Abstract:
Entomopathogenic bacilli B. thuringiensis israelensis (Bti) and B. sphaericus (Bf) exhibit low survival on field application. It was previously shown that their spores are very sensitive to different stress effectors (heat, UV light) and especially to osmotic variations. Since SASP (Small, Acid-Soluble Spore Proteins), α/β and γ type, are involved in spore tolerance to heat, UV light, peroxide, and salt, they were analyzed in Bti and Bf. The molecular weight, migration pattern and amino acid composition of different SASP were determined and compared with other bacilli, in particular to B. subtilis. A relation between spore osmotolerance, SASP content, and amino acid composition was shown. In addition, the absence of τ SASP in Bti and Bf is discussed.
Registro:
Documento: |
Artículo
|
Título: | SASP (small, acid-soluble spore proteins) and spore properties in Bacillus thuringiensis israelensis and Bacillus sphaericus |
Autor: | Cucchi, A.; De Rivas, C.S. |
Filiación: | Depto. de Quimica Biológica, Fac. de Ciencias Exactas y Naturales, Ciudad Universitaria, Pabellón II (1428), Buenos Aires, Argentina
|
Palabras clave: | bacterial protein; amino acid sequence; article; bacillus sphaericus; bacillus thuringiensis; bacterial growth; environmental factor; nonhuman; osmoregulation; priority journal; protein determination; sporogenesis; stress; Bacillus; Bacillus thuringiensis; Bacterial Proteins; Osmotic Pressure; Peroxides; Sodium Chloride; Spores, Bacterial; Temperature; Ultraviolet Rays; Bacilli; Bacillus sphaericus; Bacillus subtilis; Bacillus thuringiensis; Bacillus thuringiensis serovar israelensis; Bacteria (microorganisms); Posibacteria |
Año: | 1998
|
Volumen: | 36
|
Número: | 4
|
Página de inicio: | 220
|
Página de fin: | 225
|
DOI: |
http://dx.doi.org/10.1007/s002849900298 |
Título revista: | Current Microbiology
|
Título revista abreviado: | Curr. Microbiol.
|
ISSN: | 03438651
|
CODEN: | CUMID
|
CAS: | Bacterial Proteins; Peroxides; Sodium Chloride, 7647-14-5
|
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03438651_v36_n4_p220_Cucchi |
Referencias:
- Baumann, L., Okmoto, K., Onterman, B.M., Lynch, M.J., Baumann, P., Phenotypic characterization of Bacillus thuringiensis and Bacillus cereus (1984) J Invertebr Pathol, 44, pp. 329-341
- Charles, J.F., Nielsen-LeRoux, C., Delécleuse, A., Bacillus sphaericus toxins: Molecular biology and mode of action (1996) Annu Rev Entomol, 41, pp. 451-472
- Csonka, L.N., Physiologial and genetic responses of bacteria to osmotic stress (1989) Microbiol Rev, 53, pp. 121-147
- Csonka, L.N., Hanson, A.D., Prokariotic osmoregulation: Genetics and physiology (1991) Annu Rev Microbiol, 45, pp. 569-606
- Cucchi, A., (1997) Supervivencia y Mejoramiento de Los Bacilos Entomopatógenos Bacillus Thuringiensis Var. Israelensis y Bacillus Sphaericus., pp. 1-127. , PhD Dissertation, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires
- Cucchi, A., Sanchez Rivas, C., Sensitivity of spores and growing cells of Bacillus thuringiensis var israelensis and Bacillus sphaericus to osmotic variations (1994) Curr Microbiol, 28, pp. 123-127
- Cucchi, A., Sanchez Rivas, C., Ssp genes and spore osmotolerance in Bacillus thuringiensis israelensis and Bacillus sphaericus (1995) Curr Microbiol, 31, pp. 228-233
- Davidson, E.W., Urbina, M., Payne, J., Mulla, J.S., Darwazeh, H., Dulmage, H., Correa, J.A., Fate of Bacillus sphaericus 1593 and 2362 spores used as larvicides in the aquatic environment (1984) Appl Env Microbiol, 47, pp. 125-129
- Griego, V.M., Spence, K.D., Inactivation of Bacillus thuringiensis spores by ultraviolet and visible light (1978) Appl Environ Microbiol, 35, pp. 906-910
- Johnson, W.C., Tipper, D.J., Acid-soluble spore proteins of Bacillus subtilis (1981) J Bacteriol, 146, pp. 972-982
- Lereclus, D., Delécluse, A., Lecadet, M.M., Diversity of Bacillus thuringiensis toxins and genes (1993) Bacillus Thuringiensis, and Environmental Biopesticide: Theory and Practice, pp. 37-69. , Entwistle PF, Cory JS, Bailey MJ, Higgs S (eds) New York: Wiley & Sons Ltd
- Loshon, C.A., Fliss, E.R., Setlow, B., Foerster, H.F., Setlow, P., Cloning and nucleotide sequencing of genes for Small, Acid-Soluble Spore Proteins of Bacillus cereus, Bacillus stearothermophilus and Thermoactinomyces thalpophilus (1986) J Bacteriol, 167, pp. 168-173
- Lowry, O.H., Rosenbrough, N.J., Farr, A.L., Randall, R.J., Protein measurement with the Folin phenol reagent (1951) J Biol Chem, 193, pp. 265-275
- Mason, J.M., Setlow, P., Essential role of small, acid-soluble spore proteins in resistance of Bacillus subtilis spores to UV light (1986) J Bacteriol, 167, pp. 174-178
- Mason, J.M., Hackett, R.H., Setlow, P., Regulation of expression of genes coding for small, acid-soluble proteins of Bacillus subtilis spores: Studies using lacZ gene fusions (1988) J Bacteriol, 170, pp. 239-244
- Nicholson, W.L., Setlow, P., Sporulation, gemination and outgrowth (1990) Molecular Biological Methods for Bacillus, pp. 391-450. , Hardwood CR, Cutting SM (eds) Chichester: John Wiley & Sons Ltd
- Nicolas, L., Bacteriological control of mosquitoes and blackflies: Present aspects and perspective research (1992) Pesqai Agropecu Bras, 27, pp. 37-46
- Nicolas, L., Dossou-Yovo, J., Hougard, J.M., Persistence and recycling of Bacillus sphaericus 2362 spores in Culex quinquefasciatus breeding sites in West Africa (1987) Appl Microbiol Biotechnol, 25, pp. 341-345
- Porter, A.G., Mosquitocidal toxins, genes and bacteria: The hit squad (1996) Parasitol Today, 5, pp. 175-179
- Porter, A.G., Davidson, E.W., Liu, J.W., Mosquitocidal toxins of bacilli and their genetic manipulation for effective biological control of mosquitoes (1993) Microbiol Rev, 57, pp. 838-861
- Priest, F.G., Biological control of mosquitoes and other biting flies by Bacillus sphaericus and Bacillus thuringiensis (1992) J Appl Bacteriol, 72, pp. 357-369
- Ruzal, S.M., Alice, A.F., Sanchez-Rivas, C., Osmoresistance of spores from Bacillus subtilis and the effects of ssp mutations (1994) Microbiology, 140, pp. 2173-2177
- Schägger, H., Von Jagow, G., Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa (1987) Anal Biochem, 166, pp. 368-379
- Setlow, P., Identification and localization of the major protein degraded during germination of Bacillus megaterium spores (1975) J Biol Chem, 250, pp. 8159-8167
- Setlow, P., Small acid-soluble, spore proteins of Bacillus species: Structure, synthesis, genetics, function and degradation (1988) Annu Rev Microbiol, 42, pp. 319-338
- Setlow, P., DNA in dormant spores of Bacillus species is in A-like conformation (1992) Mol Microbiol, 6, pp. 563-567
- Setlow, B., Setlow, P., Binding of small, acid-soluble spore proteins to DNA plays a significant role in the resistance of Bacillus subtilis spores to hydrogen peroxide (1993) Appl Environ Microbiol, 59, pp. 3418-3423
- Setlow, B., Hackett, R.H., Setlow, P., Noninvolvement of the spore cortex in acquisition of low-molecular weight basic proteins and UV light resistance during Bacillus sphaericus sporulation (1982) J Bacteriol, 149, pp. 494-498
- Setlow, B., Sun, D., Setlow, P., Interaction between DNA and α/β type small, acid-soluble spore proteins: A new class of DNA binding protein (1992) J Bacteriol, 174, pp. 2312-2322
- Vary, P.S., Tao, Y.P., Development of genetic methods in Bacillus megaterium (1988) Genetics and Biotechnology of Bacilli, 2, pp. 403-407. , Ganesan AT, Hoch JA (eds). New York: Academic Press
- Yasbin, R., Fields, P., Anderson, B., Properties of Bacillus subtilis 168 derivatives free of their natural prophages (1980) Gene, 12, pp. 155-159
- Yuan, K., Johnson, W.C., Tipper, D.J., Setlow, P., Comparison of various properties of low-molecular weight proteins from dormant spores of various Bacillus species (1981) J Bacteriol, 146, pp. 965-971
Citas:
---------- APA ----------
Cucchi, A. & De Rivas, C.S.
(1998)
. SASP (small, acid-soluble spore proteins) and spore properties in Bacillus thuringiensis israelensis and Bacillus sphaericus. Current Microbiology, 36(4), 220-225.
http://dx.doi.org/10.1007/s002849900298---------- CHICAGO ----------
Cucchi, A., De Rivas, C.S.
"SASP (small, acid-soluble spore proteins) and spore properties in Bacillus thuringiensis israelensis and Bacillus sphaericus"
. Current Microbiology 36, no. 4
(1998) : 220-225.
http://dx.doi.org/10.1007/s002849900298---------- MLA ----------
Cucchi, A., De Rivas, C.S.
"SASP (small, acid-soluble spore proteins) and spore properties in Bacillus thuringiensis israelensis and Bacillus sphaericus"
. Current Microbiology, vol. 36, no. 4, 1998, pp. 220-225.
http://dx.doi.org/10.1007/s002849900298---------- VANCOUVER ----------
Cucchi, A., De Rivas, C.S. SASP (small, acid-soluble spore proteins) and spore properties in Bacillus thuringiensis israelensis and Bacillus sphaericus. Curr. Microbiol. 1998;36(4):220-225.
http://dx.doi.org/10.1007/s002849900298