Artículo

González Deniselle, M.C.; Lavista-Llanos, S.; Ferrini, M.G.; Lima, A.E.; Roldán, A.G.; De Nicola, A.F. "In vitro differences between astrocytes of control and Wobbler mice spinal cord" (1999) Neurochemical Research. 24(12):1535-1541
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Abstract:

The Wobbler mouse, a model of amyotrophic lateral sclerosis (ALS), presents motorneuron degeneration and pronounced astrogliosis in the spinal cord. We have studied factors controlling astrocyte proliferation in cultures derived from Wobbler and control mice spinal cord. Basal rate of [3H]thymidine incorporation was 15 times lower in Wobbler astrocytes. While in control cultured cells interleukin-1α (IL-1) and corticosterone (CORT) significantly increased proliferation, both agents were inactive in Wobbler astrocytes. The lack of response to CORT was not due to the absence of glucocorticoid receptors, because similar receptor amounts were found in Wobbler and control astrocytes. In contrast to IL-1 and CORT, transforming growth factor-β1 (TGF-β1) substantially increased proliferation of Wobbler astrocytes but not of control cells. Differences in response to TGF- β1 were also obtained by measuring glial fibrillary acidic protein (GFAP) immunoreaction intensity, which was substantially higher in Wobbler astrocytes. Thus, abnormal responses to different mitogens characterized Wobbler astrocytes in culture. We suggest that TGF-β1 may play a role in the reactive gliosis and GFAP hyperexpression found in the degenerating spinal cord of this model of ALS.

Registro:

Documento: Artículo
Título:In vitro differences between astrocytes of control and Wobbler mice spinal cord
Autor:González Deniselle, M.C.; Lavista-Llanos, S.; Ferrini, M.G.; Lima, A.E.; Roldán, A.G.; De Nicola, A.F.
Filiación:Inst. de Biologia y Med. Exp., Buenos Aires, Argentina
Inst. Univ. de Ciencias de la Salud, Fundación Barceló, Buenos Aires, Argentina
Lab. of Neuroendocrine Biochemistry, Inst. de Biol. Y Med. Experimental, Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:Astrocytes; Culture; Glial fibrillary acidic protein; Glucocorticoids; Interleukin 1; TGF-β1; Wobbler mouse; corticosterone; glial fibrillary acidic protein; interleukin 1alpha; transforming growth factor beta1; amyotrophic lateral sclerosis; animal cell; article; astrocyte; astrocytosis; cell proliferation; controlled study; female; male; motoneuron; mouse; nerve cell degeneration; nonhuman; priority journal; protein expression; spinal cord atrophy; Animals; Astrocytes; Cell Division; Cells, Cultured; Corticosterone; Female; Glial Fibrillary Acidic Protein; Gliosis; Interleukin-1; Kinetics; Male; Mice; Mice, Inbred Strains; Mice, Neurologic Mutants; Motor Neuron Disease; Neuroprotective Agents; Pregnatrienes; Receptors, Glucocorticoid; Reference Values; Spinal Cord; Thymidine; Transforming Growth Factor beta; Animalia
Año:1999
Volumen:24
Número:12
Página de inicio:1535
Página de fin:1541
Título revista:Neurochemical Research
Título revista abreviado:Neurochem. Res.
ISSN:03643190
CODEN:NERED
CAS:Corticosterone, 50-22-6; Glial Fibrillary Acidic Protein; Interleukin-1; Neuroprotective Agents; Pregnatrienes; Receptors, Glucocorticoid; Thymidine, 50-89-5; Transforming Growth Factor beta; U 74389F, 111668-89-4
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03643190_v24_n12_p1535_GonzalezDeniselle

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Citas:

---------- APA ----------
González Deniselle, M.C., Lavista-Llanos, S., Ferrini, M.G., Lima, A.E., Roldán, A.G. & De Nicola, A.F. (1999) . In vitro differences between astrocytes of control and Wobbler mice spinal cord. Neurochemical Research, 24(12), 1535-1541.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03643190_v24_n12_p1535_GonzalezDeniselle [ ]
---------- CHICAGO ----------
González Deniselle, M.C., Lavista-Llanos, S., Ferrini, M.G., Lima, A.E., Roldán, A.G., De Nicola, A.F. "In vitro differences between astrocytes of control and Wobbler mice spinal cord" . Neurochemical Research 24, no. 12 (1999) : 1535-1541.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03643190_v24_n12_p1535_GonzalezDeniselle [ ]
---------- MLA ----------
González Deniselle, M.C., Lavista-Llanos, S., Ferrini, M.G., Lima, A.E., Roldán, A.G., De Nicola, A.F. "In vitro differences between astrocytes of control and Wobbler mice spinal cord" . Neurochemical Research, vol. 24, no. 12, 1999, pp. 1535-1541.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03643190_v24_n12_p1535_GonzalezDeniselle [ ]
---------- VANCOUVER ----------
González Deniselle, M.C., Lavista-Llanos, S., Ferrini, M.G., Lima, A.E., Roldán, A.G., De Nicola, A.F. In vitro differences between astrocytes of control and Wobbler mice spinal cord. Neurochem. Res. 1999;24(12):1535-1541.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03643190_v24_n12_p1535_GonzalezDeniselle [ ]