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Abstract:

The targeting of polypeptides to restricted cytoplasmic domains by means of mRNA sorting is a widespread phenomena utilized by many cell types. In the central nervous system, in situ hybridization analysis has shown previously that the mRNAs encoding several myelin-specific proteins are specifically located within the myelinating processes of oligodendrocytes. Here, by means of biochemical and subcellular fractionation methods, we show that a myelin fraction is selectively enriched in those mRNAs. The four major myelin basic protein (MBP) mRNAs that arise by alternative splicing of exons II and VI of the MBP gene are concentrated in this subcellular fraction. Furthermore, an interaction of MBP and MOBP 81A mRNAs with the cytoskeleton was observed. This interaction might serve to mediate the anchoring of these messengers after translocation to the subcellular site of translation.

Registro:

Documento: Artículo
Título:Subcellular fractionation and association with the cytoskeleton of messengers encoding myelin proteins
Autor:Boccaccio, G.L.; Carminatti, H.; Colman, D.R.
Filiación:Inst. Invest. Bioquimicas Fund. C., Fac. de Ciencias Exactas y Naturales, UBA, Buenos Aires, Argentina
Brookdale Ctr. for Molecular Biology, Mount Sinai School of Medicine, New York, NY, United States
Inst. Invest. Bioquimicas Fund. C., Fac. de Ciencias Exactas y Naturales, UBA, Antonio Machado 151 (1405), Buenos Aires, Argentina
Palabras clave:MBP mRNA; MOBP mRNA; MRNA transport; myelin basic protein; RNA; animal cell; article; cell fractionation; cytoskeleton; in situ hybridization; mouse; myelin sheath; nonhuman; priority journal; protein determination; protein targeting; RNA analysis; RNA splicing; RNA translation; RNA transport; Alternative Splicing; Animals; Blotting, Northern; Blotting, Western; Cell Membrane; Cytoskeleton; Exons; Immunoblotting; Mice; Mice, Inbred C57BL; Myelin Proteins; Myelin Sheath; Oligodendroglia; Osmotic Pressure; Plasmids; Reverse Transcriptase Polymerase Chain Reaction; RNA Probes; RNA, Messenger; Subcellular Fractions
Año:1999
Volumen:58
Número:4
Página de inicio:480
Página de fin:491
DOI: http://dx.doi.org/10.1002/(SICI)1097-4547(19991115)58:4<480::AID-JNR2>3.0.CO;2-Z
Título revista:Journal of Neuroscience Research
Título revista abreviado:J. Neurosci. Res.
ISSN:03604012
CODEN:JNRED
CAS:Myelin Proteins; RNA Probes; RNA, Messenger
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03604012_v58_n4_p480_Boccaccio

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Citas:

---------- APA ----------
Boccaccio, G.L., Carminatti, H. & Colman, D.R. (1999) . Subcellular fractionation and association with the cytoskeleton of messengers encoding myelin proteins. Journal of Neuroscience Research, 58(4), 480-491.
http://dx.doi.org/10.1002/(SICI)1097-4547(19991115)58:4<480::AID-JNR2>3.0.CO;2-Z
---------- CHICAGO ----------
Boccaccio, G.L., Carminatti, H., Colman, D.R. "Subcellular fractionation and association with the cytoskeleton of messengers encoding myelin proteins" . Journal of Neuroscience Research 58, no. 4 (1999) : 480-491.
http://dx.doi.org/10.1002/(SICI)1097-4547(19991115)58:4<480::AID-JNR2>3.0.CO;2-Z
---------- MLA ----------
Boccaccio, G.L., Carminatti, H., Colman, D.R. "Subcellular fractionation and association with the cytoskeleton of messengers encoding myelin proteins" . Journal of Neuroscience Research, vol. 58, no. 4, 1999, pp. 480-491.
http://dx.doi.org/10.1002/(SICI)1097-4547(19991115)58:4<480::AID-JNR2>3.0.CO;2-Z
---------- VANCOUVER ----------
Boccaccio, G.L., Carminatti, H., Colman, D.R. Subcellular fractionation and association with the cytoskeleton of messengers encoding myelin proteins. J. Neurosci. Res. 1999;58(4):480-491.
http://dx.doi.org/10.1002/(SICI)1097-4547(19991115)58:4<480::AID-JNR2>3.0.CO;2-Z