Artículo

Sersa, G.; Teissie, J.; Cemazar, M.; Signori, E.; Kamensek, U.; Marshall, G.; Miklavcic, D. "Electrochemotherapy of tumors as in situ vaccination boosted by immunogene electrotransfer" (2015) Cancer Immunology, Immunotherapy. 64(10):1315-1327
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Abstract:

Electroporation is a platform technology for drug and gene delivery. When applied to cell in vitro or tissues in vivo, it leads to an increase in membrane permeability for molecules which otherwise cannot enter the cell (e.g., siRNA, plasmid DNA, and some chemotherapeutic drugs). The therapeutic effectiveness of delivered chemotherapeutics or nucleic acids depends greatly on their successful and efficient delivery to the target tissue. Therefore, the understanding of different principles of drug and gene delivery is necessary and needs to be taken into account according to the specificity of their delivery to tumors and/or normal tissues. Based on the current knowledge, electrochemotherapy (a combination of drug and electric pulses) is used for tumor treatment and has shown great potential. Its local effectiveness is up to 80 % of local tumor control, however, without noticeable effect on metastases. In an attempt to increase systemic antitumor effectiveness of electrochemotherapy, electrotransfer of genes with immunomodulatory effect (immunogene electrotransfer) could be used as adjuvant treatment. Since electrochemotherapy can induce immunogenic cell death, adjuvant immunogene electrotransfer to peritumoral tissue could lead to locoregional effect as well as the abscopal effect on distant untreated metastases. Therefore, we propose a combination of electrochemotherapy with peritumoral IL-12 electrotransfer, as a proof of principle, using electrochemotherapy boosted with immunogene electrotransfer as in situ vaccination for successful tumor treatment. © 2015, The Author(s).

Registro:

Documento: Artículo
Título:Electrochemotherapy of tumors as in situ vaccination boosted by immunogene electrotransfer
Autor:Sersa, G.; Teissie, J.; Cemazar, M.; Signori, E.; Kamensek, U.; Marshall, G.; Miklavcic, D.
Filiación:Department of Experimental Oncology, Institute of Oncology Ljubljana, Zaloska 2, Ljubljana, 1000, Slovenia
IPBS (Institut de Pharmacologie et de Biologie Structurale), CNRS, 205 route de Narbonne BP64182, Toulouse, 31077, France
UPS, IPBS, Université de Toulouse, Toulouse, 31077, France
University of Primorska, Polje 42, Izola, 6310, Slovenia
Laboratory of Molecular Pathology and Experimental Oncology, CNR-Institute of Translational Pharmacology, Via Fosso del Cavaliere 100, Rome, 00133, Italy
Laboratorio de Sistemas Complejos, Departamento de Computación e Instituto de Física del Plasma, CONICET, Universidad de Buenos Aires, Buenos Aires, C1428EGA, Argentina
University of Ljubljana, Trzaska 25, Ljubljana, 1000, Slovenia
Palabras clave:Abscopal effect; Electrochemotherapy; Electroporation; Gene electrotransfer; PIVAC 14; Vaccination; interleukin 12; plasmid DNA; small interfering RNA; interleukin 12; adjuvant therapy; cancer control; cancer immunization; cell death; cell density; cell junction; cell size; cell surface; cell volume; cytotoxic T lymphocyte; electric conductivity; electric field; electrochemotherapy; electrophoresis; electroporation; gene targeting; human; immune response; immune status; immunogenicity; immunoreactivity; in vitro study; in vivo study; inflammatory cell; membrane permeability; memory T lymphocyte; nonhuman; priority journal; Review; target organ; Th1 cell; animal; drug delivery system; gene transfer; Neoplasms; procedures; vaccination; Animals; Drug Delivery Systems; Electrochemotherapy; Gene Transfer Techniques; Humans; Interleukin-12; Neoplasms; Vaccination
Año:2015
Volumen:64
Número:10
Página de inicio:1315
Página de fin:1327
DOI: http://dx.doi.org/10.1007/s00262-015-1724-2
Título revista:Cancer Immunology, Immunotherapy
Título revista abreviado:Cancer Immunol. Immunother.
ISSN:03407004
CODEN:CIIMD
CAS:interleukin 12, 138415-13-1; Interleukin-12
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03407004_v64_n10_p1315_Sersa

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Citas:

---------- APA ----------
Sersa, G., Teissie, J., Cemazar, M., Signori, E., Kamensek, U., Marshall, G. & Miklavcic, D. (2015) . Electrochemotherapy of tumors as in situ vaccination boosted by immunogene electrotransfer. Cancer Immunology, Immunotherapy, 64(10), 1315-1327.
http://dx.doi.org/10.1007/s00262-015-1724-2
---------- CHICAGO ----------
Sersa, G., Teissie, J., Cemazar, M., Signori, E., Kamensek, U., Marshall, G., et al. "Electrochemotherapy of tumors as in situ vaccination boosted by immunogene electrotransfer" . Cancer Immunology, Immunotherapy 64, no. 10 (2015) : 1315-1327.
http://dx.doi.org/10.1007/s00262-015-1724-2
---------- MLA ----------
Sersa, G., Teissie, J., Cemazar, M., Signori, E., Kamensek, U., Marshall, G., et al. "Electrochemotherapy of tumors as in situ vaccination boosted by immunogene electrotransfer" . Cancer Immunology, Immunotherapy, vol. 64, no. 10, 2015, pp. 1315-1327.
http://dx.doi.org/10.1007/s00262-015-1724-2
---------- VANCOUVER ----------
Sersa, G., Teissie, J., Cemazar, M., Signori, E., Kamensek, U., Marshall, G., et al. Electrochemotherapy of tumors as in situ vaccination boosted by immunogene electrotransfer. Cancer Immunol. Immunother. 2015;64(10):1315-1327.
http://dx.doi.org/10.1007/s00262-015-1724-2