic lobe development: embryonic days (ED) 12 and 18. TUNEL assays at ED12 disclosed a significant increase (300%) in pyknotic cells at 6 h p-h, while at ED18 no morphological changes were observed in hypoxic versus controls. At ED12 there was a significant increase (48%) in Bcl-2 levels at the end of the hypoxic treatment, followed by a significant increase of active caspase-9 (49%) and active caspase-3 (58%) at 30 and 60 min p-h, respectively, while at ED18 no significant changes were observed. These findings indicate that prenatal hypoxia produces an equilibrated imbalance in both pro- and anti-apoptotic proteins that culminates in a process of cell death, present at earlier stages of development. © Springer Science+Business Media, Inc. 2006.

Registro:

Documento: Artículo
Título:Hypoxia-induced cell death and activation of pro- and anti-apoptotic proteins in developing chick optic lobe
Autor:Vacotto, M.; Paz, D.; De Plazas, S.F.
Filiación:Facultad de Medicina, Instituto de Biología Celular Y Neurociencias, Universidad de Buenos Aires, Paraguay 2155, 1121 Buenos Aires, Argentina
Facultad de Ciencias Exactas Y Naturales, Departamento de Biodiversidad Y Biología Experimental, Universidad de Buenos Aires, Paraguay 2155, 1121 Buenos Aires, Argentina
Palabras clave:Apoptosis; Caspase-3; Chick optic lobe; CNS development; Hypoxia; Programmed cell death; caspase 3; caspase 9; protein bcl 2; animal cell; animal tissue; article; brain development; brain hypoxia; chick embryo; controlled study; embryo; enzyme activity; nerve cell necrosis; nick end labeling; nonhuman; optic lobe; prenatal exposure; priority journal; Animals; Anoxia; Apoptosis; Apoptosis Regulatory Proteins; Caspase 3; Caspase 9; Chick Embryo; In Situ Nick-End Labeling; Optic Lobe; Optic Lobe, Nonmammalian; Proto-Oncogene Proteins c-bcl-2
Año:2006
Volumen:31
Número:8
Página de inicio:1003
Página de fin:1009
DOI: http://dx.doi.org/10.1007/s11064-006-9106-y
Título revista:Neurochemical Research
Título revista abreviado:Neurochem. Res.
ISSN:03643190
CODEN:NERED
CAS:caspase 3, 169592-56-7; caspase 9, 180189-96-2; protein bcl 2, 219306-68-0; Apoptosis Regulatory Proteins; Caspase 3, 3.4.22.-; Caspase 9, 3.4.22.-; Proto-Oncogene Proteins c-bcl-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03643190_v31_n8_p1003_Vacotto

Referencias:

  • Oppenheim, R.W., Cell death during development of the nervous system (1991) Annu Rev Neurosci, 14, pp. 453-501
  • Raff, M.C., Barres, B.A., Burne, J.F., Coles, H.S., Ishizaki, Y., Jaconson, M.D., Programmed cell death and the control of cell survival: Lesson from the nervous system (1993) Science, 262, pp. 695-700
  • Narayanan, V., Apoptosis in development and disease of the nervous system: 1. Naturally occurring cell death in the developing nervous system (1997) Pediatr Neurol, 16, pp. 9-13
  • Kuan, C.Y., Roth, K.A., Flavell, R.A., Rakic, P., Mechanisms of programmed cell death in the developing brain (2000) Trends Neuorosci, 23, pp. 291-297
  • Korsmeyer, S.J., BCL-2 gene family and the regulation of programmed cell death (1999) Cancer Res, 59, pp. S1693-S1700
  • Abedin, N., Ashraf, Q., Prakash Mishra, O., Delivoria-Papadopoulos, M., Effect of hypoxia on the expression of proand anti-apoptotic proteins in neuronal nuclei of the guinea pig fetus during gestation (2005) Brain Res Dev Brain Res, 156, pp. 32-37
  • Yang, J., Liu, X., Bhalla, K., Kim, C.N., Ibrado, A.M., Cai, J., Peng, T.I., Wang, X., Prevention of apoptosis by Bcl-2: Release of cytochrome c from mitochondria blocked (1997) Science, 275, pp. 1129-1132
  • Thornberry, N.A., Lazebnik, Y., Caspases: Enemies within (1998) Science, 281, pp. 1312-1316
  • Li, P., Nijhawan, D., Budihardjo, I., Srinivasula, S.M., Ahamad, M., Alnemri, E.S., Wang, X., Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade (1997) Cell, 91, pp. 479-489
  • Kuida, K., Haydar, T.F., Kuan, C.Y., Gu, Y., Taya, C., Karasuyama, H., Su, M.S., Flavell, R.A., Reduced apoptosis and cytochrome c-mediated caspase activation in mice lacking caspase 9 (1998) Cell, 94, pp. 325-337
  • Bossenmeyer-Pourie, C., Daval, J.L., Prevention from hypoxia-induced apoptosis by pre-conditioning: A mechanistic approach in cultured neurons from fetal rat forebrain (1998) Brain Res Mol Brain Res, 58, pp. 237-239
  • Cao, G., Minami, M., Pei, W., Yan, C., Chen, D., O'Horo, C., Graham, S.H., Chen, J., Intracellular Bax translocation after transient cerebral ischemia: Implications for a role of the mitochondrial apoptotic signaling pathway in ischemic neuronal death (2001) J Cereb Blood Flow Metab, 21, pp. 321-333
  • Khurana, P., Ashraf, Q.M., Mishra, O.P., Delivoria-Papadoupoulos, M., Effect of hypoxia on caspase-3, -8, and -9 activity and expression in the cerebral cortex of newborn piglets (2002) Neurochem Res, 27, pp. 931-938
  • Zhu, C., Wang, X., Hagberg, H., Blomgren, K., Correlation between caspase-3 activation and three different markers of DNA damage in neonatal cerebral hypoxia-ischemia (2000) J Neurochem, 75, pp. 819-829
  • Hu, B.R., Liu, C.L., Ouyang, Y., Blomgren, K., Siesjö, B., Involvement of caspase-3 in cell death after hypoxemia-ischemia declines during brain maturation (2000) J Cereb Blood Flow Metab, 20, pp. 1294-1300
  • Kelly, F.J., Free radical disorders of preterm infants (1993) Br Med Bull, 49, pp. 668-678
  • Vacotto, M., Rodrígez Gil, D.J., Mitridate De Novara, A., Fiszer De Plazas, S., Differential and irreversible CNS ontogenic reduction in maximal MK-801 binding site number in the NMDA receptor after acute hypoxic hypoxia (2003) Brain Res, 976, pp. 202-208
  • Gavrieli, Y., Sherman, Y., Ben-Sasson, S.A., Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation (1992) J Cell Biol, 119, pp. 493-501
  • Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, P.J., Protein measurement with Folin phenol reagent (1951) J Biol Chem, 193, pp. 265-275
  • Rego, A.C., Santos, M.S., Oliveira, C.R., Oxidative stress, hypoxia, and ischemia-like conditions increase the release of endogenous amino acids by distinct mechanisms in cultured retinal cells (1996) J Neurochem, 66, pp. 2506-2516
  • Saransaari, P., Oja, S.S., Release of endogenous glutamate, aspartate, GABA, and taurine from hippocampal slices from adult and developing mice under cell-damaging conditions (1998) Neurochem Res, 23, pp. 563-570
  • Dirnagal, U., Iadecola, C., Moskowitz, M.A., Pathobiology of ischaemic strke: An integrated view (1999) Trends Neurosci, 22, pp. 391-397
  • Walton, M., Sirimanne, E., Reutelingsperger, C., Williams, C., Gluckman, P., Dragunow, M., Annexin V labels apoptotic neurons following hypoxia-ischemia (1997) NeuroReport, 8, pp. 3871-3875
  • Fukuda, T., Wang, H., Nakanishi, H., Yamamoto, K., Kosaka, T., Novel non-apoptotic morphological changes in neurons of the mouse hippocampus following transient hypoxic-ischemia (1999) Neurosci Res, 33, pp. 49-55
  • Johnston, M.V., Selective vulnerability in the neonatal brain (1998) Ann Neurol, 44, pp. 155-156
  • Johnston, M.V., Nakajima, W., Hagberg, H., Mechanisms of hypoxic neurodegeneration in the developing brain (2002) Neuroscientist, 8, pp. 212-220
  • Vannucci, S.J., Harberg, H., Hypoxia-ischemia in the immature brain (2004) J Exp Biol, 207, pp. 3149-3154
  • Baumann, R., Haller, E.A., Schoning, U., Weber, M., Hypoxic incubation leads to concerted changes of carbonic anhydrase activity and 2.3 DPG concentration of chick embryo red cells (1986) Dev Biol, 116, pp. 548-551
  • Million, D., Zillner, P., Baumann, R., Oxygen pressure-dependent control of carbonic anhydrase synthesis in chick embryonic erythrocytes (1991) Am J Physiol, 261, pp. 1188-1196
  • Graham, S.H., Chen, J., Programmed cell death in cerebral ischemia (2001) J Cereb Blood Flow Metab, 21, pp. 99-109
  • Chen, Y., Ginis, I., Hallenbeck, J.M., The protective effect of ceramide in immature rat brain hypoxia-ischemia involves up-regulation of bcl-2 and reduction of TUNEL-positive cells (2001) J Cereb Blood Flow Metab, 21, pp. 34-40
  • Kaufmann, J.A., Perez, M., Zhang, W., Bickford, P.C., Holmes, D.B., Taglialatela, G., Free radical-dependent nuclear localization of Bcl-2 in the central nervous system of aged rats is not associated with Bcl-2-mediated protection from apoptosis (2003) J Neurochem, 81, pp. 981-994
  • Kelly, S., Zhao, H., Sun, G.H., Cheng, D., Qiao, Y., Luo, J., Martin, K., Yenari, M.A., Glycogen synthase kinase 3β inhibitor Chir025 reduces neuronal death resulting from oxygen-glucose deprivation, glutamate excitotoxicity, and cerebral ischemia (2004) Exp Neurol, 188, pp. 378-386
  • Wu, C., Fujihara, H., Yao, J., Qi, S., Li, H., Shimoji, K., Baba, H., Different expression patterns of Bcl-2, Bcl-xl, and Bax proteins after sublethal forebrain ischemia in C57Black/Crj6 mouse striatum (2003) Stroke, 34, pp. 1803-1808

Citas:

---------- APA ----------
Vacotto, M., Paz, D. & De Plazas, S.F. (2006) . Hypoxia-induced cell death and activation of pro- and anti-apoptotic proteins in developing chick optic lobe. Neurochemical Research, 31(8), 1003-1009.
http://dx.doi.org/10.1007/s11064-006-9106-y
---------- CHICAGO ----------
Vacotto, M., Paz, D., De Plazas, S.F. "Hypoxia-induced cell death and activation of pro- and anti-apoptotic proteins in developing chick optic lobe" . Neurochemical Research 31, no. 8 (2006) : 1003-1009.
http://dx.doi.org/10.1007/s11064-006-9106-y
---------- MLA ----------
Vacotto, M., Paz, D., De Plazas, S.F. "Hypoxia-induced cell death and activation of pro- and anti-apoptotic proteins in developing chick optic lobe" . Neurochemical Research, vol. 31, no. 8, 2006, pp. 1003-1009.
http://dx.doi.org/10.1007/s11064-006-9106-y
---------- VANCOUVER ----------
Vacotto, M., Paz, D., De Plazas, S.F. Hypoxia-induced cell death and activation of pro- and anti-apoptotic proteins in developing chick optic lobe. Neurochem. Res. 2006;31(8):1003-1009.
http://dx.doi.org/10.1007/s11064-006-9106-y