Artículo

Mac Laughlin, M.; Damasco, M.C.; Igarreta, P.; Amorena, C. "In vitro and in vivo evaluation of proximal tubular acidification in aging rats" (2001) American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 280(6 49-6):R1627-R1631
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Abstract:

The normal aging process is accompanied by a progressive deterioration of renal function. We studied the kinetics of proximal tubular acidification of young (3 mo) and aging (22 mo) rats using in vivo and in vitro techniques. Blood acid-base parameters were similar in both groups. The maximum velocity of the Na+/H+ exchange (NHE) in brush-border membrane vesicles (BBMV) showed a 72% decrease in aging compared with young rats, whereas the Michaelis constant remained unchanged. The NHE3 isoform of the Na+/H+ exchanger was detected in BBMV by Western blot in both groups, and a decrease of 90% in the abundance was observed in aging rats. Micropuncture experiments with simultaneous luminal and peritubular perfusion with phosphate Ringer and continuous measurement of intratubular pH showed an acidification rate constant 34% smaller in aging compared with young rats. Proton flux was 48% lower in aging than in young rats. The present results suggest that proximal tubular acidification is impaired with aging.

Registro:

Documento: Artículo
Título:In vitro and in vivo evaluation of proximal tubular acidification in aging rats
Autor:Mac Laughlin, M.; Damasco, M.C.; Igarreta, P.; Amorena, C.
Filiación:Instituto de Investigaciones Cardiológicas, Facultad de Medicina, 1122 Buenos Aires, Argentina
Programa de Regulacion Hormonal y Metabólica, Consejo National de Investigaciones Centíficas y Técnicas, 1428 Universidad de Buenos Aires, Buenos Aires, Argentina
Escuela de Ciencia y Tecnología, Universidad National de Gral San Martín, 1650 San Martín, Argentina
Instituto de Investigaciones Cardiológicas, M. T. de Alvear 2270, 1122 Buenos Aires, Argentina
Palabras clave:Micropuncture; Na+/H+; Vesicles; acidification; aging; animal cell; animal experiment; animal tissue; article; blood pH; brush border; brush border vesicle; controlled study; disease course; kidney function; kidney proximal tubule; male; Michaelis constant; nonhuman; priority journal; proton transport; punch biopsy; rat; Acids; Aging; Animals; Bicarbonates; Blotting, Western; Hydrogen-Ion Concentration; Kidney Tubules, Proximal; Kinetics; Male; Microvilli; Osmolar Concentration; Punctures; Rats; Rats, Wistar; Sodium-Hydrogen Antiporter; Time Factors
Año:2001
Volumen:280
Número:6 49-6
Página de inicio:R1627
Página de fin:R1631
Título revista:American Journal of Physiology - Regulatory Integrative and Comparative Physiology
Título revista abreviado:Am. J. Physiol. Regul. Integr. Comp. Physiol.
ISSN:03636119
CODEN:AJPRD
CAS:Acids; Bicarbonates; Sodium-Hydrogen Antiporter
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03636119_v280_n649-6_pR1627_MacLaughlin

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Citas:

---------- APA ----------
Mac Laughlin, M., Damasco, M.C., Igarreta, P. & Amorena, C. (2001) . In vitro and in vivo evaluation of proximal tubular acidification in aging rats. American Journal of Physiology - Regulatory Integrative and Comparative Physiology, 280(6 49-6), R1627-R1631.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03636119_v280_n649-6_pR1627_MacLaughlin [ ]
---------- CHICAGO ----------
Mac Laughlin, M., Damasco, M.C., Igarreta, P., Amorena, C. "In vitro and in vivo evaluation of proximal tubular acidification in aging rats" . American Journal of Physiology - Regulatory Integrative and Comparative Physiology 280, no. 6 49-6 (2001) : R1627-R1631.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03636119_v280_n649-6_pR1627_MacLaughlin [ ]
---------- MLA ----------
Mac Laughlin, M., Damasco, M.C., Igarreta, P., Amorena, C. "In vitro and in vivo evaluation of proximal tubular acidification in aging rats" . American Journal of Physiology - Regulatory Integrative and Comparative Physiology, vol. 280, no. 6 49-6, 2001, pp. R1627-R1631.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03636119_v280_n649-6_pR1627_MacLaughlin [ ]
---------- VANCOUVER ----------
Mac Laughlin, M., Damasco, M.C., Igarreta, P., Amorena, C. In vitro and in vivo evaluation of proximal tubular acidification in aging rats. Am. J. Physiol. Regul. Integr. Comp. Physiol. 2001;280(6 49-6):R1627-R1631.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03636119_v280_n649-6_pR1627_MacLaughlin [ ]