Abstract:
Exposure of the CNS to hypoxia is associated with cell death. Our aim was to establish a temporal correlation between cellular and molecular alterations induced by an acute hypoxia evaluated at different post-hypoxia (p-h) times and at two stages of chick optic lobe development: embryonic days (ED) 12 and 18. TUNEL assays at ED12 disclosed a significant increase (300%) in pyknotic cells at 6 h p-h, while at ED18 no morphological changes were observed in hypoxic versus controls. At ED12 there was a significant increase (48%) in Bcl-2 levels at the end of the hypoxic treatment, followed by a significant increase of active caspase-9 (49%) and active caspase-3 (58%) at 30 and 60 min p-h, respectively, while at ED18 no significant changes were observed. These findings indicate that prenatal hypoxia produces an equilibrated imbalance in both pro- and anti-apoptotic proteins that culminates in a process of cell death, present at earlier stages of development. © Springer Science+Business Media, Inc. 2006.
Registro:
Documento: |
Artículo
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Título: | Hypoxia-induced cell death and activation of pro- and anti-apoptotic proteins in developing chick optic lobe |
Autor: | Vacotto, M.; Paz, D.; De Plazas, S.F. |
Filiación: | Facultad de Medicina, Instituto de Biología Celular Y Neurociencias, Universidad de Buenos Aires, Paraguay 2155, 1121 Buenos Aires, Argentina Facultad de Ciencias Exactas Y Naturales, Departamento de Biodiversidad Y Biología Experimental, Universidad de Buenos Aires, Paraguay 2155, 1121 Buenos Aires, Argentina
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Palabras clave: | Apoptosis; Caspase-3; Chick optic lobe; CNS development; Hypoxia; Programmed cell death; caspase 3; caspase 9; protein bcl 2; animal cell; animal tissue; article; brain development; brain hypoxia; chick embryo; controlled study; embryo; enzyme activity; nerve cell necrosis; nick end labeling; nonhuman; optic lobe; prenatal exposure; priority journal; Animals; Anoxia; Apoptosis; Apoptosis Regulatory Proteins; Caspase 3; Caspase 9; Chick Embryo; In Situ Nick-End Labeling; Optic Lobe; Optic Lobe, Nonmammalian; Proto-Oncogene Proteins c-bcl-2 |
Año: | 2006
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Volumen: | 31
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Número: | 8
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Página de inicio: | 1003
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Página de fin: | 1009
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DOI: |
http://dx.doi.org/10.1007/s11064-006-9106-y |
Título revista: | Neurochemical Research
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Título revista abreviado: | Neurochem. Res.
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ISSN: | 03643190
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CODEN: | NERED
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CAS: | caspase 3, 169592-56-7; caspase 9, 180189-96-2; protein bcl 2, 219306-68-0; Apoptosis Regulatory Proteins; Caspase 3, 3.4.22.-; Caspase 9, 3.4.22.-; Proto-Oncogene Proteins c-bcl-2
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03643190_v31_n8_p1003_Vacotto |
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Citas:
---------- APA ----------
Vacotto, M., Paz, D. & De Plazas, S.F.
(2006)
. Hypoxia-induced cell death and activation of pro- and anti-apoptotic proteins in developing chick optic lobe. Neurochemical Research, 31(8), 1003-1009.
http://dx.doi.org/10.1007/s11064-006-9106-y---------- CHICAGO ----------
Vacotto, M., Paz, D., De Plazas, S.F.
"Hypoxia-induced cell death and activation of pro- and anti-apoptotic proteins in developing chick optic lobe"
. Neurochemical Research 31, no. 8
(2006) : 1003-1009.
http://dx.doi.org/10.1007/s11064-006-9106-y---------- MLA ----------
Vacotto, M., Paz, D., De Plazas, S.F.
"Hypoxia-induced cell death and activation of pro- and anti-apoptotic proteins in developing chick optic lobe"
. Neurochemical Research, vol. 31, no. 8, 2006, pp. 1003-1009.
http://dx.doi.org/10.1007/s11064-006-9106-y---------- VANCOUVER ----------
Vacotto, M., Paz, D., De Plazas, S.F. Hypoxia-induced cell death and activation of pro- and anti-apoptotic proteins in developing chick optic lobe. Neurochem. Res. 2006;31(8):1003-1009.
http://dx.doi.org/10.1007/s11064-006-9106-y