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Abstract:

It was shown previously that spores and vegetative cells of Bacillus sphaericus (Bf) and Bacillus thuringiensis israelensis (Bti) are very sensitive to osmotic variations. Since spore osmotolerance has been associated with their SASP (small acid soluble spore proteins) content coded by ssp genes, hybridization assays were performed with sspE and sspA genes from B. subtilis as probes and showed that Bti and Bf strains could lack an sspE-like gene. The B. subtilis sspE gene was then introduced into Bti 4Q2 strain; spores were obtained and showed a 65 to 650 times higher level of osmotolerance to NaCl, without affecting other important properties: hypoosmotic resistance in vegetative cells, spore UV resistance, and larvicidal activity against diptera larvae. © 1995 Springer-Verlag New York Inc.

Registro:

Documento: Artículo
Título:ssp Genes and spore osmotolerance in Bacillus thuringiensis israelensis and Bacillus sphaericus
Autor:Cucchi, A.; De Rivas, C.S.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria Pabellón II 4cPiso (1428), Buenos Aires, Argentina
Palabras clave:article; bacillus sphaericus; bacillus thuringiensis; bacterial gene; bacterial spore; hybridization; nonhuman; osmotic stress; priority journal; sporogenesis; ultraviolet radiation; Animal; Bacillus thuringiensis; Bacterial Proteins; Diptera; Genes, Bacterial; Larva; Pest Control, Biological; Species Specificity; Spores, Bacterial; Support, Non-U.S. Gov't; Transfection; Ultraviolet Rays; Water-Electrolyte Balance; Bacillus sphaericus; Bacillus subtilis; Bacillus thuringiensis serovar israelensis; Bacteria (microorganisms); Diptera
Año:1995
Volumen:31
Número:4
Página de inicio:228
Página de fin:233
DOI: http://dx.doi.org/10.1007/BF00298379
Título revista:Current Microbiology
Título revista abreviado:Curr. Microbiol.
ISSN:03438651
CODEN:CUMID
CAS:Bacillus spore-specific proteins; Bacterial Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03438651_v31_n4_p228_Cucchi

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Citas:

---------- APA ----------
Cucchi, A. & De Rivas, C.S. (1995) . ssp Genes and spore osmotolerance in Bacillus thuringiensis israelensis and Bacillus sphaericus. Current Microbiology, 31(4), 228-233.
http://dx.doi.org/10.1007/BF00298379
---------- CHICAGO ----------
Cucchi, A., De Rivas, C.S. "ssp Genes and spore osmotolerance in Bacillus thuringiensis israelensis and Bacillus sphaericus" . Current Microbiology 31, no. 4 (1995) : 228-233.
http://dx.doi.org/10.1007/BF00298379
---------- MLA ----------
Cucchi, A., De Rivas, C.S. "ssp Genes and spore osmotolerance in Bacillus thuringiensis israelensis and Bacillus sphaericus" . Current Microbiology, vol. 31, no. 4, 1995, pp. 228-233.
http://dx.doi.org/10.1007/BF00298379
---------- VANCOUVER ----------
Cucchi, A., De Rivas, C.S. ssp Genes and spore osmotolerance in Bacillus thuringiensis israelensis and Bacillus sphaericus. Curr. Microbiol. 1995;31(4):228-233.
http://dx.doi.org/10.1007/BF00298379