Abstract:
FC-2.15 is a murine IgM monoclonal antibody (mAb) that recognizes a cell-surface antigen (Ag2.15) expressed in most tumor-proliferating cells of human breast carcinomas and other neoplasias. In this study the cytotoxic ability of mAb FC-2.15, its cell-surface binding properties and endocytosis in Ag2.15-expressing (Ag2.15+) cells were investigated. A51Cr-release assay was used to test the FC-2.15-mediated cytotoxicity. When human serum was used as source of complement, FC-2.15 exerted a strong cytotoxic effect against human Ag2.15+ cells such as MCF-7 (breast cancer cell line), primary breast carcinoma cells, polymorphonuclear leukocytes and chronic myeloid leukemia cells. The mAb concentration range was 1-50 μg/ml. Cytotoxicity was completely abolished when complement was inactivated. Only 3.8±2.9% of MCF-7 cells survived the treatment with FC-2.15 in the presence of human serum. A flow-cytometry assay was performed to study the Ag2.15 expression of the surviving cells and they were found to be Ag2.15-. FC-2.15 did not mediate antibody-dependent cell cytotoxicity when different effector cells were used. Scatchard analysis with125I-FC-2.15 on MCF-7 cells demonstrated an affinity constant of 6.9×107 M-1 and 2.8×106 antigenic sites/cell.125I-FC-2.15 was internalized to cytoplasmic vesicles reaching a maximum of 27% after 6 h incubation, followed by the release of labeled degradation products to the supernatant. FC-2.15 appears to exert its cytotoxic effect mainly in the presence of human complement, it reacts with intermediate affinity with a high-density surface antigen, and it is slowly internalized by Ag2.15+ cells. © 1995 Springer-Verlag.
Registro:
Documento: |
Artículo
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Título: | Functional properties of FC-2.15, a monoclonal antibody that mediates human complement cytotoxicity against breast cancer cells |
Autor: | Ballaré, C.; Barrio, M.; Portela, P.; Mordoh, J. |
Filiación: | Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina Instituto de Investigaciones Bioquímicas 'Fundación Campomar', Patricias Argentinas 435, 1405, Argentina
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Palabras clave: | Breast cancer; Complement; Cytotoxicity; Monoclonal antibody (mAb); monoclonal antibody; monoclonal antibody fc 2 15; unclassified drug; article; breast cancer; cancer cell culture; controlled study; cytotoxicity; female; human; human cell; priority journal; Antibodies, Monoclonal; Antibody-Dependent Cell Cytotoxicity; Antigen-Antibody Complex; Antigens, Neoplasm; Breast Neoplasms; Complement; Complement Activation; Cytotoxicity, Immunologic; Endocytosis; Human; In Vitro; Support, Non-U.S. Gov't; Tumor Cells, Cultured |
Año: | 1995
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Volumen: | 41
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Número: | 1
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Página de inicio: | 15
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Página de fin: | 22
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DOI: |
http://dx.doi.org/10.1007/BF01788955 |
Título revista: | Cancer Immunology Immunotherapy
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Título revista abreviado: | Cancer Immunol Immunother
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ISSN: | 03407004
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CODEN: | CIIMD
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CAS: | Antibodies, Monoclonal; Antigen-Antibody Complex; Antigens, Neoplasm; Complement, 9007-36-7
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03407004_v41_n1_p15_Ballare |
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Citas:
---------- APA ----------
Ballaré, C., Barrio, M., Portela, P. & Mordoh, J.
(1995)
. Functional properties of FC-2.15, a monoclonal antibody that mediates human complement cytotoxicity against breast cancer cells. Cancer Immunology Immunotherapy, 41(1), 15-22.
http://dx.doi.org/10.1007/BF01788955---------- CHICAGO ----------
Ballaré, C., Barrio, M., Portela, P., Mordoh, J.
"Functional properties of FC-2.15, a monoclonal antibody that mediates human complement cytotoxicity against breast cancer cells"
. Cancer Immunology Immunotherapy 41, no. 1
(1995) : 15-22.
http://dx.doi.org/10.1007/BF01788955---------- MLA ----------
Ballaré, C., Barrio, M., Portela, P., Mordoh, J.
"Functional properties of FC-2.15, a monoclonal antibody that mediates human complement cytotoxicity against breast cancer cells"
. Cancer Immunology Immunotherapy, vol. 41, no. 1, 1995, pp. 15-22.
http://dx.doi.org/10.1007/BF01788955---------- VANCOUVER ----------
Ballaré, C., Barrio, M., Portela, P., Mordoh, J. Functional properties of FC-2.15, a monoclonal antibody that mediates human complement cytotoxicity against breast cancer cells. Cancer Immunol Immunother. 1995;41(1):15-22.
http://dx.doi.org/10.1007/BF01788955