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Abstract:

Although the participation of the ubiquitin-dependent pathway and of the proteasome in apoptosis has been proposed, its role in this process is not yet clearly defined. In previous studies, we have shown that in the central nervous system of the rat, programmed cell death and the ubiquitin-dependent proteolytic pathway are closely related to each other and that different types of neurons and of glial cells, shown different types of correlation between the two phenomena. In this work, we have used lactacystin, a highly specific inhibitor of the proteasome, to explore in Schwann cell cultures the relationship between the activity of the Ub-dependent pathway and apoptosis. Apoptosis was explored analyzing changes in nuclear morphology, using the Annexin V assay and by flow cytometry. Activity of caspase-3 was also measured. Changes in the levels of ubiquitin-protein conjugates and of the ubiquitin activating enzymes, E1, as well as expression of proteins that instruct the cells to apoptosis (p53, NFκB-IκB, Bc12), or that participate in the control and regulation of the cell cycle, were also examined. Our results indicate that the decrease in the activity of the proteasome induced by lactacystin in Schwann cells, induces apoptotic cell death through changes in the concentration of certain key proteins that are involved in the apoptosis-signaling pathways.

Registro:

Documento: Artículo
Título:Apoptosis in Schwann cell cultures is closely interrelated with the activity of the ubiquitin-proteasome proteolytic pathway
Autor:Pasquini, L.A.; Besio Moreno, M.; Lopez Salon, M.; Soto, E.F.
Filiación:Inst. de Quim./Fisicoquim. Biologica, Departamento de Quimica Biologica, Universidad de Buenos Aires, Junin 956, Buenos Aires C1113AAD, Argentina
IQUIFIB (UBA-CONICET), Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, Junin 956, Buenos Aires C1113AAC, Argentina
Palabras clave:Apoptosis; Lactocystin; Proteasome; Schwann cells; Ubiquitin; caspase 3; I kappa B; immunoglobulin enhancer binding protein; lactacystin; lipocortin 5; proteasome; protein bcl 2; protein p53; ubiquitin; ubiquitin protein ligase; animal cell; apoptosis; article; cell cycle; cell structure; central nervous system; controlled study; correlation analysis; enzyme activity; flow cytometry; glia cell; measurement; nerve cell culture; nonhuman; priority journal; protein degradation; protein expression; rat; Schwann cell; signal transduction; Animals; Apoptosis; Cells, Cultured; Cysteine Endopeptidases; Flow Cytometry; Hydrolysis; Immunohistochemistry; Multienzyme Complexes; Proteasome Endopeptidase Complex; Rats; Rats, Wistar; Schwann Cells; Ubiquitin; Animalia
Año:2002
Volumen:27
Número:11
Página de inicio:1401
Página de fin:1419
DOI: http://dx.doi.org/10.1023/A:1021631901827
Título revista:Neurochemical Research
Título revista abreviado:Neurochem. Res.
ISSN:03643190
CODEN:NERED
CAS:caspase 3, 169592-56-7; lactacystin, 133343-34-7, 133398-98-8, 154333-21-8; lipocortin 5, 111237-10-6; proteasome, 140879-24-9; protein bcl 2, 219306-68-0; ubiquitin protein ligase, 134549-57-8; ubiquitin, 60267-61-0; Cysteine Endopeptidases, EC 3.4.22.-; Multienzyme Complexes; Proteasome Endopeptidase Complex, EC 3.4.25.1; Ubiquitin
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03643190_v27_n11_p1401_Pasquini

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Citas:

---------- APA ----------
Pasquini, L.A., Besio Moreno, M., Lopez Salon, M. & Soto, E.F. (2002) . Apoptosis in Schwann cell cultures is closely interrelated with the activity of the ubiquitin-proteasome proteolytic pathway. Neurochemical Research, 27(11), 1401-1419.
http://dx.doi.org/10.1023/A:1021631901827
---------- CHICAGO ----------
Pasquini, L.A., Besio Moreno, M., Lopez Salon, M., Soto, E.F. "Apoptosis in Schwann cell cultures is closely interrelated with the activity of the ubiquitin-proteasome proteolytic pathway" . Neurochemical Research 27, no. 11 (2002) : 1401-1419.
http://dx.doi.org/10.1023/A:1021631901827
---------- MLA ----------
Pasquini, L.A., Besio Moreno, M., Lopez Salon, M., Soto, E.F. "Apoptosis in Schwann cell cultures is closely interrelated with the activity of the ubiquitin-proteasome proteolytic pathway" . Neurochemical Research, vol. 27, no. 11, 2002, pp. 1401-1419.
http://dx.doi.org/10.1023/A:1021631901827
---------- VANCOUVER ----------
Pasquini, L.A., Besio Moreno, M., Lopez Salon, M., Soto, E.F. Apoptosis in Schwann cell cultures is closely interrelated with the activity of the ubiquitin-proteasome proteolytic pathway. Neurochem. Res. 2002;27(11):1401-1419.
http://dx.doi.org/10.1023/A:1021631901827