Artículo

Badaracco, M.E.; Ortiz, E.H.; Soto, E.F.; Connor, J.; Pasquini, J.M. "Effect of transferrin on hypomyelination induced by iron deficiency" (2008) Journal of Neuroscience Research. 86(12):2663-2673
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Abstract:

We have used a model of iron deficiency in the rat to analyze the effects of a disruption in iron availability on oligodendroglial cell (OLGc) maturation and myelinogenesis and to explore the possible beneficial influence of an intracranial injection (ICI) of apotransferrin (aTf) at 3 days of age on this process. Studies carried out on postnatal days 17 and 24 showed that iron deficiency produced a decrease in myelin proteins and lipids at 24 days of age. Immunohistochemistry showed that in untreated iron-deficient (ID) rats, the immunoreactivity of anti-adenomatous polyposis coli (APC) and anti-MBP antibodies decreased markedly with reference to normal controls, whereas in ID rats treated with an ICI of aTf, the immunoreactivity of these markers increased. A similar situation occurred with the immunoreactivity of H-ferritin. In primary OLGc cultures from ID rats, there was a high number of cells positive to the antibody against the polysialylated form of the cell surface glycoprotein NCAM (PSA-NCAM) compared with in OLGc cultures prepared from normal controls or from ID animals treated with aTf. The number of MBP+ cells in cultures from ID rats increased after treatment with aTf. The presence of lipid rafts evaluated with a specific anti-protein prion cellular (PrPc) antibody showed a smaller number of PrPc-positive structures in ID rat cultures. Treatment of the ID animals with a single ICI of aTf stimulated myelination, producing a significant correction in the different biochemical parameters affected by ID. © 2008 Wiley-Liss, Inc.

Registro:

Documento: Artículo
Título:Effect of transferrin on hypomyelination induced by iron deficiency
Autor:Badaracco, M.E.; Ortiz, E.H.; Soto, E.F.; Connor, J.; Pasquini, J.M.
Filiación:Instituto de Química y Fisicoquímica Biológica (IQUIFIB), UBA-CONICET, Departamento de Química Biológica, Junín 956, Buenos Aires C1113AAD, Argentina
Hershey Medical School, Hershey, PA, United States
Palabras clave:Ferritin; Hypomyelination; Iron deficiency; Oligodendroglial cells; Transferrin; APC protein; apotransferrin; ferritin; galactolipid; myelin basic protein; myelin protein; nerve cell adhesion molecule; phospholipid; prion protein antibody; animal experiment; animal model; animal tissue; article; brain weight; cell maturation; controlled study; immunocytochemistry; immunohistochemistry; iron deficiency; iron restriction; lipid raft; myelin sheath; myelination; nonhuman; oligodendroglia; priority journal; rat; remyelinization; Anemia, Iron-Deficiency; Animals; Animals, Newborn; Apoproteins; Cells, Cultured; Demyelinating Diseases; Disease Models, Animal; Female; Myelin Sheath; Nerve Fibers, Myelinated; Pregnancy; Rats; Rats, Wistar; Transferrin
Año:2008
Volumen:86
Número:12
Página de inicio:2663
Página de fin:2673
DOI: http://dx.doi.org/10.1002/jnr.21709
Título revista:Journal of Neuroscience Research
Título revista abreviado:J. Neurosci. Res.
ISSN:03604012
CODEN:JNRED
CAS:ferritin, 9007-73-2; Apoproteins; apotransferrin; Transferrin, 11096-37-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03604012_v86_n12_p2663_Badaracco

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Citas:

---------- APA ----------
Badaracco, M.E., Ortiz, E.H., Soto, E.F., Connor, J. & Pasquini, J.M. (2008) . Effect of transferrin on hypomyelination induced by iron deficiency. Journal of Neuroscience Research, 86(12), 2663-2673.
http://dx.doi.org/10.1002/jnr.21709
---------- CHICAGO ----------
Badaracco, M.E., Ortiz, E.H., Soto, E.F., Connor, J., Pasquini, J.M. "Effect of transferrin on hypomyelination induced by iron deficiency" . Journal of Neuroscience Research 86, no. 12 (2008) : 2663-2673.
http://dx.doi.org/10.1002/jnr.21709
---------- MLA ----------
Badaracco, M.E., Ortiz, E.H., Soto, E.F., Connor, J., Pasquini, J.M. "Effect of transferrin on hypomyelination induced by iron deficiency" . Journal of Neuroscience Research, vol. 86, no. 12, 2008, pp. 2663-2673.
http://dx.doi.org/10.1002/jnr.21709
---------- VANCOUVER ----------
Badaracco, M.E., Ortiz, E.H., Soto, E.F., Connor, J., Pasquini, J.M. Effect of transferrin on hypomyelination induced by iron deficiency. J. Neurosci. Res. 2008;86(12):2663-2673.
http://dx.doi.org/10.1002/jnr.21709