Artículo

La versión final de este artículo es de uso interno de la institución.
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

The molecular analysis of a genomic region of B. megaterium revealed the presence of a gene coding for the enzyme phosphotransbutyrylase (Ptb). The enzyme activity was measured throughout the different phases of growth in B. megaterium, and its activity was found to be maximal in the late exponential growth phase. The branched amino acids isoleucine and valine activated Ptb expression. PtbBm was capable of using butyryl-CoA and 2-methyl-propionyl CoA as substrates. ActBm, a σ54 regulator from B. megaterium whose gene is situated upstream from the ptb gene, activated its expression.

Registro:

Documento: Artículo
Título:Phosphotransbutyrylase expression in Bacillus megaterium
Autor:Vazquez, G.J.; Pettinari, M.J.; Méndez, B.S.
Filiación:Departamento de Química Biológica, Ciudad Universitaria Pabellón 2, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Palabras clave:bacterial enzyme; isoleucine; phosphotransbutyrylase; unclassified drug; valine; article; Bacillus megaterium; bacterial growth; enzyme activity; enzyme substrate; gene expression regulation; nonhuman; nucleotide sequence; priority journal; protein expression; regulatory mechanism; Amino Acids, Branched-Chain; Bacillus megaterium; Culture Media; DNA-Binding Proteins; DNA-Directed RNA Polymerases; Gene Expression Regulation, Bacterial; Kinetics; Phosphate Acetyltransferase; RNA Polymerase Sigma 54; Sigma Factor; Bacillus megaterium; Bacteria (microorganisms); Posibacteria; Prokaryota
Año:2001
Volumen:42
Número:5
Página de inicio:345
Página de fin:349
DOI: http://dx.doi.org/10.1007/s002840010227
Título revista:Current Microbiology
Título revista abreviado:Curr. Microbiol.
ISSN:03438651
CODEN:CUMID
CAS:Amino Acids, Branched-Chain; Culture Media; DNA-Binding Proteins; DNA-Directed RNA Polymerases, EC 2.7.7.6; Phosphate Acetyltransferase, EC 2.3.1.8; RNA Polymerase Sigma 54, EC 2.7.7.6; Sigma Factor
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03438651_v42_n5_p345_Vazquez

Referencias:

  • Bullock, W., Fernández, J., Short, J., XL1-Blue: A high efficiency plasmid transforming recA Escherichia coli strain with β-galactosidase selection (1987) Biotechniques, 5, pp. 376-379
  • Cary, J., Petersen, D., Papoutaskis, E., Bennett, G., Cloning and expression of Clostridium acetobutylicum phosphotransbutyrylase and butyrate kinase genes in Escherichia coli (1988) J Bacteriol, 170, pp. 4613-4618
  • Debarbouille, M., Rozzen, G., Maryvonne, A., Rapoport, G., Role of BkdR, a transcriptional activator of the SigL-dependent isoleucine and valine degradation pathway in Bacillus subtilis (1999) J Bacteriol, 181, pp. 2059-2066
  • English, J., Vary, P., Isolation of recombination deficient and UV-sensitive mutants of Bacillus megaterium (1986) J Bacteriol, 165, pp. 155-160
  • Feigenbaum, J., Schultz, H., Thiolases of Escherichia coli: Purification and chain length specificities (1975) J Bacteriol, 122, pp. 407-411
  • Grundy, F., Waters, D., Tsetska, H., Henkin, T., Identification of genes involved in utilization of acetate and acetoin in B. subtillis (1993) Mol Microbiol, 10, pp. 259-271
  • Hull, R., Gill, R., Hsu, P., Minshew, P., Falkow, S., Construction and expression of recombinant plasmids encoding type 1 or D-mannose-resistant pili from urinary track infection Escherichia coli isolate (1981) Infect Immun, 33, pp. 933-938
  • Jenkins, L., Nunn, W., Regulation of the ato operon by the atoC gene in Escherichia coli (1987) J Bacteriol, 169, pp. 2096-2102
  • Jones, D., Woods, D., Acetone-butanol fermentation revisited (1986) Microbiol Rev, 50, pp. 484-524
  • Kunst, F., Ogasawara, N., Moszer, I., Albertini, A., Alloni, G., The complete genome sequence of the Gram-positive bacterium Bacillus subtilis (1997) Nature, 390, pp. 249-256
  • Lowry, O., Rosebrough, N., Farr, L., Randall, R., Protein measurement with the Folin phenol reagent (1951) J Biol Chem, 193, pp. 265-275
  • Massey, K., Schmidt, J., Brody, S., Branched-chain amino acid catabolism in bacteria (1976) Bacteriol Rev, 40, pp. 42-54
  • Pettinari, M.J., Vazquez, G.J., Kruger, N., Vary, P., Steinbüchel, A., Méndez, B.S., Trans activation of the Escherichia coli ato structural genes by a regulatory protein from Bacillus megaterium: Potential use in polyhydroxyalkanoates production (1998) Appl Microbiol Biotechnol, 49, pp. 737-742
  • Rado, T., Hoch, J., Phosphotransacetylase from Bacillus subtilis: Purification and physiological studies (1973) Biochim Biophys Acta, 321, pp. 114-125
  • Sambrook, J., Fritsch, E.F., Maniatis, T., (1989) Molecular cloning: a laboratory manual, 2nd edn., , Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, Press
  • Shingler, V., Signal sensing by σ54 dependent regulators: Derepression as a control mechanism (1996) Mol Microbiol, 19, pp. 409-416
  • Shulgovskaya, E., Pozmogova, E., Rabotnova, I., Effect of protein synthesis inhibitor chloramphenicol: Basic growth parameters of continuous and batch cultures of Bacillus megaterium (1981) Mikrobiologiya, 49, pp. 893-901
  • Trieu-Cuot, P., Carlier, C., Poyart-Salmeron, C., Courvalin, P., Shuttle vectors containing a multiple cloning site and a lacZ gene for conjugal transfer of DNA from Escherichia coli to gram positive bacteria (1991) Gene, 102, pp. 99-104
  • Vary, P., Tao, Y.P., Development of genetic methods in Bacillus megaterium (1988), 2, pp. 403-407. , Ganesan AT, Hoch JA (eds) Genetics and biotechnology of bacilli. New York: Academic Press; Vazquez, G.J., Pettinari, M.J., Méndez, B.S., (1997), EMBL nucleotide accession number: Y14588; Walter, K., Nair, R., Cary, J., Bennett, G., Papoutsakis, E., Sequence and arrangement of two genes of the butyrate-synthesis pathway of Clostridium acetobutylicum ATCC 824 (1993) Gene, 134, pp. 107-111

Citas:

---------- APA ----------
Vazquez, G.J., Pettinari, M.J. & Méndez, B.S. (2001) . Phosphotransbutyrylase expression in Bacillus megaterium. Current Microbiology, 42(5), 345-349.
http://dx.doi.org/10.1007/s002840010227
---------- CHICAGO ----------
Vazquez, G.J., Pettinari, M.J., Méndez, B.S. "Phosphotransbutyrylase expression in Bacillus megaterium" . Current Microbiology 42, no. 5 (2001) : 345-349.
http://dx.doi.org/10.1007/s002840010227
---------- MLA ----------
Vazquez, G.J., Pettinari, M.J., Méndez, B.S. "Phosphotransbutyrylase expression in Bacillus megaterium" . Current Microbiology, vol. 42, no. 5, 2001, pp. 345-349.
http://dx.doi.org/10.1007/s002840010227
---------- VANCOUVER ----------
Vazquez, G.J., Pettinari, M.J., Méndez, B.S. Phosphotransbutyrylase expression in Bacillus megaterium. Curr. Microbiol. 2001;42(5):345-349.
http://dx.doi.org/10.1007/s002840010227