Artículo

Duboscq, C.; Quintana, I.; Bassilotta, E.; Bergonzelli, G.E.; Porterie, P.; Sassetti, B.; Haedo, A.S.; Wainsztein, N.; Kruithof, E.K.O.; Kordich, L. "Plasminogen: An important hemostatic parameter in septic patients" (1997) Thrombosis and Haemostasis. 77(6):1090-1095
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Abstract:

Background: Previously we observed in some but not all septic patients a low plasma concentration of plasminogen. Objectives: To investigate prospectively whether plasma levels of plasminogen or the ratio of plasminogen to alpha-2-antiplasmin have a prognostic value for survival from sepsis and to study the variation of other hemostatic parameters during septicemia. Patients: The study population consisted of 45 consecutive patients with septicemia, 15 non-septic patients from the same intensive care unit and 30 healthy volunteers. Measurements and Main Results: Plasminogen concentrations were significantly lower (p < 0.001) in plasma of septic patients (median 0,62 IU/ml range: 0.15-1,06) than in plasma of healthy controls (median 1.00 IU/ml, range: 0.75-1.10) or of non-septic intensive care patients (median 1.00 IU/ml, range: 0.82-1.08). Among the other parameters tested, plasminogen activator inhibitor (PAI-1) antigen concentration and PAI activity were similar in septic and non-septic intensive care patients, but higher than in healthy controls. Concentrations of elastase-alpha-1-protease inhibitor or of thrombin-antithrombin complexes were higher in septic patients than in non-septic intensive care patients or healthy controls. A degraded form of plasminogen of 38 kDa was detected by Western blot analysis in the plasma of septic patients, but not in plasma of non-septic intensive care patients or controls. Plasminogen alone or the ratio of plasminogen to antiplasmin were good markers for survival from septicemia. E.g. for plasminogen at a cut off of 0.65 IU/ml, sensitivity was 90.5% and specificity 66.7%, whereas for the ratio of plasminogen over antiplasmin at a cut off ratio of 0,65 IU/ml, sensitivity was 95.2% and specificity 70.8%. Conclusion: Plasminogen or the ratio of plasminogen to antiplasmin are sensitive markers for survival in patients with septicemia.

Registro:

Documento: Artículo
Título:Plasminogen: An important hemostatic parameter in septic patients
Autor:Duboscq, C.; Quintana, I.; Bassilotta, E.; Bergonzelli, G.E.; Porterie, P.; Sassetti, B.; Haedo, A.S.; Wainsztein, N.; Kruithof, E.K.O.; Kordich, L.
Filiación:Dpto. de Quim. Biológica, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Gastroenterology Division, University Hospital, Lausanne, Switzerland
Department of Critical Care, Hospital Privado Güemes, Buenos Aires, Argentina
Division of Angiology and Hemostasis, University Hospital, Geneva, Switzerland
Palabras clave:alpha 2 antiplasmin; antiplasmin; antithrombin; elastase alpha 1 protease inhibitor; elastase inhibitor; plasminogen; plasminogen activator inhibitor; thrombin; unclassified drug; adult; aged; article; blood clotting; controlled study; enzyme activity; fibrinolysis; hemostasis; human; immunoblotting; intensive care; major clinical study; priority journal; prognosis; protein blood level; protein degradation; sepsis; septicemia; Adult; Aged; Antiplasmin; Hemostasis; Humans; Middle Aged; Plasminogen; Prognosis; Prospective Studies; Sepsis
Año:1997
Volumen:77
Número:6
Página de inicio:1090
Página de fin:1095
Título revista:Thrombosis and Haemostasis
Título revista abreviado:THROMB. HAEMOST.
ISSN:03406245
CODEN:THHAD
CAS:Antiplasmin; Plasminogen, 9001-91-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03406245_v77_n6_p1090_Duboscq

Referencias:

  • Bone, R.C., Balk, R.A., Cerra, F.B., Dellinger, R.P., Fein, A.M., Knaus, W.A., Schein, R.M., Sibbad, W., Definitions for sepsis and organ failure and guidelines for the use of innovative therapies in sepsis (1992) Chest, 10, pp. 1644-1655. , The American College of Chest Physicians/Society of Critical Care Medicine Consensus Conference Committee
  • Bone, R.C., The pathogenesis of sepsis (1991) Ann Intern Med, 115, pp. 457-469
  • Coalson, J.J., Pathology of sepsis, septic shock and multiple organ failure (1986) Perspective on Sepsis and Septic Shock, pp. 27-57. , Sibbald WJ, Sprung CL (eds). Society of Critical Care Medicine. Fullefon CA
  • Philippé, J., Offner, F., Declerck, P.J., Leroux-Roels, G., Vogelaers, D., Basele, G., Collen, D., Fibrinolysis and coagulation in patients with infectious disease and sepsis (1991) Thromb Haemost, 65, pp. 291-295
  • Hesselvik, F.J., Blombäck, M., Brodin, B., Mailer, R., Coagulation, Fibrinolysis and Kallikrein systems in sepsis: Relation to outcome (1989) Crit Care Med, 17, pp. 724-733
  • Dofferhoff, A.S., Bom, V.J., De Vries-Hospers, H.G., Van Ingen, J., Van De Meer, J., Hazenberg, B.P., Mulder, P.O., Weits, J., Pattern of cytokines, plasma endotoxin, plasminogen activator inhibition and acute-phase proteins during the treatment of severe sepsis in human (1992) Crit Care Med, 20, pp. 185-192
  • Salgado, A., Boveda, J.L., Monasterio, J., Segura, R.M., Mourelle, M., Gomez-Jimenez, J., Peracaula, R., Inflammatory mediators and their influence on haemostasis (1994) Haemost, 24, pp. 132-138
  • Silverman, P., Goldsmith, G.H., Spitzer, T.R., Rehmus, E.H., Berger, N.A., Effect of tumor necrosis factor on the human fibrinolytic system (1990) J Clinical Oncology, 8, pp. 468-475
  • Hellgren, M., Egberg, N., Klund, E.J., Blood Coagulation and fibrinolytic factors and their inhibitors in critically ill patients (1984) Intens Care Med, 10, pp. 23-28
  • Seitz, R., Wolf, M., Egbring, R., Radtke, K.P., Leisenfeld, A., Pittner, P., Havemam, K., Participation and interactions of neutrophil elastase in haemostatic disorders of patients with severe infections (1987) Eur J Haematology, 38, pp. 231-240
  • Plow, E.F., The major fibrinolytic proteases of human leukocytes (1980) Biochim Biophys Acta, 630, pp. 47-56
  • Moroz, L., Miniplasminogen: A mechanism for leukocyte modulation of plasminogen activation by urokinase (1981) Blood, 103, pp. 58-97
  • Machovich, R., Owen, W., The elastase mediated pathway of fibrinolysis (1990) Blood Coagulation and Fibrinolysis, 1, pp. 79-90
  • Christensen, U., Sottrup-Jensen, L., Magnusson, S., Petersen, T., Clemmensen, I., Enzymic properties of the neoplasmin -val 442 (miniplasmin) (1979) Biochim Biophy Acta, 567, pp. 472-481
  • Kordich, L.C., Porterie, V.P., Lago, O., Bergonzelli, G.E., Sassetti, B., Sanchez Avalos, J.C., Mini-plasminogen like molecule in septic patients (1987) Thromb Res, 47, pp. 553-560
  • Molinas, F.C., Kordich, L.C., Porterie, P., Lerner, G., Maiztegui, J.I., Plasminogen abnormalities in patients with Argentine hemorrhagic fever (1987) Thromb Res, 48, pp. 713-720
  • Deutsch, D.G., Mertz, E.T., Plasminogen: Purification from human plasma by affinity chromatography (1970) Science, 170, pp. 1095-1096
  • Laemmli, U.K., Cleavage of structural proteins during assembly of the head of bacteriophage T4 (1970) Nature, 227, pp. 680-685
  • Towbin, H., Stochelim, T., Gordon, J., Electrophoretic transfer of proteins from polyacrilamide gels to nitrocelulose sheets: Procedure and some applications (1979) Proc Natl Acad Sci USA, 76, pp. 4350-4354
  • Zweig, M.H., Campbell, G., Receiver-Operating Characteristic (ROC) plots: A fundamental evaluation tool in clinical medicine (1993) Clin Chem, 39, pp. 561-577
  • Pralong, G., Calandra, T., Glauser, M.P., Schellekens, J., Verhoef, J., Bachmann, F., Kruithof, E.K.O., Plasminogen activator inhibitor 1: A new prognostic marker in septic shock (1989) Thromb Haemost, 61, pp. 459-462
  • Maestries, J.C., Kruithof, E.K.O., Marie-Paule Gascon, M.P., Herodin, F., Agay, D., Ythier, A., In vivo modulation of coagulation and fibrinolysis by recombinant glycosylated human interleukin 6 in baboons (1994) Eur Cytokine Netw, 5, pp. 275-281
  • Kluft, C., Verheyen, J.H., Jie, A.F.H., Rijken, D.C., Preston, F.E., Sue Ling, H.M., Jespersen, J., Aasen, A.O., The postoperative fibrinolytic shutdown: A rapidly reverting acute phase patterns for the fast-acting inhibitor of tissue-type plasminogen activator after trauma (1985) Scand J Clin Lab Invest, 45, pp. 605-610
  • Lorente, J.A., Garcia-Frade, L.J., Landin, M., De Pablo, R., Torrado, C., Renes, E., Garcia Avello, A., Time course of hemostatic abnormalities in sepsis and its relation to outcome (1993) Chest, 103, pp. 1536-1542
  • Kruithof, E.K.O., Calandra, T., Pralong, G., Heumann, D., Gérain, J., Baumgartner, J.D., Bachmann, F., Glause, M.P., Evolution of plasminogen activator inhibitor type 1 in patients with septic shock - Correlation with cytokine concentrations (1993) Fibrinolysis, 7, pp. 117-121
  • Massignon, D., Lepape, A., Bienvenu, J., Barbier, B., Boileau, C., Coeur, P., Coagulation/fibrinolysis balance in septic shock related to cytokines and clinical state (1994) Haemostas, 24, pp. 36-48
  • Philippe, J., Dooijewaard, G., Offner, F., Turion, P., Baele, G., Leroux-Roels, G., Granulocyte elastase, tumor necrosis factor-alpha and urokinase levels as prognostic markers in severe infection (1992) Thromb Haemost, 68, pp. 19-23
  • Nuijens, J.H., Abbink, J.J., Wachtfogel, Y.T., Colman, R.W., Eerenberg, A.J., Dors, D., Kamp, A.J., Hack, C.E., Plasma elastase alpha-1-antitrypsin and lactoferrin in sepsis: Evidence for neutrophils as mediators in fatal sepsis (1992) J Lab Clin Med, 119, pp. 159-168

Citas:

---------- APA ----------
Duboscq, C., Quintana, I., Bassilotta, E., Bergonzelli, G.E., Porterie, P., Sassetti, B., Haedo, A.S.,..., Kordich, L. (1997) . Plasminogen: An important hemostatic parameter in septic patients. Thrombosis and Haemostasis, 77(6), 1090-1095.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03406245_v77_n6_p1090_Duboscq [ ]
---------- CHICAGO ----------
Duboscq, C., Quintana, I., Bassilotta, E., Bergonzelli, G.E., Porterie, P., Sassetti, B., et al. "Plasminogen: An important hemostatic parameter in septic patients" . Thrombosis and Haemostasis 77, no. 6 (1997) : 1090-1095.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03406245_v77_n6_p1090_Duboscq [ ]
---------- MLA ----------
Duboscq, C., Quintana, I., Bassilotta, E., Bergonzelli, G.E., Porterie, P., Sassetti, B., et al. "Plasminogen: An important hemostatic parameter in septic patients" . Thrombosis and Haemostasis, vol. 77, no. 6, 1997, pp. 1090-1095.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03406245_v77_n6_p1090_Duboscq [ ]
---------- VANCOUVER ----------
Duboscq, C., Quintana, I., Bassilotta, E., Bergonzelli, G.E., Porterie, P., Sassetti, B., et al. Plasminogen: An important hemostatic parameter in septic patients. THROMB. HAEMOST. 1997;77(6):1090-1095.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03406245_v77_n6_p1090_Duboscq [ ]