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Abstract:

Rab31, a protein that we cloned from an oligodendrocyte cDNA library, is required for transport of mannose 6-phosphate receptors (MPRs) from the trans-Golgi network (TGN) to endosomes and for Golgi/TGN organization. Here we extend the knowledge of the mechanism of action of Rab31 by demonstrating its interaction with OCRL-1, a phosphatidylinositol 4,5-diphosphate 5-phosphatase (PI(4,5)P2 5-phosphatase) that regulates the levels of PI(4,5)P 2 and PI(4)P, molecules involved in transport and Golgi/TGN organization. We show that Rab31 interacts with OCRL-1 in a yeast two-hybrid system, GST-Rab31 pull-down experiments, and coimmunoprecipitation of OCRL-1 using oligodendrocyte culture lysates. Rab31 and OCRL-1 colocalize in the TGN, post-TGN carriers, and endosomes. Cation-dependent MPR (CD-MPR) is sorted to OCRL-1-containing carriers, but CD63 and vesicular stomatitis virus G (VSVG) are not. siRNA-mediated depletion of endogenous Rab31 causes collapse of the TGN apparatus and markedly decreases the levels of OCRL-1 in the TGN and endosomes. Our observations indicate that the role of Rab31 in the Golgi/TGN structure and transport of MPRs depends on its capability to recruit OCRL-1 to domains of the TGN where the formation of carriers occurs. The importance of our observations is highlighted by the fact that mutation of OCRL-1 causes demyelination in humans. © 2009 Wiley-Liss, Inc.

Registro:

Documento: Artículo
Título:Interaction of Rab31 and OCRL-1 in oligodendrocytes: Its role in transport of mannose 6-phosphate receptors
Autor:Rodriguez-Gabin, A.G.; Ortiz, E.; Demoliner, K.; Si, Q.; Almazan, G.; Larocca, J.N.
Filiación:Department of Neurology, Albert Einstein College of Medicine, Yeshiva University, Bronx, NY, United States
Departamento de Química Biológica, Instituto de Química Y Fisicoquímica Biológica, UBA-CONICET, Buenos Aires, Argentina
Department of Pharmacology and Therapeutics, McGill University, Montreal, QC, Canada
Department of Neuroscience, Albert Einstein College of Medicine, Yeshiva University, Bronx, NY, United States
Palabras clave:Endosomes; Myelin; Oligodendrocytes; Phosphatidylinositol 4,5-diphosphate 5-phosphatase; Rab proteins; Signaling; Trans-Golgi network; Vesicle transport; cation; CD36 antigen; phosphatase; phosphatidylinositol 4,5 diphosphate 5 phosphatase; Rab protein; small interfering RNA; somatomedin B receptor; unclassified drug; animal cell; animal tissue; article; cell lysate; controlled study; demyelination; endosome; experiment; female; Golgi complex; heterozygote detection; human; human cell; immunoprecipitation; mutation; nerve cell culture; nonhuman; oligodendroglia; priority journal; protein interaction; protein protein interaction; rat; stomatitis; trans Golgi network; two hybrid system; Vesicular stomatitis virus; yeast; Animals; Antigens, CD; Brain; Cations; Cell Membrane; Cells, Cultured; Endosomes; Hela Cells; Humans; Mice; Oligodendroglia; Phosphoric Monoester Hydrolases; Platelet Membrane Glycoproteins; Protein Binding; Protein Conformation; rab GTP-Binding Proteins; Rats; Receptor, IGF Type 2; trans-Golgi Network
Año:2010
Volumen:88
Número:3
Página de inicio:589
Página de fin:604
DOI: http://dx.doi.org/10.1002/jnr.22236
Título revista:Journal of Neuroscience Research
Título revista abreviado:J. Neurosci. Res.
ISSN:03604012
CODEN:JNRED
CAS:phosphatase, 9013-05-2; Antigens, CD; Cations; Cd63 protein, rat; Phosphoric Monoester Hydrolases, 3.1.3.-; Platelet Membrane Glycoproteins; Receptor, IGF Type 2; lysosomal protein GP53; rab GTP-Binding Proteins, 3.6.1.-
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03604012_v88_n3_p589_RodriguezGabin

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Citas:

---------- APA ----------
Rodriguez-Gabin, A.G., Ortiz, E., Demoliner, K., Si, Q., Almazan, G. & Larocca, J.N. (2010) . Interaction of Rab31 and OCRL-1 in oligodendrocytes: Its role in transport of mannose 6-phosphate receptors. Journal of Neuroscience Research, 88(3), 589-604.
http://dx.doi.org/10.1002/jnr.22236
---------- CHICAGO ----------
Rodriguez-Gabin, A.G., Ortiz, E., Demoliner, K., Si, Q., Almazan, G., Larocca, J.N. "Interaction of Rab31 and OCRL-1 in oligodendrocytes: Its role in transport of mannose 6-phosphate receptors" . Journal of Neuroscience Research 88, no. 3 (2010) : 589-604.
http://dx.doi.org/10.1002/jnr.22236
---------- MLA ----------
Rodriguez-Gabin, A.G., Ortiz, E., Demoliner, K., Si, Q., Almazan, G., Larocca, J.N. "Interaction of Rab31 and OCRL-1 in oligodendrocytes: Its role in transport of mannose 6-phosphate receptors" . Journal of Neuroscience Research, vol. 88, no. 3, 2010, pp. 589-604.
http://dx.doi.org/10.1002/jnr.22236
---------- VANCOUVER ----------
Rodriguez-Gabin, A.G., Ortiz, E., Demoliner, K., Si, Q., Almazan, G., Larocca, J.N. Interaction of Rab31 and OCRL-1 in oligodendrocytes: Its role in transport of mannose 6-phosphate receptors. J. Neurosci. Res. 2010;88(3):589-604.
http://dx.doi.org/10.1002/jnr.22236