Artículo

Tresguerres, M.; Parks, S.K.; Sabatini, S.E.; Goss, G.G.; Luquet, C.M. "Regulation of ion transport by pH and [HCO3 -] in isolated gills of the crab Neohelice (Chasmagnathus) granulata" (2008) American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 294(3):R1033-R1043
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Abstract:

Posterior isolated gills of Neohelice (Chasmagnathus) granulatus were symmetrically perfused with hemolymph-like saline of varying [HCO 3 -] and pH. Elevating [HCO3 -] in the saline from 2.5 to 12.5 mmol/l (pH 7.75 in both cases) induced a significant increase in the transepithelial potential difference (Vte), a measure of ion transport. The elevation in [HCO3 -] also induced a switch from acid secretion (-43.7 ± 22.5 μequiv·kg -1·h-1) in controls to base secretion (84.7 ± 14.4 μequiv·kg-1·h-1). The HCO3 --induced Vte increase was inhibited by basolateral acetazolamide (200 μmol/l), amiloride (1 mmol/l), and ouabain (5 mmol/l) but not by bafilomycin (100 nmol/l). The Vte response to HCO3 - did not take place in Cl--free conditions; however, it was unaffected by apical SITS (2 mmol/l) or DIDS (1 mmol/l). A decrease in pH from 7.75 to 7.45 pH units in the perfusate also induced a significant increase in Vte, which was matched by a net increase in acid secretion of 67.8 ± 18.4 μequiv kg-1 h-1. This stimulation was sensitive to basolateral acetazolamide, bafilomycin, DIDS, and Na+-free conditions, but it still took place in Cl--free saline. Therefore, the cellular response to low pH is different from the HCO3 --stimulated response. We also report V-H+-ATPase- and Na+-K+-ATPase-like immunoreactivity in gill sections for the first time in this crab. Our results suggest that carbonic anhydrase (CA), basolateral Na+/H+ exchangers and Na+-K+-ATPase and apical anion exchangers participate in the HCO3 --stimulated response, while CA, apical V-H+-ATPase and basolateral HCO3 --dependent cotransporters mediate the response to low pH. Copyright © 2008 the American Physiological Society.

Registro:

Documento: Artículo
Título:Regulation of ion transport by pH and [HCO3 -] in isolated gills of the crab Neohelice (Chasmagnathus) granulata
Autor:Tresguerres, M.; Parks, S.K.; Sabatini, S.E.; Goss, G.G.; Luquet, C.M.
Filiación:Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada
Department of Pharmacology, Weill Medical College of Cornell University, New York, NY, United States
Consejo Nacional de Investigaciones Científicas Y Técnicas, Buenos Aires, Argentina
Departamento Biodiversidad Y Biología Experimental, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Asentamiento Universitario San Martín de Los Andes, Neuquén, Argentina
Dept. of Pharmacology, Weill Medical College of Cornell Univ., New York, NY 10021, United States
Palabras clave:Acetazolamide; Acid/base regulation; Amiloride; Bafilomycin; Carbonic anhydrase; Electrogenic Na+/H+ exchanger; HCO3 - secretion; V-H +-ATPase; acetazolamide; adenosine triphosphatase (potassium sodium); amiloride; bafilomycin; bicarbonate; carbonate dehydratase; ouabain; proton transporting adenosine triphosphatase; sodium chloride; sodium proton exchange protein; acid base balance; acid secretion; acidity; alkalinity; animal experiment; animal tissue; article; cell membrane potential; crab; epithelium cell; gill; immunoreactivity; ion transport; Neohelice granulata; nonhuman; perfusion; priority journal; 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; Animals; Bicarbonates; Biological Transport, Active; Brachyura; Diuretics; Enzyme Inhibitors; Ganglia, Invertebrate; Gills; Hydrogen-Ion Concentration; Immunohistochemistry; Indicators and Reagents; Ions; Macrolides; Membrane Potentials; Proton-Translocating ATPases; Solutions
Año:2008
Volumen:294
Número:3
Página de inicio:R1033
Página de fin:R1043
DOI: http://dx.doi.org/10.1152/ajpregu.00516.2007
Título revista:American Journal of Physiology - Regulatory Integrative and Comparative Physiology
Título revista abreviado:Am. J. Physiol. Regul. Integr. Comp. Physiol.
ISSN:03636119
CODEN:AJPRD
CAS:acetazolamide, 1424-27-7, 59-66-5; amiloride, 2016-88-8, 2609-46-3; bicarbonate, 144-55-8, 71-52-3; carbonate dehydratase, 9001-03-0; ouabain, 11018-89-6, 630-60-4; sodium chloride, 7647-14-5; sodium proton exchange protein, 204597-32-0; 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid, 53005-05-3; 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid, 27816-59-7; bafilomycin A, 116764-51-3; Bicarbonates; Diuretics; Enzyme Inhibitors; Indicators and Reagents; Ions; Macrolides; Proton-Translocating ATPases, EC 3.6.3.14; Solutions
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03636119_v294_n3_pR1033_Tresguerres

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Citas:

---------- APA ----------
Tresguerres, M., Parks, S.K., Sabatini, S.E., Goss, G.G. & Luquet, C.M. (2008) . Regulation of ion transport by pH and [HCO3 -] in isolated gills of the crab Neohelice (Chasmagnathus) granulata. American Journal of Physiology - Regulatory Integrative and Comparative Physiology, 294(3), R1033-R1043.
http://dx.doi.org/10.1152/ajpregu.00516.2007
---------- CHICAGO ----------
Tresguerres, M., Parks, S.K., Sabatini, S.E., Goss, G.G., Luquet, C.M. "Regulation of ion transport by pH and [HCO3 -] in isolated gills of the crab Neohelice (Chasmagnathus) granulata" . American Journal of Physiology - Regulatory Integrative and Comparative Physiology 294, no. 3 (2008) : R1033-R1043.
http://dx.doi.org/10.1152/ajpregu.00516.2007
---------- MLA ----------
Tresguerres, M., Parks, S.K., Sabatini, S.E., Goss, G.G., Luquet, C.M. "Regulation of ion transport by pH and [HCO3 -] in isolated gills of the crab Neohelice (Chasmagnathus) granulata" . American Journal of Physiology - Regulatory Integrative and Comparative Physiology, vol. 294, no. 3, 2008, pp. R1033-R1043.
http://dx.doi.org/10.1152/ajpregu.00516.2007
---------- VANCOUVER ----------
Tresguerres, M., Parks, S.K., Sabatini, S.E., Goss, G.G., Luquet, C.M. Regulation of ion transport by pH and [HCO3 -] in isolated gills of the crab Neohelice (Chasmagnathus) granulata. Am. J. Physiol. Regul. Integr. Comp. Physiol. 2008;294(3):R1033-R1043.
http://dx.doi.org/10.1152/ajpregu.00516.2007