Artículo
Ibañez, I.L.; Bracalente, C.; Molinari, B.L.; Palmieri, M.A.; Policastro, L.; Kreiner, A.J.; Burlón, A.A.; Valda, A.; Navalesi, D.; Davidson, J.; Davidson, M.; Vázquez, M.; Ozafrán, M.; Durán, H. "Induction and Rejoining of DNA Double Strand Breaks Assessed by H2AX Phosphorylation in Melanoma Cells Irradiated with Proton and Lithium Beams" (2009) International Journal of Radiation Oncology Biology Physics. 74(4):1226-1235
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Abstract:
Purpose: The aim of this study was to evaluate the induction and rejoining of DNA double strand breaks (DSBs) in melanoma cells exposed to low and high linear energy transfer (LET) radiation. Methods and Materials: DSBs and survival were determined as a function of dose in melanoma cells (B16-F0) irradiated with monoenergetic proton and lithium beams and with a gamma source. Survival curves were obtained by clonogenic assay and fitted to the linear-quadratic model. DSBs were evaluated by the detection of phosphorylated histone H2AX (γH2AX) foci at 30 min and 6 h post-irradiation. Results: Survival curves showed the increasing effectiveness of radiation as a function of LET. γH2AX labeling showed an increase in the number of foci vs. dose for all the radiations evaluated. A decrease in the number of foci was found at 6 h post-irradiation for low LET radiation, revealing the repair capacity of DSBs. An increase in the size of γH2AX foci in cells irradiated with lithium beams was found, as compared with gamma and proton irradiations, which could be attributed to the clusters of DSBs induced by high LET radiation. Foci size increased at 6 h post-irradiation for lithium and proton irradiations in relation with persistent DSBs, showing a correlation with surviving fraction. Conclusions: Our results showed the response of B16-F0 cells to charged particle beams evaluated by the detection of γH2AX foci. We conclude that γH2AX foci size is an accurate parameter to correlate the rejoining of DSBs induced by different LET radiations and radiosensitivity. © 2009 Elsevier Inc. All rights reserved.
Registro:
| Documento: | Artículo |
| Título: | Induction and Rejoining of DNA Double Strand Breaks Assessed by H2AX Phosphorylation in Melanoma Cells Irradiated with Proton and Lithium Beams |
| Autor: | Ibañez, I.L.; Bracalente, C.; Molinari, B.L.; Palmieri, M.A.; Policastro, L.; Kreiner, A.J.; Burlón, A.A.; Valda, A.; Navalesi, D.; Davidson, J.; Davidson, M.; Vázquez, M.; Ozafrán, M.; Durán, H. |
| Filiación: | Comisión Nacional de Energía Atómica, San Martín, Argentina Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina Escuela de Ciencia y Tecnología, Universidad Nacional de San Martín, Argentina |
| Palabras clave: | γH2AX; DNA DSB; Ionizing radiation; LET; Melanoma cells; Clonogenic assays; DNA double strand breaks; DNA DSB; Gamma source; High linear energy transfers; In-cell; LET; LET radiation; Linear-quadratic models; Low-LET radiation; Melanoma cells; Monoenergetic protons; Phosphorylated histone; Postirradiation; Survival curves; Surviving fractions; Cell membranes; DNA; Energy transfer; Genes; Ionizing radiation; Lithium; Nucleic acids; Phosphorylation; Proton irradiation; Protons; Radiation shielding; Radioactivity; Lighting fixtures; double stranded DNA; histone H2AX; animal cell; article; cancer cell culture; cancer radiotherapy; cancer survival; clonogenic assay; DNA determination; DNA strand breakage; linear energy transfer; low energy radiation; melanoma; melanoma cell; mouse; nonhuman; priority journal; protein determination; protein phosphorylation; proton radiation; radiation dose; Biological Markers; Cell Survival; DNA Breaks, Double-Stranded; DNA Repair; Dose-Response Relationship, Radiation; Histones; Humans; Linear Energy Transfer; Lithium; Melanoma, Experimental; Phosphorylation; Protons; Radiation Tolerance; Radioisotopes |
| Año: | 2009 |
| Volumen: | 74 |
| Número: | 4 |
| Página de inicio: | 1226 |
| Página de fin: | 1235 |
| DOI: | http://dx.doi.org/10.1016/j.ijrobp.2009.02.070 |
| Título revista: | International Journal of Radiation Oncology Biology Physics |
| Título revista abreviado: | Int. J. Radiat. Oncol. Biol. Phys. |
| ISSN: | 03603016 |
| CODEN: | IOBPD |
| CAS: | Biological Markers; H2AFX protein, human; Histones; Lithium, 7439-93-2; Protons; Radioisotopes |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03603016_v74_n4_p1226_Ibanez |
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Citas:
---------- APA ----------
Ibañez, I.L., Bracalente, C., Molinari, B.L., Palmieri, M.A., Policastro, L., Kreiner, A.J., Burlón, A.A.,..., Durán, H. (2009) . Induction and Rejoining of DNA Double Strand Breaks Assessed by H2AX Phosphorylation in Melanoma Cells Irradiated with Proton and Lithium Beams. International Journal of Radiation Oncology Biology Physics, 74(4), 1226-1235.http://dx.doi.org/10.1016/j.ijrobp.2009.02.070
---------- CHICAGO ----------
Ibañez, I.L., Bracalente, C., Molinari, B.L., Palmieri, M.A., Policastro, L., Kreiner, A.J., et al. "Induction and Rejoining of DNA Double Strand Breaks Assessed by H2AX Phosphorylation in Melanoma Cells Irradiated with Proton and Lithium Beams" . International Journal of Radiation Oncology Biology Physics 74, no. 4 (2009) : 1226-1235.http://dx.doi.org/10.1016/j.ijrobp.2009.02.070
---------- MLA ----------
Ibañez, I.L., Bracalente, C., Molinari, B.L., Palmieri, M.A., Policastro, L., Kreiner, A.J., et al. "Induction and Rejoining of DNA Double Strand Breaks Assessed by H2AX Phosphorylation in Melanoma Cells Irradiated with Proton and Lithium Beams" . International Journal of Radiation Oncology Biology Physics, vol. 74, no. 4, 2009, pp. 1226-1235.http://dx.doi.org/10.1016/j.ijrobp.2009.02.070
---------- VANCOUVER ----------
Ibañez, I.L., Bracalente, C., Molinari, B.L., Palmieri, M.A., Policastro, L., Kreiner, A.J., et al. Induction and Rejoining of DNA Double Strand Breaks Assessed by H2AX Phosphorylation in Melanoma Cells Irradiated with Proton and Lithium Beams. Int. J. Radiat. Oncol. Biol. Phys. 2009;74(4):1226-1235.http://dx.doi.org/10.1016/j.ijrobp.2009.02.070
