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Petrocelli, S.; Arana, M.R.; Cabrini, M.N.; Casabuono, A.C.; Moyano, L.; Beltramino, M.; Moreira, L.M.; Couto, A.S.; Orellano, E.G. "Deletion of pilA, a Minor Pilin-Like Gene, from Xanthomonas citri subsp. citri Influences Bacterial Physiology and Pathogenesis" (2016) Current Microbiology. 73(6):904-914
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Abstract:

Type IV pili (Tfp) are widely distributed adhesins of bacterial surfaces. In plant pathogenic bacteria, Tfp are involved in host colonization and pathogenesis. Xanthomonas citri subsp. citri (Xcc) is the phytopathogen responsible for citrus canker disease. In this work, three Tfp structural genes, fimA, fimA1, and pilA from Xcc were studied. A pilA mutant strain from Xcc (XccΔpilA) was constructed and differences in physiological features, such as motilities, adhesion, and biofilm formation, were observed. A structural study of the purified Tfp fractions from Xcc wild-type and Xcc∆pilA showed that pilins are glycosylated in both strains and that FimA and FimA1 are the main structural components of the pili. Furthermore, smaller lesion symptoms and reduced bacterial growth were produced by Xcc∆pilA in orange plants compared to the wild-type strain. These results indicate that the minor pilin-like gene, pilA, is involved in Tfp performance during the infection process. © 2016, Springer Science+Business Media New York.

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Documento: Artículo
Título:Deletion of pilA, a Minor Pilin-Like Gene, from Xanthomonas citri subsp. citri Influences Bacterial Physiology and Pathogenesis
Autor:Petrocelli, S.; Arana, M.R.; Cabrini, M.N.; Casabuono, A.C.; Moyano, L.; Beltramino, M.; Moreira, L.M.; Couto, A.S.; Orellano, E.G.
Filiación:Facultad de Ciencias Bioquímicas y Farmacéuticas, Instituto de Biología Molecular y Celular de Rosario (IBR), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Rosario, Suipacha 531, Rosario, S2002LRK, Argentina
Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Centro de Investigaciones en Hidratos de Carbono, Universidad de Buenos Aires, Buenos Aires, 1428, Argentina
Departamento de Ciências Biológicas (DECBI), Instituto de Ciências Exatas e Biológicas, Campus Morro do Cruzeiro, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil
Núcleo de Pesquisas em Ciências Biológicas (NUPEB), Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil
Palabras clave:bacterial protein; pilin; protein Tfp; unclassified drug; fimbria protein; Article; bacterial colonization; bacterial gene; bacterial growth; bacterial plant disease; bacterial strain; bacterial virulence; bacterium adherence; biofilm; controlled study; fimA gene; fimA1 gene; gene deletion; gene identification; gene mutation; nonhuman; pilA gene; priority journal; protein glycosylation; protein purification; structure analysis; sweet orange; Xanthomonas citri; Xanthomonas citri subsp. citri; Citrus; gene deletion; genetics; metabolism; microbiology; pathogenicity; plant disease; virulence; Xanthomonas; Bacterial Proteins; Citrus; Fimbriae Proteins; Gene Deletion; Plant Diseases; Virulence; Xanthomonas
Año:2016
Volumen:73
Número:6
Página de inicio:904
Página de fin:914
DOI: http://dx.doi.org/10.1007/s00284-016-1138-1
Título revista:Current Microbiology
Título revista abreviado:Curr. Microbiol.
ISSN:03438651
CODEN:CUMID
CAS:Bacterial Proteins; Fimbriae Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03438651_v73_n6_p904_Petrocelli

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Citas:

---------- APA ----------
Petrocelli, S., Arana, M.R., Cabrini, M.N., Casabuono, A.C., Moyano, L., Beltramino, M., Moreira, L.M.,..., Orellano, E.G. (2016) . Deletion of pilA, a Minor Pilin-Like Gene, from Xanthomonas citri subsp. citri Influences Bacterial Physiology and Pathogenesis. Current Microbiology, 73(6), 904-914.
http://dx.doi.org/10.1007/s00284-016-1138-1
---------- CHICAGO ----------
Petrocelli, S., Arana, M.R., Cabrini, M.N., Casabuono, A.C., Moyano, L., Beltramino, M., et al. "Deletion of pilA, a Minor Pilin-Like Gene, from Xanthomonas citri subsp. citri Influences Bacterial Physiology and Pathogenesis" . Current Microbiology 73, no. 6 (2016) : 904-914.
http://dx.doi.org/10.1007/s00284-016-1138-1
---------- MLA ----------
Petrocelli, S., Arana, M.R., Cabrini, M.N., Casabuono, A.C., Moyano, L., Beltramino, M., et al. "Deletion of pilA, a Minor Pilin-Like Gene, from Xanthomonas citri subsp. citri Influences Bacterial Physiology and Pathogenesis" . Current Microbiology, vol. 73, no. 6, 2016, pp. 904-914.
http://dx.doi.org/10.1007/s00284-016-1138-1
---------- VANCOUVER ----------
Petrocelli, S., Arana, M.R., Cabrini, M.N., Casabuono, A.C., Moyano, L., Beltramino, M., et al. Deletion of pilA, a Minor Pilin-Like Gene, from Xanthomonas citri subsp. citri Influences Bacterial Physiology and Pathogenesis. Curr. Microbiol. 2016;73(6):904-914.
http://dx.doi.org/10.1007/s00284-016-1138-1