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Abstract:

The effect of nisin combined with pulsed electric fields (PEF) and water activity reduction by sodium chloride (NaCl) on the inactivation of E. coli in simulated milk ultrafiltrate media was studied with a Doehlert design and a response surface method. The reduction of water activity from 0.99 to 0.95 by the addition of NaCl (without any other hurdle) did not affect E. coli viability of approximately 108 CFU/ml. A reduction in PEF effectiveness occurred when the NaCl concentration was increased because of an increase in conductance, which reduced the pulse decay time. In cells submitted to PEF, nisin activity was decreased, probably as a consequence of the nonspecific binding of nisin to cellular debris or the emergence of new binding sites in or from cells. However, the lethal effect due to nisin was reestablished and further improved when water activity was reduced to 0.95. A synergistic effect was evidenced when low-intensity PEF were applied. Decreasing water activity to 0.95 and applying PEF at 5 kV/cm (a nonlethal intensity when no other hurdle is used) with the further addition of nisin (1,200 IU/ml) resulted in a 5-log cycle reduction of the bacterial population.

Registro:

Documento: Artículo
Título:Inactivation of Escherichia coli by a combination of nisin, pulsed electric fields, and water activity reduction by sodium chloride
Autor:Terebiznik, M.; Jagus, R.; Cerrutti, P.; De Huergo, M.S.; Pilosof, A.M.R.
Filiación:Departamento de Ingeniería Química, Universidad de Buenos Aires, Ciudad Universitaria, 1428, Buenos Aires, Argentina
Departamento de Ingenieria Industrial, Facultad de Ingeneria, Ciudad Universitaria, 1428, Buenos Aires, Argentina
Departamento de Industrias, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, 1428, Buenos Aires, Argentina
Palabras clave:Bacteria (microorganisms); Escherichia coli; Escherichia coli
Año:2002
Volumen:65
Número:8
Página de inicio:1253
Página de fin:1258
DOI: http://dx.doi.org/10.4315/0362-028X-65.8.1253
Título revista:Journal of Food Protection
Título revista abreviado:J. Food Protection
ISSN:0362028X
CODEN:JFPRD
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0362028X_v65_n8_p1253_Terebiznik

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Citas:

---------- APA ----------
Terebiznik, M., Jagus, R., Cerrutti, P., De Huergo, M.S. & Pilosof, A.M.R. (2002) . Inactivation of Escherichia coli by a combination of nisin, pulsed electric fields, and water activity reduction by sodium chloride. Journal of Food Protection, 65(8), 1253-1258.
http://dx.doi.org/10.4315/0362-028X-65.8.1253
---------- CHICAGO ----------
Terebiznik, M., Jagus, R., Cerrutti, P., De Huergo, M.S., Pilosof, A.M.R. "Inactivation of Escherichia coli by a combination of nisin, pulsed electric fields, and water activity reduction by sodium chloride" . Journal of Food Protection 65, no. 8 (2002) : 1253-1258.
http://dx.doi.org/10.4315/0362-028X-65.8.1253
---------- MLA ----------
Terebiznik, M., Jagus, R., Cerrutti, P., De Huergo, M.S., Pilosof, A.M.R. "Inactivation of Escherichia coli by a combination of nisin, pulsed electric fields, and water activity reduction by sodium chloride" . Journal of Food Protection, vol. 65, no. 8, 2002, pp. 1253-1258.
http://dx.doi.org/10.4315/0362-028X-65.8.1253
---------- VANCOUVER ----------
Terebiznik, M., Jagus, R., Cerrutti, P., De Huergo, M.S., Pilosof, A.M.R. Inactivation of Escherichia coli by a combination of nisin, pulsed electric fields, and water activity reduction by sodium chloride. J. Food Protection. 2002;65(8):1253-1258.
http://dx.doi.org/10.4315/0362-028X-65.8.1253