Abstract:
Although the participation of the ubiquitin-dependent pathway and of the proteasome in apoptosis has been proposed, its role in this process is not yet clearly defined. In previous studies, we have shown that in the central nervous system of the rat, programmed cell death and the ubiquitin-dependent proteolytic pathway are closely related to each other and that different types of neurons and of glial cells, shown different types of correlation between the two phenomena. In this work, we have used lactacystin, a highly specific inhibitor of the proteasome, to explore in Schwann cell cultures the relationship between the activity of the Ub-dependent pathway and apoptosis. Apoptosis was explored analyzing changes in nuclear morphology, using the Annexin V assay and by flow cytometry. Activity of caspase-3 was also measured. Changes in the levels of ubiquitin-protein conjugates and of the ubiquitin activating enzymes, E1, as well as expression of proteins that instruct the cells to apoptosis (p53, NFκB-IκB, Bc12), or that participate in the control and regulation of the cell cycle, were also examined. Our results indicate that the decrease in the activity of the proteasome induced by lactacystin in Schwann cells, induces apoptotic cell death through changes in the concentration of certain key proteins that are involved in the apoptosis-signaling pathways.
Registro:
Documento: |
Artículo
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Título: | Apoptosis in Schwann cell cultures is closely interrelated with the activity of the ubiquitin-proteasome proteolytic pathway |
Autor: | Pasquini, L.A.; Besio Moreno, M.; Lopez Salon, M.; Soto, E.F. |
Filiación: | Inst. de Quim./Fisicoquim. Biologica, Departamento de Quimica Biologica, Universidad de Buenos Aires, Junin 956, Buenos Aires C1113AAD, Argentina IQUIFIB (UBA-CONICET), Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, Junin 956, Buenos Aires C1113AAC, Argentina
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Palabras clave: | Apoptosis; Lactocystin; Proteasome; Schwann cells; Ubiquitin; caspase 3; I kappa B; immunoglobulin enhancer binding protein; lactacystin; lipocortin 5; proteasome; protein bcl 2; protein p53; ubiquitin; ubiquitin protein ligase; animal cell; apoptosis; article; cell cycle; cell structure; central nervous system; controlled study; correlation analysis; enzyme activity; flow cytometry; glia cell; measurement; nerve cell culture; nonhuman; priority journal; protein degradation; protein expression; rat; Schwann cell; signal transduction; Animals; Apoptosis; Cells, Cultured; Cysteine Endopeptidases; Flow Cytometry; Hydrolysis; Immunohistochemistry; Multienzyme Complexes; Proteasome Endopeptidase Complex; Rats; Rats, Wistar; Schwann Cells; Ubiquitin; Animalia |
Año: | 2002
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Volumen: | 27
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Número: | 11
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Página de inicio: | 1401
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Página de fin: | 1419
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DOI: |
http://dx.doi.org/10.1023/A:1021631901827 |
Título revista: | Neurochemical Research
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Título revista abreviado: | Neurochem. Res.
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ISSN: | 03643190
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CODEN: | NERED
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CAS: | caspase 3, 169592-56-7; lactacystin, 133343-34-7, 133398-98-8, 154333-21-8; lipocortin 5, 111237-10-6; proteasome, 140879-24-9; protein bcl 2, 219306-68-0; ubiquitin protein ligase, 134549-57-8; ubiquitin, 60267-61-0; Cysteine Endopeptidases, EC 3.4.22.-; Multienzyme Complexes; Proteasome Endopeptidase Complex, EC 3.4.25.1; Ubiquitin
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03643190_v27_n11_p1401_Pasquini |
Referencias:
- Sastry, P.S., Rao, K.S., Apoptosis and the nervous system (2000) J. Neurochem., 74, pp. 1-20
- Lossi, L., Zagzag, D., Greco, M.A., Merighi, A., Apoptosis of un-dedifferentiated progenitors and granule cell precursors on the postnatal human cerebellar cortex correlates with expression of BCL-25 ICE and CPP32 proteins (1998) J. Comp. Neurol., 399, pp. 359-372
- Imajoh-Ohmi, S., Kawaguchi, T., Sugiyama, S., Tanaka, K., Omura, S., Kikuchi, H., Lactacystin, a specific inhibitor of the proteasome, induces apoptosis in human monoblast U937 cells (1995) Biochem. Biophys. Res. Commun., 217, pp. 1070-1077
- Tanimoto, Y., Onishi, Y., Hashimoto, S., Kizabi, H., Peptidyl aldehyde inhibitors of proteasome induce apoptosis rapidly in mouse lymphoma RVC cells (1997) J. Biochem., 121, pp. 542-549
- Gross-Mesilaty, S., Basal and human papilloma virus E6 oncoprotein-induced degradation of Myc proteins by the ubiquitin pathway (1998) Proc. Natl. Acad. Sci., 95, pp. 8058-8063
- Pagano, M., Tam, S.W., Theodoras, A.M., Beer-Romero, P., Del Sal, G., Chau, V., Yew, P.R., Rolfe, M., Role of the ubiquitin-proteasome pathway in regulating abundance of the cyclin-dependent kinase inhibitor p27 (1995) Science, 269, pp. 682-685
- Pagano, M., Cell cycle regulation by the ubiquitin pathway (1997) FASEB J., 11, pp. 1067-1075
- Palombella, V., Rando, O.J., Goldberg, A.L., Mamates, T., The ubiquitin-proteasome pathway is required for processing the NFκB1 precursor protein and the activation of NFκB1 (1994) Cell, 78, pp. 773-785
- Gregory, M.A., Hann, S.R., c-Myc protelysis by the Ubiquitin-proteasome pathway: Stabilization of c-Myc in Burkitt's Lymphoma Cells (2000) Mol. Cell Biol., 20, pp. 2423-2435
- Maki, C.G., Huibregtse, J.M., Howley, P.M., In vivo ubiquitination and proteasome-mediated degradation of p53 (1996) Cancer Res., 56, pp. 2649-2654
- Stancovski, I., Gonen, H., Schwartz, O.A., Ciechanover, A., Degradation of the proto oncogene product cFos by the ubiquitin proteolytic system in vivo and in vitro: Identification and characterization of the conjugating enzymes (1995) Mol. Cell Biol., 15, pp. 7106-7116
- Hershko, A., Ciechanover, A., The Ubiqutin system (1998) Ann. Rev. Biochem., 67, pp. 425-479
- Ciechanover, A., The Ubiquitin-proteasome proteolytic pathway (1994) Cell, 79, pp. 13-21
- Shyne-Athwal, S., Riccio, R.V., Chakraboty, G., Ingoglia, N.A., Protein modification by amino acid addition is increased in crushed sciatic but not optic nerves (1986) Science, 231, pp. 603-605
- Berciano, T.M., Fernandez, R., Pena, E., Calle, E., Villagra, T.N., Lafarga, M., Necrosis of SC during Tellurium-induced primary demyelination: DNA fragmentation, reorganization of splicing machinery, and formation of intranuclear rods of actin (1999) J. Neuropathol. Exp. Neurol., 58, pp. 1234-1243
- Calle, E., Berciano, M.T., Fernandez, R., Lafarga, M., Activation of the autophagy, c-Fos and ubiquitin expression, and nucleolar alterations in Schwann cells precede demyelination in tellurium-induced neuropathy (1998) Acta. Neuropathol., 97, pp. 143-155
- Nakao, J., Shinoda, J., Nakai, Y., Murase, S., Uyemura, K., Apoptosis regulates the number of Shwann cells at the premyelinating stage (1997) J. Neurochem., 68, pp. 1853-1862
- Fenteany, G., Standaert, R.F., Lane, W.S., Choi, S., Corey, E.J., Schreiber, S.L., Inhibition of proteasome activities and subunit specific-amino terminal threonine modification by lactocystin (1995) Science, 268, pp. 726-731
- Fenteany, G., Schreiber, S.L., Lactacystin, proteasome function and cell fate (1998) J. Biol. Chem., 273, pp. 8545-8548
- Pasquini, L.A., Besio Moreno, M.A.N., Adamo, A.M., Pasquini, J.M., Soto, E.F., Lactacystin, a specific inhibitor of the proteasome, induces apoptosis and activates caspase-3 in cultured cerebelar granule cells (2000) J. Neurosc. Res., 59, pp. 601-611
- Pasquini, L.A., Marta, C., Adamo, A.M., Pasquini, J.M., Soto, E.F., Relationship between the ubiquitin-dependent pathway and apoptosis in different cells the central nervous system; effect of thyroid hormones (2000) Neurochem. Res. 25, 5, pp. 627-635
- López Salon, M., Morelli, L., Castaño, E.M., Soto, E.F., Pasquini, J.M., Defective ubiquitination of cerebral proteins in Alzheimer's Disease (2000) J. Neurosc. Res., 63, pp. 302-310
- Kreider, B.Q., Messing, A., Doan, H., Kim, S.U., Lisak, R.P., Pleasure, D.E., Enrichment of Schwann cells cultures from neonatal rat sciatic nerve by differential adhesion (1981) Brain Research, 207, pp. 433-444
- Distelhorst, C.W., Mc Cormick, T.S., Bcl-2 acts subsequent to and independent of Ca2+ fluxes to inhibit apoptosis in thapsigargin and glucocorticoid-treated mouse lymphoma cells (1996) Cell Calcium, 19, pp. 473-483
- Lowry, O.R., Rosebrough, N.R., Farr, A., Land Randall, R.J., Protein measurement with Folin phenol reagent (1951) J. Biol. Chem., 193, pp. 265-275
- Laemmli, U.K., Clevage of structural proteins the assembly of the head of bacteriophage T4 (1970) Nature, 227, pp. 680-685
- Williams, G.T., Smith, C.A., Molecular regulation of apoptosis. Genetic control on cell death (1993) Cell, 74, pp. 777-779
- Beyette, J., Mason, G.G.F., Murray, R.Z., Cohen, G.M., Rivett, A.J., Proteasome activities decrease during dexamethasone-induced apoptosis of thymocytes (1998) Biochem. J., 332, pp. 315-320
- Chandra, J., Niemer, I., Gilbreath, J., Kliche, K.O., Andreeff, M., Freireich, E.J., Keating, M., Mc Conkey, D.J., Proteasome inhibitors induce apoptosis in glucocorticoid-resistant chronic lymphocytic leukemic lymphocytes (1998) Blood, 92, pp. 4220-4229
- Dickson, D.W., Apoptosis in the brain: Physiology and pathology (1995) J. Pathology, 146, pp. 1040-1044
- Drexler, H.C., Activation of the cell death program by inhibition of proteasome function (1997) Proc. Natl. Acad. Sci., 94, pp. 855-860
- Shinohara, K., Tomioka, M., Nakano, H., Tone, S., Ito, H., Kawashima, S., Apoptosis induction resulting from proteasome inhibition (1996) Biochem. J., 317, pp. 385-388
- Stefanelli, C., Bonavita, F., Stanic, I., Pignatti, C., Farruggia, G., Masotti, L., Guarnieri, C., Caldarera, C.M., Inhibition of etoposide-induced apoptosis with peptide aldehyde inhibitors of proteasome (1998) Biochem. J., 332, pp. 661-665
- Soldatenkov, V.A., Dritschilo, A., Apoptosis of Ewing's sarcoma cells is accompanied by accumulation of ubiquitinated proteins (1997) Cancer Res., 57, pp. 3881-3885
- Araki, T., Nagarajan, R., Milbrandt, J., Identification of genes induced in peripheral nerve after injury. Expression profiling and novel gene discovery (2001) J. Biol. Chem., 276, pp. 34131-34141
- Grenfell, S.J., Trausch-Azar, J.S., Handley-Gearhart, P.M., Ciechanover, A., Schwartz, A.L., Nuclear localization of the ubiquitin activating enzyme, E1, is cell-cycle dependent (1994) Biochem. J., 300, pp. 701-708
- Elledge, S., Harper, W., Cdk inhibitors: On the threshold of checkspoints and development (1994) Curr. Opin. Cell Biol., 6, pp. 847-852
- King, R.W., Deshaies, R.J., Peters, J.M., Kirschner, M.W., How proteolysis drives the cell cycle (1996) Science, 274, pp. 1652-1658
- Diehl, J.A., Zindy, F., Sherr, C.J., Inhibition of cyclin D1 phosphorylation on threonine 286 prevents its rapid degradation via the ubiquitin-proteasome pathway (1997) Genes Dev., 11, pp. 957-972
- Adamo, A.M., Pasquini, L.A., Besio Moreno, M., Oteiza, P.I., Soto, E.F., Pasquini, J.M., Effect of oxidant systems on the ubiquitylation of proteins in the central nervous system (1999) J. Neurosci. Res., 55, pp. 523-531
- Nigg, E.A., Cyclin-dependent protein kinase: Key regulators of the eukaryotic cell cycle (1995) BioEssays, 17, pp. 471-480
- Sudakin, V., Ganoth, D., Dahan, A., Heller, H., Hershko, J., Luca, F.C., Ruderman, J.V., Hershko, A., The cyclosome, a large complex containing cyclin-selective ubiquitin ligase activity targets cyclins for destruction at the end of mitosis (1995) Mol. Biol. Cell, 6, pp. 185-197
- ElShamy, W.M., Fridvall, L.K., Ernfors, P., Growth arrest failure G1 restriction point override and S phase death of sensory precursor cells in the absence of neurotrophin-3 (1998) Neuron, 21, pp. 1003-1015
- Casaccia-Bonnefil, P., Cell death in the oligodendrocytes lineage: A molecular perspective of life/death decisions in development and disease (2000) Glia, 29, pp. 124-135
- Baeuerle, P.A., Baltimore, D., NF-kappa B: Ten years after (1996) Cell, 87, pp. 13-20
- Moissac, D., Mustapha, S., Greenberg, A., Kirshenbaum, L., Bcl-2 activates the transcription factor NFκB through the degradation of the cytoplasmic inhibitor IκBα (1998) J. Biol. Chem., 273, pp. 23946-23951
- Pasquini, L.A., López Salon, M., Besio Moreno, M., Soto, E.F., Pasquini, J.M., Participation of the proteolytic pathway mediated by ubiquitin in the activation of NFκB by ceramide in primary cultures of astrocytes (2000) J. Neurochem., 74, pp. S8C
Citas:
---------- APA ----------
Pasquini, L.A., Besio Moreno, M., Lopez Salon, M. & Soto, E.F.
(2002)
. Apoptosis in Schwann cell cultures is closely interrelated with the activity of the ubiquitin-proteasome proteolytic pathway. Neurochemical Research, 27(11), 1401-1419.
http://dx.doi.org/10.1023/A:1021631901827---------- CHICAGO ----------
Pasquini, L.A., Besio Moreno, M., Lopez Salon, M., Soto, E.F.
"Apoptosis in Schwann cell cultures is closely interrelated with the activity of the ubiquitin-proteasome proteolytic pathway"
. Neurochemical Research 27, no. 11
(2002) : 1401-1419.
http://dx.doi.org/10.1023/A:1021631901827---------- MLA ----------
Pasquini, L.A., Besio Moreno, M., Lopez Salon, M., Soto, E.F.
"Apoptosis in Schwann cell cultures is closely interrelated with the activity of the ubiquitin-proteasome proteolytic pathway"
. Neurochemical Research, vol. 27, no. 11, 2002, pp. 1401-1419.
http://dx.doi.org/10.1023/A:1021631901827---------- VANCOUVER ----------
Pasquini, L.A., Besio Moreno, M., Lopez Salon, M., Soto, E.F. Apoptosis in Schwann cell cultures is closely interrelated with the activity of the ubiquitin-proteasome proteolytic pathway. Neurochem. Res. 2002;27(11):1401-1419.
http://dx.doi.org/10.1023/A:1021631901827