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Abstract:

Planktonic cells of Sinorhizobium meliloti, a Gram-negative symbiotic bacterium, display autoaggregation under static conditions. ExpR is a LuxR-type regulator that controls many functions in S. meliloti, including synthesis of two exopolysaccharides, EPS I (succinoglycan) and EPS II (galactoglucan). Since exopolysaccharides are important for bacterial attachment, we studied the involvement of EPS I and II in autoaggregation of S. meliloti. Presence of an intact copy of the expR locus was shown to be necessary for autoaggregation. A mutant incapable of producing EPS I displayed autoaggregation percentage similar to that of parental strain, whereas autoaggregation was significantly lower for a mutant defective in biosynthesis of EPS II. Our findings clearly indicate that EPS II is the essential component involved in autoaggregation of planktonic S. meliloti cells, and that EPS I plays no role in such aggregation. © 2010 Springer Science+Business Media, LLC.

Registro:

Documento: Artículo
Título:EPS II-dependent autoaggregation of sinorhizobium meliloti planktonic cells
Autor:Sorroche, F.G.; Rinaudi, L.V.; Zorreguieta, Ã.; Giordano, W.
Filiación:Departamento de Biología Molecular, Universidad Nacional de Río Cuarto, Ruta 36 km 601, X5804BYA-Río Cuarto, Córdoba, Argentina
Fundación Instituto Leloir, IIBBA, CONICET and FCEyN, Universidad de Buenos Aires, Patricias Argentinas 435, Buenos Aires C1405BWE, Argentina
Palabras clave:exopolysaccharide; galactoglucan; mutant protein; protein expR; succinoglycan; transcription factor LuxR; unclassified drug; article; bacterial cell; bacterial strain; bacterioplankton; cell aggregation; controlled study; gene locus; nonhuman; priority journal; protein function; protein synthesis; Sinorhizobium meliloti; Biofilms; Biomass; Flocculation; Galactans; Gene Expression Regulation, Bacterial; Genes, Bacterial; Glucans; Microbial Interactions; Polysaccharides, Bacterial; Quorum Sensing; Sinorhizobium meliloti; Symbiosis; Bacteria (microorganisms); Negibacteria; Sinorhizobium meliloti
Año:2010
Volumen:61
Número:5
Página de inicio:465
Página de fin:470
DOI: http://dx.doi.org/10.1007/s00284-010-9639-9
Título revista:Current Microbiology
Título revista abreviado:Curr. Microbiol.
ISSN:03438651
CODEN:CUMID
CAS:succinoglycan, 73667-50-2; Galactans; Glucans; Polysaccharides, Bacterial; galactoglucan, 55787-15-0; succinoglycan, 73667-50-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03438651_v61_n5_p465_Sorroche

Referencias:

  • Alami, Y., Achouak, W., Marol, C., Heulin, T., Rhizosphere soil aggregation and plant growth promotion of sunflowers by an exopolysaccharide-producing Rhizobium sp. strain isolated from sunflower roots (2000) Applied and Environmental Microbiology, 66 (8), pp. 3393-3398. , DOI 10.1128/AEM.66.8.3393-3398.2000
  • Bahat-Samet, E., Castro-Sowinsky, S., Okon, Y., Arabinose content of extracellular polysaccharide plays a role in cell aggregation of Azospirillum brasilense (2004) FEMS Microbiol Lett, 237, pp. 195-203. , 1:CAS:528:DC%2BD2cXmsVGitLw%3D 10.1111/j.1574-6968.2004.tb09696.x 15321662
  • Battisti, L., Lara, J.C., Leigh, J.A., Specific oligosaccharide form of the Rhizobium meliloti exopolysaccharide promotes nodule invasion in alfalfa (1992) Proc Natl Acad Sci USA, 89, pp. 5625-5629. , 1:CAS:528:DyaK38XltVynsrk%3D 10.1073/pnas.89.12.5625 1608972
  • Beringer, J.E., R factor transfer in Rhizobium leguminosarum (1974) J Gen Microbiol, 84, pp. 188-198. , 1:STN:280:DyaE2M%2FmtFeltA%3D%3D 4612098
  • Casse, F., Boucher, C., Julliot, S., Michel, M., Dénarié, J., Identification and characterization of large plasmids in Rhizobium meliloti using agarose gel electrophoresis (1979) J Bacteriol, 113, pp. 229-242
  • Ditta, G., Stanfield, S., Corbin, D., Helinski, D.R., Broad host range DNA cloning system for gram negative bacteria: Construction of a gene bank of Rhizobium meliloti (1980) Proc Natl Acad Sci USA, 77, pp. 7347-7351
  • Finan, T.M., Hartwieg, E.K., Lemieux, K., Bergman, K., Walker, G.C., Signer, E.R., General transduction in Rhizobium meliloti (1984) J Bacteriol, 159, pp. 120-124
  • Glazebrook, J., Walker, G.C., A novel exopolysaccharide can function in place of the calcofluor-binding exopolysaccharide in nodulation of alfalfa by Rhizobium meliloti (1989) Cell, 56, pp. 661-672. , 1:CAS:528:DyaL1MXit1yhs74%3D 10.1016/0092-8674(89)90588-6 2537152
  • Glazebrook, J., Walker, G.C., Genetic techniques in Rhizobium meliloti (1991) Methods Enzymol, 204, pp. 398-418. , 1:CAS:528:DyaK38Xhs1OitLo%3D 10.1016/0076-6879(91)04021-F 1658566
  • Gonzalez, J.E., Reuhs, B.L., Walker, G.C., Low molecular weight EPS II of Rhizobium meliloti allows nodule invasion in Medicago sativa (1996) Proceedings of the National Academy of Sciences of the United States of America, 93 (16), pp. 8636-8641. , DOI 10.1073/pnas.93.16.8636
  • Her, G.R., Glazebrook, J., Walker, G.C., Reinhold, V.N., Structural studies of a novel exopolysaccharide produced by a mutant of Rhizobium meliloti strain Rm1021 (1990) Carbohydr Res, 198, pp. 305-312. , 1:CAS:528:DyaK3cXktlOntbc%3D 10.1016/0008-6215(90)84300-J 2379191
  • Hoang, H.H., Becker, A., Gonzalez, J.E., The LuxR Homolog ExpR, in combination with the sin quorum sensing system, plays a central role in Sinorhizobium meliloti gene expression (2004) Journal of Bacteriology, 186 (16), pp. 5460-5472. , DOI 10.1128/JB.186.16.5460-5472.2004
  • Kaci, Y., Heyraud, A., Barakat, M., Heulin, T., Isolation and identification of an EPS-producing Rhizobium strain from arid soil (Algeria): Characterization of its EPS and the effect of inoculation on wheat rhizosphere soil structure (2005) Research in Microbiology, 156 (4), pp. 522-531. , DOI 10.1016/j.resmic.2005.01.012
  • Keller, M., Roxlau, A., Weng, W.M., Schmidt, M., Quandt, J., Niehaus, K., Jording, D., Puhler, A., Molecular analysis of the Rhizobium meliloti mucR gene regulating the biosynthesis of the exopolysaccharides succinoglycan and galactoglucan (1995) Mol Plant Microbe Interact, 8, pp. 267-277. , 1:CAS:528:DyaK2MXls1OkurY%3D 7756693
  • Leigh, J.A., Signer, E.R., Walker, G.C., Exopolysaccharide-deficient mutants of Rhizobium meliloti that form ineffective nodules (1985) Proc Natl Acad Sci USA, 82, pp. 6231-6235
  • Matthysse, A.G., Marry, M., Krall, L., Kaye, M., Ramey, B.E., Fuqua, C., White, A.R., The effect of cellulose overproduction on binding and biofilm formation on roots by Agrobacterium tumefaciens (2005) Molecular Plant-Microbe Interactions, 18 (9), pp. 1002-1010. , DOI 10.1094/MPMI-18-1002
  • Meade, H.M., Long, S.R., Ruvkun, G.B., Brown, S.E., Ausubel, F.M., Physical and genetic characterization of symbiotic and auxotrophic mutants of Rhizobium meliloti induced by transposon Tn5 mutagenesis (1982) J Bacteriol, 149, pp. 114-122
  • Mendrygal, K.E., Gonzalez, J.E., Environmental regulation of exopolysaccharide production in Sinorhizobium meliloti (2000) Journal of Bacteriology, 182 (3), pp. 599-606. , DOI 10.1128/JB.182.3.599-606.2000
  • Morris, C.E., Monier, J.-M., The Ecological Significance of Biofilm Formation by Plant-Associated Bacteria (2003) Annual Review of Phytopathology, 41, pp. 429-453. , DOI 10.1146/annurev.phyto.41.022103.134521
  • Neyra, C., Sadasivan, L., Flocculation in Azospirillum brasilense and Azospirillum lipoferum: Exopolysacharides and Cyst Formation (1985) J Bacteriol, 163, pp. 716-723. , 3894333
  • Nikitina, V.E., Ponomareva, E.G., Alenkina, S.A., Konnova, S.A., The role of cell-surface Lectins in the aggregation of Azospirilla (2001) Microbiology, 70, pp. 471-476. , 1:STN:280:DC%2BD3MrhtFemug%3D%3D 10.1023/A:1010425925854 11558272
  • Pellock, B.J., Teplitski, M., Boinay, R.P., Bauer, W.D., Walker, G.C., A LuxR homolog controls production of symbiotically active extracellular polysaccharide II by Sinorhizobium meliloti (2002) Journal of Bacteriology, 184 (18), pp. 5067-5076. , DOI 10.1128/JB.184.18.5067-5076.2002
  • Reinhold, B.B., Chan, S.Y., Reuber, T.L., Marra, A., Walker, G.C., Reinhold, V.N., Detailed structural characterization of succinoglycan, the major exopolysaccharide of Rhizobium meliloti Rm1021 (1994) J Bacteriol, 176, pp. 1997-2002. , 1:CAS:528:DyaK2cXis1Gnt7k%3D 8144468
  • Reuber, T.L., Walker, G.C., Biosynthesis of succinoglycan, a symbiotically important exopolysaccharide of Rhizobium meliloti (1993) Cell, 74 (2), pp. 269-280. , DOI 10.1016/0092-8674(93)90418-P
  • Rinaudi, L.V., Giordano, W., An integrated view of biofilm formation in rhizobia (2010) FEMS Microbiol Lett, 304, pp. 1-11
  • Rinaudi, L.V., Gonzalez, J.E., The low-molecular-weight fraction of exopolysaccharide II from Sinorhizobium meliloti is a crucial determinant of biofilm formation (2009) J Bacteriol, 191, pp. 7216-7224. , 1:CAS:528:DC%2BD1MXhsVyrtrnM 10.1128/JB.01063-09 19783627
  • Rinaudi, L., Sorroche, F., Zorreguieta, A., Giordano, W., Analysis of mucR gene regulating biosynthesis of exopolysaccharides: Implications for biofilm formation in Sinorhizobium meliloti Rm1021 (2010) FEMS Microbiol Lett, 302, pp. 15-21. , 1:CAS:528:DC%2BD1MXhs1Wht7fF 10.1111/j.1574-6968.2009.01826.x 19929968
  • Sutherland, I.W., Biofilm exopolysaccharides: A strong and sticky framework (2001) Microbiology, 147, pp. 3-9. , 1:CAS:528:DC%2BD3MXhtlWntbk%3D 11160795
  • Lptm, Z., Succinoglycan and galactoglucan (1997) Carbohydr Polym, 33, pp. 139-144. , 1:CAS:528:DyaK1cXitVSktQ%3D%3D 10.1016/S0144-8617(97)00054-4
  • Zhan, H.J., Levery, S.B., Lee, C.C., Leigh, J.A., A second exopolysaccharide of Rhizobium meliloti strain SU47 that can function in root nodule invasion (1989) Proc Natl Acad Sci USA, 86, pp. 3055-3059. , 1:CAS:528:DyaL1MXkslCnsbk%3D 10.1073/pnas.86.9.3055 2717610
  • Zhan, H.J., Lee, C.C., Leigh, J.A., Induction of the second exopolysaccharide (EPSb) in Rhizobium meliloti SU47 by low phosphate concentrations (1991) J Bacteriol, 173, pp. 7391-7394. , 1:CAS:528:DyaK38XlsFaqsA%3D%3D 1938929

Citas:

---------- APA ----------
Sorroche, F.G., Rinaudi, L.V., Zorreguieta, Ã. & Giordano, W. (2010) . EPS II-dependent autoaggregation of sinorhizobium meliloti planktonic cells. Current Microbiology, 61(5), 465-470.
http://dx.doi.org/10.1007/s00284-010-9639-9
---------- CHICAGO ----------
Sorroche, F.G., Rinaudi, L.V., Zorreguieta, Ã., Giordano, W. "EPS II-dependent autoaggregation of sinorhizobium meliloti planktonic cells" . Current Microbiology 61, no. 5 (2010) : 465-470.
http://dx.doi.org/10.1007/s00284-010-9639-9
---------- MLA ----------
Sorroche, F.G., Rinaudi, L.V., Zorreguieta, Ã., Giordano, W. "EPS II-dependent autoaggregation of sinorhizobium meliloti planktonic cells" . Current Microbiology, vol. 61, no. 5, 2010, pp. 465-470.
http://dx.doi.org/10.1007/s00284-010-9639-9
---------- VANCOUVER ----------
Sorroche, F.G., Rinaudi, L.V., Zorreguieta, Ã., Giordano, W. EPS II-dependent autoaggregation of sinorhizobium meliloti planktonic cells. Curr. Microbiol. 2010;61(5):465-470.
http://dx.doi.org/10.1007/s00284-010-9639-9