Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

The Doehlert design was applied in order to investigate the combined effect of nisin and high voltage pulsed electric fields (PEF) on the inactivation of Escherichia coli in simulated milk ultrafiltrate media. Nisin alone was totally inactivated by PEF, but in the presence of bacterial cells a protective effect was observed. However, the effectiveness of nisin was still decreased when bacterial cells were subjected to the combined treatment. In spite of this phenomenon, an almost additive response emerged as a consequence of the combined treatment. A 4-log cycle reduction may be accomplished with around 1,000 IU/ml (7.15 μM) of nisin and three pulses of 11.25 kV/cm or 500 IU/ml for five pulses of the same intensity. The observed efficacy arising from the combination of both treatments suggests the possibility of using PEF for improving the action spectrum of natural antimicrobials.

Registro:

Documento: Artículo
Título:Combined effect of nisin and pulsed electric fields on the inactivation of Escherichia coli
Autor:Terebiznik, M.R.; Jagus, R.J.; Cerrutti, P.; De Huergo, M.S.; Pilosof, A.M.R.
Filiación:Departamento de Ing. Química, Universidad de Buenos Aires, Ciudad Universitaria (1428), Buenos Aires, Argentina
Depto. de Ing. Industrial, Universidad de Buenos Aires, Ciudad Universitaria (1428), Buenos Aires, Argentina
Departamento de Industrias, Universidad de Buenos Aires, Ciudad Universitaria (1428), Buenos Aires, Argentina
Palabras clave:antibacterial activity; bacterium culture; culture medium; Escherichia coli; nisin; pulsed electric field; simulation; Bacteria (microorganisms); Escherichia coli
Año:2000
Volumen:63
Número:6
Página de inicio:741
Página de fin:746
DOI: http://dx.doi.org/10.4315/0362-028X-63.6.741
Título revista:Journal of Food Protection
Título revista abreviado:J. Food Protection
ISSN:0362028X
CODEN:JFPRD
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0362028X_v63_n6_p741_Terebiznik

Referencias:

  • Abee, T., Pore-forming bacteriocins of gram-positive bacteria and self-protection mechanisms of producer organisms (1995) FEMS Microbiol. Lett., 129, pp. 1-10
  • Barbosa-Cànovas, G.V., Pothakamury, U.R., Palou, E., Swanson, B.G., Biological effects and applications of pulsed electric fields for the preservation of foods (1998) Nonthermal Preservation of Foods, pp. 73-112. , O. R. Fennema, M. Karel, G. W. Sanderson, S. R. Tannenbaum, P. Walstra, and J. R. Whitaker (ed.). Marcel Dekker, Inc., New York
  • Barsotti, L., Cheftel, J.C., Food processing by pulsed electric fields. II. Biological aspects (1999) Food Rev. Int., 15, pp. 181-213
  • Bruno, M.E., Kaiser, A., Montville, T.J., Depletion of proton motive force by nisin in Listeria monocytogenes cells (1992) Appl. Environ. Microbiol., 58, pp. 2255-2259
  • Carneiro De Melo, A., Cook, G., Miles, R., Poole, R., Nisin stimulates oxygen consumption by Staphylococcus aureus and escherichia coli (1996) Appl. Environ. Microbiol., 62, pp. 1831-1834
  • Cutter, C.N., Siragusa, G.R., Incorporation of nisin into a meat binding system to inhibit bacteria on beef surfaces (1998) Lett. Appl. Microbiol., 27, pp. 19-23
  • Doehlert, D.H., Uniform shell designs (1970) Appl. Stat., 19, pp. 231-239
  • Driessen, A.J.M., Van Den Hooven, H.W., Kuiper, W., Van De Kamp, M., Sahl, H.G., Konings, R.N.H., Konings, W.N., Mechanistic studies of lantibiotic-induced permeabilization of phospholipid vesicles (1995) Biochemistry, 34, pp. 1606-1614
  • Garcia Garcerá, M.J., Elferink, M.G.L., Driessen, A.J.M., Konings, W.N., In vitro pore-forming activity of the lantibiotic nisin (1993) Eur. J. Biochem., 212, pp. 417-422
  • Hansen, J.N., Nisin as a model food preservative (1994) Crit. Rev. Food Sci. Nutr., 34, pp. 69-93
  • Hauben, K.J.A., Wuytack, E.Y., Soontjens, C.C.F., Michiels, C.W., High pressure transient sensitization of E. coli to lysozyme and nisin by disruption of outer membrane permeability (1996) J. Food Prot., 59, pp. 350-355
  • Kalchayanand, N., Hanlin, M.B., Ray, B., Sublethal injury makes gram-negative and resistant gram-positive bacteria sensitive to the bacteriocins, pediocins AcH and nisin (1992) Lett. Appl. Microbiol., 15, pp. 239-243
  • Kalchayanand, N., Sikes, T., Dunne, C.P., Ray, B., Hydrostatic pressure and electroporation have increased bactericidal efficiency in combination with bacteriocins (1994) Appl. Environ. Microbiol., 60, pp. 4174-4177
  • Leistner, L., Gorris, L.G.M., Food preservation by hurdle technology (1995) Trends Food Sci. Technol., 6, pp. 41-46
  • Moll, G.N., Clark, J., Chan, W.C., Bycroft, B.W., Roberts, G.C.K., Konings, W.N., Driessen, A.J.M., Role of transmembrane pH gradient and membrane binding in nisin pore formation (1997) J. Bacteriol., 179, pp. 135-140
  • Nettles, C.G., Barefoot, S.F., Biochemical and genetic characteristics of bacteriocins of food-associated lactic acid bacteria (1993) J. Food Prot., 56, pp. 338-356
  • Nikaido, H., Vaara, M., Molecular basis of bacterial outer membrane permeability (1985) Microbiol. Rev., 49, pp. 1-32
  • Pothakamury, U.R., Monsalve-Gonzalez, A., Barbosa-Cànovas, G.V., Swanson, B.G., Inactivation of Escherichia coli and Staphylococcus aureus in model foods by pulsed electric field technology (1995) Food Res. Int., 28, pp. 167-171
  • Rollema, H.S., Kuipers, O.P., Both, P., De Vos, W.M., Siezen, R.J., Improvement of solubility and stability of the anti-microbial peptide nisin by protein engineering (1995) Appl. Environ. Microbiol., 61, pp. 2873-2878
  • Rossano, R., Fiore, A.D., D'Elia, A., Pesole, G., Parente, E., Riccio, P., New procedure for the determination of nisin in milk (1998) Biotechnol. Tech., 10, pp. 783-786
  • Simpson, R.K., Whittington, R., Earnshaw, R.G., Russell, J., Pulsed high electric field causes "all or nothing" membrane damage in Listeria monocytogenes and Salmonella ryphimurium, but membrane H'-ATpase is not a primary target (1999) Int. J. Food Microbiol., 48, pp. 1-10
  • Wei, L., Hansen, N., Some chemical and physical properties of nisin, a small-protein antibiotic produced by Lactococcus lactis (1990) Appl. Environ. Microbiol., 56, pp. 2551-2558
  • Zapico, P., De Paz, M., Medina, M., Nuñz, M., The effect of homogenization of whole milk, skim milk and milk fat on nisin activity against Listeria innocua (1999) Int. J. Food Microbiol., 46, pp. 151-157

Citas:

---------- APA ----------
Terebiznik, M.R., Jagus, R.J., Cerrutti, P., De Huergo, M.S. & Pilosof, A.M.R. (2000) . Combined effect of nisin and pulsed electric fields on the inactivation of Escherichia coli. Journal of Food Protection, 63(6), 741-746.
http://dx.doi.org/10.4315/0362-028X-63.6.741
---------- CHICAGO ----------
Terebiznik, M.R., Jagus, R.J., Cerrutti, P., De Huergo, M.S., Pilosof, A.M.R. "Combined effect of nisin and pulsed electric fields on the inactivation of Escherichia coli" . Journal of Food Protection 63, no. 6 (2000) : 741-746.
http://dx.doi.org/10.4315/0362-028X-63.6.741
---------- MLA ----------
Terebiznik, M.R., Jagus, R.J., Cerrutti, P., De Huergo, M.S., Pilosof, A.M.R. "Combined effect of nisin and pulsed electric fields on the inactivation of Escherichia coli" . Journal of Food Protection, vol. 63, no. 6, 2000, pp. 741-746.
http://dx.doi.org/10.4315/0362-028X-63.6.741
---------- VANCOUVER ----------
Terebiznik, M.R., Jagus, R.J., Cerrutti, P., De Huergo, M.S., Pilosof, A.M.R. Combined effect of nisin and pulsed electric fields on the inactivation of Escherichia coli. J. Food Protection. 2000;63(6):741-746.
http://dx.doi.org/10.4315/0362-028X-63.6.741