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Abstract:

Several lines of evidence indicate that protein kinase C (PKC) is involved in long-term potentiation (LTP) and in certain forms of learning. Recently, we found a learning-specific, time-dependent increase in [3H]phorbol dibutyrate binding to membrane-associated PKC in the hippocampus of rats subjected to an inhibitory avoidance task. Here we confirm and extend this observation, describing that a one trial inhibitory avoidance learning was associated with rapid and specific increases in B-50/GAP-43 phosphorylation in vitro and in PKC activity in hippocampal synaptosomal membranes. The increased phosphorylation of B-50/GAP-43 was seen at 30 rain (+35% relative to naive or shocked control groups), but not at 10 or 60 rain after training. This learning-associated increase in the phosphorylation of B-50/GAP-43 is mainly due to an increase in the activity of PKC. This is based on three different sets of data: 1) PKC activity increased by 24% in hippocampal synaptosomal membranes of rats sacrificed 30 min after training; 2) B-50/GAP-43 immunoblots revealed no changes in the amount of this protein among the different experimental groups; 3) phosphorylation assays, performed in the presence of bovine purified PKC or in the presence of the selective PKC inhibitor CGP 41231, exhibited no differences in B-50/GAP-43 phosphorylation between naive and trained animals. In conclusion, these results support the contention that hippocampal PKC participates in the early neural events of memory formation of an aversively-motivated learning task.

Registro:

Documento: Artículo
Título:B-50/GAP-43 phosphorylation and PKC activity are increased in rat hippocampal synaptosomal membranes after an inhibitory avoidance training
Autor:Cammarota, M.; Paratcha, G.; De Stein, M.L.; Bernabeu, R.; Izquierdo, I.; Medina, J.H.
Filiación:Instituto de Biología Celular y Neurociencias, Facultad de Medicina, UBA, Buenos Aires, Argentina
Laboratorio de Biología Celular, Depto. Biología, FCEyN, Buenos Aires, Argentina
Centro de Memoria, Instituto de Biociencias, UFRGS, Porto Alegre, Brazil
Instituto de Biología Celular y Neurociencias, Facultad de Medicina, UBA, Paraguay 2155, (1121) Buenos Aires, Argentina
Palabras clave:B-50/GAP-43; hippocampus; learning; memory; PKC; protein phosphorylation; 1 (5 isoquinolinesulfonyl) 2 methylpiperazine; neuromodulin; phorbol dibutyrate; polymyxin B; protein kinase C; protein kinase C inhibitor; staurosporine derivative; tritium; animal cell; animal experiment; article; brain synaptosome; controlled study; enzyme activity; hippocampus; learning; long term potentiation; male; memory; nonhuman; priority journal; protein phosphorylation; rat; synaptic membrane; task performance; training; Animalia; Bovinae; Tritium
Año:1997
Volumen:22
Número:4
Página de inicio:499
Página de fin:505
DOI: http://dx.doi.org/10.1023/A:1027324214060
Título revista:Neurochemical Research
Título revista abreviado:NEUROCHEM. RES.
ISSN:03643190
CODEN:NERED
CAS:1 (5 isoquinolinesulfonyl) 2 methylpiperazine, 84477-87-2; phorbol dibutyrate, 61557-88-8; polymyxin B, 1404-26-8, 1405-20-5; protein kinase C, 141436-78-4; protein kinase C theta; tritium, 10028-17-8
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03643190_v22_n4_p499_Cammarota

Referencias:

  • Bliss, T.P.V., Collingridge, B.L., A synaptic model of memory: Long-term potentiation in the hippocampus (1993) Nature, 361, pp. 31-39
  • Izquierdo, I., Medina, J.H., Correlation between the pharmacology of long-term potentiation and the pharmacology of memory (1995) Neurobiol. Learning & Memory, 63, pp. 19-24
  • Akers, R.F., Lovinger, D.M., Colley, P.A., Linden, D.J., Routtemberg, A., Translocation of PKC activity may mediate hippocampal long-term potentiation (1986) Science, 231, pp. 587-589
  • Malinow, R., Madison, D.V., Tsien, R.V., Persistent PKC activity underlying long-term potentiation (1988) Nature, 335, pp. 820-824
  • Abeliovich, A., Paylor, R., Chen, C., Kim, J.J., Wehner, J.M., Tonegawa, S., PKCγ-mutant mice exhibit mild deficits in spatial and contextual learning (1993) Cell, 75, pp. 1263-1271
  • Ramakers, G.M.J., De Graan, P.N.E., Urban, I.J.A., Kiaay, D., Tang, T., Pasinelli, P., Oestreicher, A.B., Gispen, W.H., Temporal differences in the phosphorylation state of pre- And post-synaptic PKC substrates B-50/GAP-43 and neurogranin during long-term potentiation (1995) J. Biol. Chem., 270, pp. 13892-13898
  • Akers, R., Routtemberg, A., PKC phosphorylates a 47 Kd protein (F1) directly related to synaptic plasticity (1985) Brain Res., 334, pp. 147-151
  • Nelson, R.B., Linden, D.J., Routtenberg, A., Phosphoproteins localized to presynaptic terminal linked to persistence of long-term potentiation (LTP): Quantitative analysis of two dimensional gels (1989) Brain Res., 497, pp. 30-42
  • Gianotti, C., Nunai, M.G., Gispen, W.H., Corradetti, R., Phosphorylation of the presynaptic protein B-50 (GAP-43) is increased during electrically induced long-term potentiation (1992) Neuron., 8, pp. 843-848
  • Gispen, W.H., Nielander, H.B., De Graan, P.N.E., Oestreicher, A.B., Schram, L.H., Schotman, P., Role of the growth-associated protein B-50/GAP-43 in neuronal plasticity (1991) Mol. Neurobiol., 5, pp. 61-85
  • Strittmatter, S.M., Vartanian, T., Fischman, M.C., GAP-43 as a plasticity protein in neuronal form and repair (1992) J. Neurobiol., 23, pp. 507-520
  • Burchuladze, R., Potter, J., Rose, S.P.R., Memory formation: The chick depends on membrane-bound PKC (1990) Brain Res., 535, pp. 131-138
  • Jerusalinsky, D., Quillfeldt, J.A., Walz, R., Da Silva, R.C., Medina, J.H., Izquierdo, I., Post-training intrahippocampal infusion of PKC inhibitors causes amnesia in the rat (1994) Behav. Neural Biol., 61, pp. 107-109
  • Jerusalinsky, D., Quillfeldt, J.A., Walz, R., Da Silva, R.C., Medina, J.H., Izquierdo, I., Infusion of a PKC inhibitor into the amygdala or entorhinal cortex causes retrograde amnesia in rats (1994) Commun. Biol., 11, pp. 179-185
  • Scharenberg, A.M., Olds, J.L., Schereurs, B.G., Craig, A.M., Alkon, D.L., PKC redistribution within CA3 stratum oriens during acquisition of nictitating membrane conditioning in the rabbit (1991) Proc. Natl. Acad. Sci. USA, 88, pp. 6637-6641
  • Sunayashi-Kusuzaki, K., Lester, D.S., Schreurs, B.G., Alkon, D.L., Associative learning potentiates PKC activation in synaptosomes of the rabbit hippocampus (1993) Proc. Natl. Acad. Sci. USA, 90, pp. 4286-4289
  • Bernabeu, R., Izquierdo, I., Cammarota, M., Jerusalinky, D., Medina, J.H., Learning-specific, time-dependent increase in [3H]phorbol dibutyrate binding to PKC in selected regions of rat brain (1995) Brain Res., 685, pp. 163-168
  • De Robertis, E., Pellegrino De Iraldi, A., Rodriguez De Lores De Arnaiz, G., Gomez, C.J., On the isolation of nerve endings and synaptic vesicles (1961) J. Biophys. Biochem. Cytol., 9, pp. 229-235

Citas:

---------- APA ----------
Cammarota, M., Paratcha, G., De Stein, M.L., Bernabeu, R., Izquierdo, I. & Medina, J.H. (1997) . B-50/GAP-43 phosphorylation and PKC activity are increased in rat hippocampal synaptosomal membranes after an inhibitory avoidance training. Neurochemical Research, 22(4), 499-505.
http://dx.doi.org/10.1023/A:1027324214060
---------- CHICAGO ----------
Cammarota, M., Paratcha, G., De Stein, M.L., Bernabeu, R., Izquierdo, I., Medina, J.H. "B-50/GAP-43 phosphorylation and PKC activity are increased in rat hippocampal synaptosomal membranes after an inhibitory avoidance training" . Neurochemical Research 22, no. 4 (1997) : 499-505.
http://dx.doi.org/10.1023/A:1027324214060
---------- MLA ----------
Cammarota, M., Paratcha, G., De Stein, M.L., Bernabeu, R., Izquierdo, I., Medina, J.H. "B-50/GAP-43 phosphorylation and PKC activity are increased in rat hippocampal synaptosomal membranes after an inhibitory avoidance training" . Neurochemical Research, vol. 22, no. 4, 1997, pp. 499-505.
http://dx.doi.org/10.1023/A:1027324214060
---------- VANCOUVER ----------
Cammarota, M., Paratcha, G., De Stein, M.L., Bernabeu, R., Izquierdo, I., Medina, J.H. B-50/GAP-43 phosphorylation and PKC activity are increased in rat hippocampal synaptosomal membranes after an inhibitory avoidance training. NEUROCHEM. RES. 1997;22(4):499-505.
http://dx.doi.org/10.1023/A:1027324214060