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Abstract:

The effect of different nonionic surfactants (Tween 80, Tween 20, Triton X-100) and polyethylene glycol (PEG 6000) was tested on cellulolytic enzyme system production. Tween 80 gave the highest yield of endoglucanase, exoglucanase, and cellobiase at the 20th day of growth, presumably by causing increased permeability of cell membranes and/or by promoting the release of cell-bound enzymes. Maximal yield of endoglucanase was achieved with 1.7 mM Tween 80, whereas exoglucanase and cellobiase were at 0.85 mM. In the same way, this compound increased fungal growth. On the other hand, Tween 20 and Triton X-100 inhibited growth and cellulolytic enzyme production. High yields of endoglucanase and exoglucanase were achieved with PEG 6000 in comparison with the control, presumably by increasing enzyme stability.

Registro:

Documento: Artículo
Título:Effect of surfactants on cellulase production by Nectria catalinensis
Autor:Pardo, A.G.
Filiación:Lab. de Micología Exp., Depto. de Ciencias Biológicas, Universidad de Buenos Aires, 1428-Buenos Aires, Argentina
Palabras clave:beta glucosidase; cellulase; glucan synthase; surfactant; article; cell membrane permeability; enzyme activity; enzyme release; enzyme stability; enzyme synthesis; fungus growth; nonhuman; priority journal
Año:1996
Volumen:33
Número:4
Página de inicio:275
Página de fin:278
DOI: http://dx.doi.org/10.1007/s002849900113
Título revista:Current Microbiology
Título revista abreviado:CURR. MICROBIOL.
ISSN:03438651
CODEN:CUMID
CAS:beta glucosidase, 51683-43-3, 9001-22-3; cellulase, 9012-54-8; glucan synthase, 55126-98-2, 74191-29-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03438651_v33_n4_p275_Pardo

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Citas:

---------- APA ----------
(1996) . Effect of surfactants on cellulase production by Nectria catalinensis. Current Microbiology, 33(4), 275-278.
http://dx.doi.org/10.1007/s002849900113
---------- CHICAGO ----------
Pardo, A.G. "Effect of surfactants on cellulase production by Nectria catalinensis" . Current Microbiology 33, no. 4 (1996) : 275-278.
http://dx.doi.org/10.1007/s002849900113
---------- MLA ----------
Pardo, A.G. "Effect of surfactants on cellulase production by Nectria catalinensis" . Current Microbiology, vol. 33, no. 4, 1996, pp. 275-278.
http://dx.doi.org/10.1007/s002849900113
---------- VANCOUVER ----------
Pardo, A.G. Effect of surfactants on cellulase production by Nectria catalinensis. CURR. MICROBIOL. 1996;33(4):275-278.
http://dx.doi.org/10.1007/s002849900113