Artículo

La versión final de este artículo es de uso interno de la institución.
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

The importance of the DNA structure for the expression of the osmotic response (osmotolerance) was investigated in Bacillus subtilis 168. Plasmid pUB110 DNA was used as a reporter of the chromosomal DNA topology, and analyses were performed in chloroquine agarose gels. Plasmidic DNA obtained from cultures in Schaeffer medium (D) taken in those periods in which B. subtilis is able to express osmotolerance (early stationary phase or from germinating spores) or from adapted cultures to hyperosmotic medium (DN) presented a higher level of negative supercoiling than DNA samples from vegetative cultures, normally refractory to induction of osmotolerance. The involvement of the DNA gyrase was investigated through the sensitivity to novobiocin, an antibiotic inhibitor of its activity and the behavior of a gyrB1 mutant strain (RG1). In the wild-type strain, the addition of a sublethal concentration of novobiocin (0.5 μg/ml) to the hyperosmotic medium relaxed DNA and inhibited growth. Moreover, already growing cultures in DN medium and later submitted to the same antibiotic presented a relaxed DNA and stopped growing. The RG1 mutant strain submitted to similar novobiocin treatments displayed normal growth in DN novobiocin medium. These results pointed to the requirement of a highly negative supercoiled DNA structure involving the gyrase activity in osmotic response.

Registro:

Documento: Artículo
Título:DNA supercoiling and osmoresistance in Bacillus subtilis 168
Autor:Alice, A.F.; Sanchez-Rivas, C.
Filiación:Departmento de Quim. Biol., Universidad de Buenos Aires, Pabellón II, (1428) Buenos Aires, Argentina
Palabras clave:dna topoisomerase (atp hydrolysing); novobiocin; plasmid dna; article; bacillus subtilis; bacterial growth; bacterium mutant; dna supercoiling; enzyme activity; nonhuman; osmoregulation; priority journal; Anti-Bacterial Agents; Bacillus subtilis; Chloroquine; Culture Media; DNA Gyrase; DNA Topoisomerases, Type II; DNA, Bacterial; DNA, Superhelical; Genes, Reporter; Novobiocin; Osmotic Pressure; Plasmids; Spores, Bacterial; Bacillus subtilis; Bacteria (microorganisms); bacterium subtilis
Año:1997
Volumen:35
Número:5
Página de inicio:309
Página de fin:315
DOI: http://dx.doi.org/10.1007/s002849900260
Título revista:Current Microbiology
Título revista abreviado:CURR. MICROBIOL.
ISSN:03438651
CODEN:CUMID
CAS:Anti-Bacterial Agents; Chloroquine, 54-05-7; Culture Media; DNA Gyrase, EC 5.99.1.-; DNA Topoisomerases, Type II, EC 5.99.1.3; DNA, Bacterial; DNA, Superhelical; Novobiocin, 303-81-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03438651_v35_n5_p309_Alice

Referencias:

  • Arnold-Schulz-Gahmen, B., Salti-Montesanto, V., Nguyen, J., Hirschbein, L., Le Hégarat, F., The Bacillus subtilis nucleoid-associated protein PB12 strongly compacts DNA (1994) J Bacteriol, 176, pp. 50-60
  • Birnboim, H., Doly, J., A rapid alkaline extraction procedure for screening recombinant plasmid DNA (1979) Nucleic Acid Res, 7, pp. 897-903
  • Boch, J., Kempf, B., Bremer, E., Osmoregulation in Bacillus subtilis: Synthesis of the osmoprotectant glycine betaine from exogenously provided choline (1994) J Bacteriol, 176, pp. 5364-5371
  • Bron, S., Plasmids (1990) Molecular Biological Methods for Bacillus, pp. 75-136. , Harwood CR, Cutting SM (eds.). Chichester, UK: J Wiley and Sons Ltd
  • Chang, S., Cohen, S.N., High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA (1979) Mol Gen Genet, 168, pp. 111-115
  • Cozzarelli, N., DNA gyrase and the supercoiling of DNA (1981) Science, 207, pp. 953-960
  • Drlica, K., Biology of bacterial deoxyribonucleic acid topoisomerases (1984) Microbiol Rev, 48, pp. 273-289
  • Drlica, K., Control of bacterial DNA supercoiling (1992) Mol Microbiol, 6, pp. 425-433
  • Drlica, K., Rouviere-Yaniv, J., Histone-like proteins of bacteria (1987) Microbiol Rev, 51, pp. 301-319
  • Gellert, M., O'Dea, M.H., Itoh, T., Tomizawa, J.I., Novobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase (1976) Proc Natl Acad Sci USA, 73, pp. 4474-4478
  • Goldstein, E., Drlica, K., Regulation of bacterial DNA supercoiling: Plasmid linking numbers vary with growth temperature (1984) Proc Natl Acad Sci USA, 81, pp. 4046-4050
  • Graeme-Cook, K.A., May, G., Bremer, E., Higgins, C.F., Osmotic regulation of porin expression: A role for DNA supercoiling (1989) Mol Microbiol, 3, pp. 1287-1294
  • Grau, R., Gardiol, D., Glikin, G.C., De Mendoza, D., DNA supercoiling and thermal regulation of unsaturated fatty acid synthesis in Bacillus subtilis (1994) Mol Microbiol, 11, pp. 933-941
  • Griffith, J., Makhov, A., Santiago-Lara, L., Setlow, P., Electron microscopic studies of the interaction between a Bacillus subtilis α/β-type small, acid-soluble spore protein with DNA: Protein binding is cooperative, stiffens the DNA, and induces negative supercoiling (1994) Proc Natl Acad Sci USA, 91, pp. 8224-8228
  • Higgins, C.F., Dorman, C.J., Stirling, D.A., Waddell, L., Booth, I.R., May, G., Bremer, E., A physiological role for DNA supercoiling in the osmotic regulation of gene expression in S. typhimurium and E. coli (1988) Cell, 52, pp. 569-584
  • Hsieh, L.S., Rouviere-Yaniv, J., Drlica, K., Bacterial DNA supercoiling and [ATP]/[ADP] ratio: Changes associated with salt shock (1991) J Bacteriol, 173, pp. 3914-3917
  • Jordi, B.J., Owen-Hughes, A.M., Hulton, C.S.J., Higgins, C.F., DNA twist, flexibility and transcription of the osmoregulated proU promoter of Salmonella typhimurium (1995) EMBO J, 14, pp. 5690-5700
  • Kempf, B., Bremer, E., OpuA, an osmotically regulated binding protein-dependent transport system for the osmoprotectant glycine betaine in Bacillus subtilis (1995) J Biol Chem, 270, pp. 16701-16713
  • Krispin, O., Allmansberger, R., Changes in DNA supertwist as a response of Bacillus subtilis towards different kinds of stress (1995) FEMS Microbiol Lett, 134, pp. 129-135
  • Kunst, F., Rapoport, G., Salt stress is an environmental signal affecting degradative enzyme synthesis in Bacillus subtilis (1995) J Bacteriol, 177, pp. 2403-2407
  • Le Hégarat, F., Guillen, N., Fleury, A.M., Hirschbein, L., Comparative studies on purified nucleoids of Bacillus subtilis during growth and sporulation (1978) Spore VII, pp. 193-199. , Halvorson and Campel (eds). Washington: AMS Publications
  • Liu, L.F., Wang, J.C., Supercoiling of the DNA template during transcription (1987) Proc Natl Acad Sci USA, 84, pp. 7024-7027
  • Lucht, J.M., Dersch, P., Kempf, B., Bremer, E., Interactions of the nucleoid-associated DNA-binding protein H-NS with the regulatory region of the osmotically controlled proU operon of Escherichia coli (1994) J Biol Chem, 269, pp. 6578-6586
  • McClellan, J.A., Boublíková, P., Palecek, Lilley, D.M., Superhelical torsion in cellular DNA responds directly to environmental and genetic factors (1990) Proc Natl Acad Sci USA, 87, pp. 8373-8377
  • Micka, B., Marahiel, M., The DNA-binding protein Hbsu is essential for the normal growth and development in Bacillus subtilis (1992) Biochimie, 74, pp. 641-650
  • Micka, B., Groch, N., Heinemann, U., Marahiel, M., Molecular cloning, nucleotide sequence, and characterization of the Bacillus subtilis gene encoding the DNA-binding protein Hbsu (1991) J Bacteriol, 173, pp. 3191-3198
  • Ni Bhriain, N., Dorman, C.J., Higgins, C.F., An overlap between osmotic and anaerobic stress response: A potential role for DNA supercoiling in the coordinate regulation of gene expression (1989) Mol Microbiol, 3, pp. 933-942
  • Nicholson, W.L., Setlow, P., Dramatic increase in negative superhelicity of plasmid DNA in the forespore compartment of sporulating cells of Bacillus subtilis (1990) J Bacteriol, 172, pp. 7-14
  • Owen-Hughes, T.A., Pavitt, G.D., Santos, D.S., Sidebotham, J.M., Hulton, C.S.J., Hinton, J.C.D., Higgins, C.F., The chromatin-associated protein H-NS interacts with curved DNA to influence DNA topology and gene expression (1992) Cell, 71, pp. 255-265
  • Pruss, G.J., Drlica, K., DNA supercoiling and prokaryotic transcription (1989) Cell, 56, pp. 521-523
  • Rubinstein, C.P., Moratinos, L.C., Coso, O.A., Sanchez-Rivas, C., Improvements in the transformation of Bacillus subtilis protoplasts with plasmid DNA (1988) FEMS Microbiol Lett, 56, pp. 67-70
  • Ruzal, S.M., Sanchez-Rivas, C., Physiological and genetic characterization of the osmotic stress response in Bacillus subtilis (1994) Can J Microbiol, 40, pp. 140-144
  • Ruzal, S.M., Alice, A.F., Sanchez-Rivas, C., Osmotolerance of spores from Bacillus subtilis and the effect of ssp mutations (1994) Microbiology, 140, pp. 2173-2177
  • Salti, V., Le Hégarat, F., Hirschbein, L., Isolation and characterization of small heat-stable acid soluble DNA-binding proteins from Bacillus subtilis (1985) J Gen Microbiol, 131, pp. 581-590
  • Schaeffer, P., Millet, J., Aubert, J., Catabolic repression of bacterial sporulation (1965) Proc Natl Acad Sci USA, 54, pp. 701-711
  • Sheehan, B.J., Foster, T.J., Dorman, C.J., Park, S., Stewart, G.S.A.B., Osmotic and growth-phase dependent regulation of the eta gene of Staphylococcus aureus: A role for DNA supercoiling (1992) Mol Gen Genet, 232, pp. 49-57
  • Tanaka, H., Yasuzawa, K., Kohno, K., Goshima, N., Kano, Y., Saiki, T., Imamoto, F., Role of HU proteins in forming and constraining supercoils of chromosomal DNA in Escherichia coli (1995) Mol Gen Genet, 248, pp. 518-526
  • Wang, J.Y., Syvanen, M., DNA twist as a transcriptional sensor for environmental changes (1992) Mol Microbiol, 6, pp. 1861-1866
  • Whatmore, A.M., Chudek, J.A., Reed, R.H., The effects of osmotic upshock on the intracellular solute pools of Bacillus subtilis (1990) J Gen Microbiol, 136, pp. 2527-2535

Citas:

---------- APA ----------
Alice, A.F. & Sanchez-Rivas, C. (1997) . DNA supercoiling and osmoresistance in Bacillus subtilis 168. Current Microbiology, 35(5), 309-315.
http://dx.doi.org/10.1007/s002849900260
---------- CHICAGO ----------
Alice, A.F., Sanchez-Rivas, C. "DNA supercoiling and osmoresistance in Bacillus subtilis 168" . Current Microbiology 35, no. 5 (1997) : 309-315.
http://dx.doi.org/10.1007/s002849900260
---------- MLA ----------
Alice, A.F., Sanchez-Rivas, C. "DNA supercoiling and osmoresistance in Bacillus subtilis 168" . Current Microbiology, vol. 35, no. 5, 1997, pp. 309-315.
http://dx.doi.org/10.1007/s002849900260
---------- VANCOUVER ----------
Alice, A.F., Sanchez-Rivas, C. DNA supercoiling and osmoresistance in Bacillus subtilis 168. CURR. MICROBIOL. 1997;35(5):309-315.
http://dx.doi.org/10.1007/s002849900260