Artículo
Ruzal, S.M.; Sanchez-Rivas, C. "In Bacillus subtilis DegU-P is a positive regulator of the osmotic response" (1998) Current Microbiology. 37(6):368-372
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Abstract:
The osmosensitivity presented by spoOA and degU null mutant strains of Bacillus subtilis pointed to their protein products as essential regulators for the osmotic response. This was further investigated by analyzing their transcription activity. The results showed that both spoOA-lacZ and degSU- lacZ were induced by the hypertonic medium. The actual phosphorylation state of these proteins was also analyzed by the bias of two reporter gene promoters activity (abrB-lacZ and degQ-lacZ). The absence of repression of abrB in hypertonic conditions suggested that SpoOA was not phosphorylated while the derepression of degQ promoter suggested that DegU-P was formed. These results were in accordance with the observed absence of sporulation in hyperosmotic media and semi-constitutive osmotolerance of degU(h) mutant strains known to retain phosphorylated DegU. The failure to secrete proteases and to sporulate in hypertonic media suggested that SpoOA acts through abrB regulation in the prevention of alternate responses. The role of DegU-P as positive regulator of the osmotic response seems to be settled and is discussed.
Registro:
| Documento: | Artículo |
| Título: | In Bacillus subtilis DegU-P is a positive regulator of the osmotic response |
| Autor: | Ruzal, S.M.; Sanchez-Rivas, C. |
| Filiación: | Depto. de Quim. Biológica, Pabellón II Piso 4, Ciudad Universitaria, 1428 Buenos Aires, Argentina |
| Palabras clave: | amylase; bacterial DNA; bacterial protein; beta galactosidase; hypertonic solution; proteinase; article; Bacillus subtilis; bacterial growth; bacterium mutant; bacterium transformation; catabolite repression; enzyme activity; enzyme induction; gene activity; nonhuman; osmoregulation; priority journal; promoter region; protein phosphorylation; reporter gene; sporogenesis; transcription initiation; Bacillus subtilis; Bacillus subtilis; Bacteria (microorganisms); Octodon degus; Posibacteria; Prokaryota |
| Año: | 1998 |
| Volumen: | 37 |
| Número: | 6 |
| Página de inicio: | 368 |
| Página de fin: | 372 |
| DOI: | http://dx.doi.org/10.1007/s002849900395 |
| Título revista: | Current Microbiology |
| Título revista abreviado: | Curr. Microbiol. |
| ISSN: | 03438651 |
| CODEN: | CUMID |
| CAS: | amylase, 9000-90-2, 9000-92-4, 9001-19-8; beta galactosidase; proteinase, 9001-92-7 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03438651_v37_n6_p368_Ruzal |
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Citas:
---------- APA ----------
Ruzal, S.M. & Sanchez-Rivas, C. (1998) . In Bacillus subtilis DegU-P is a positive regulator of the osmotic response. Current Microbiology, 37(6), 368-372.http://dx.doi.org/10.1007/s002849900395
---------- CHICAGO ----------
Ruzal, S.M., Sanchez-Rivas, C. "In Bacillus subtilis DegU-P is a positive regulator of the osmotic response" . Current Microbiology 37, no. 6 (1998) : 368-372.http://dx.doi.org/10.1007/s002849900395
---------- MLA ----------
Ruzal, S.M., Sanchez-Rivas, C. "In Bacillus subtilis DegU-P is a positive regulator of the osmotic response" . Current Microbiology, vol. 37, no. 6, 1998, pp. 368-372.http://dx.doi.org/10.1007/s002849900395
---------- VANCOUVER ----------
Ruzal, S.M., Sanchez-Rivas, C. In Bacillus subtilis DegU-P is a positive regulator of the osmotic response. Curr. Microbiol. 1998;37(6):368-372.http://dx.doi.org/10.1007/s002849900395
