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Abstract:

The expression of the rpoS gene during PHA depolymerization was monitored in Pseudomonas oleovorans GPol and its mutant defective in PHA degradation by analyzing the tolerance to oxidative and thermal stresses and the RpoS intracellular content. An increase in the tolerance to H2O 2 and heat shock was observed coincidentally with PHA degradation. Western blotting experiments performed in carbon-starved cultures showed that the RpoS levels were higher in the wild type than in the mutant strain. Complementation of the phaZ mutation restores the wild-type RpoS levels. These results suggest a probable association between PHA depolymerization and the stress tolerance phenotype controlled by RpoS.

Registro:

Documento: Artículo
Título:rpoS gene expression in carbon-starved cultures of the polyhydroxyalkanoate-accumulating species Pseudomonas oleovorans
Autor:Ruiz, J.A.; López, N.I.; Méndez, B.S.
Filiación:Departamento de Quimica Biologica, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428, Buenos Aires, Argentina
Palabras clave:heat shock protein; hydrogen peroxide; polyhydroxyalkanoic acid; article; bacterial gene; bacterium culture; bacterium mutant; degradation; depolymerization; gene expression; gene mutation; heat shock response; nonhuman; oxidative stress; phaZ gene; phenotype; priority journal; Pseudomonas oleovorans; RpoS gene; stress; thermal stimulation; Western blotting; Bacteria (microorganisms); Negibacteria; Pseudomonas; Pseudomonas oleovorans; Pseudomonas oleovorans
Año:2004
Volumen:48
Número:6
Página de inicio:396
Página de fin:400
DOI: http://dx.doi.org/10.1007/s00284-003-4183-5
Título revista:Current Microbiology
Título revista abreviado:Curr. Microbiol.
ISSN:03438651
CODEN:CUMID
CAS:hydrogen peroxide, 7722-84-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03438651_v48_n6_p396_Ruiz

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Citas:

---------- APA ----------
Ruiz, J.A., López, N.I. & Méndez, B.S. (2004) . rpoS gene expression in carbon-starved cultures of the polyhydroxyalkanoate-accumulating species Pseudomonas oleovorans. Current Microbiology, 48(6), 396-400.
http://dx.doi.org/10.1007/s00284-003-4183-5
---------- CHICAGO ----------
Ruiz, J.A., López, N.I., Méndez, B.S. "rpoS gene expression in carbon-starved cultures of the polyhydroxyalkanoate-accumulating species Pseudomonas oleovorans" . Current Microbiology 48, no. 6 (2004) : 396-400.
http://dx.doi.org/10.1007/s00284-003-4183-5
---------- MLA ----------
Ruiz, J.A., López, N.I., Méndez, B.S. "rpoS gene expression in carbon-starved cultures of the polyhydroxyalkanoate-accumulating species Pseudomonas oleovorans" . Current Microbiology, vol. 48, no. 6, 2004, pp. 396-400.
http://dx.doi.org/10.1007/s00284-003-4183-5
---------- VANCOUVER ----------
Ruiz, J.A., López, N.I., Méndez, B.S. rpoS gene expression in carbon-starved cultures of the polyhydroxyalkanoate-accumulating species Pseudomonas oleovorans. Curr. Microbiol. 2004;48(6):396-400.
http://dx.doi.org/10.1007/s00284-003-4183-5