Artículo

Ahmad, S.; Hewett, P.W.; Fujisawa, T.; Sissaoui, S.; Cai, M.; Gueron, G.; Al-Ani, B.; Cudmore, M.; Faraz Ahmed, S.; Wong, M.K.K.; Wegiel, B.; Otterbein, L.E.; Vítek, L.; Ramma, W.; Wang, K.; Ahmed, A. "Carbon monoxide inhibits sprouting angiogenesis and vascular endothelial growth factor receptor-2 phosphorylation" (2015) Thrombosis and Haemostasis. 113(2):329-337
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Abstract:

Carbon monoxide (CO) is a gaseous autacoid known to positively regulate vascular tone; however, its role in angiogenesis is unknown. The aim of this study was to investigate the effect of CO on angiogenesis and vascular endothelial growth factor (VEGF) receptor-2 phosphorylation. Human umbilical vein endothelial cells (HUVECs) were cultured on growth factor- reduced Matrigel and treated with a CO-releasing molecule (CORM-2) or exposed to CO gas (250 ppm). Here, we report the surprising finding that exposure to CO inhibits vascular endothelial growth factor (VEGF)-induced endothelial cell actin reorganisation, cell proliferation, migration and capillary-like tube formation. Similarly, CO suppressed VEGF-mediated phosphorylation of VEGFR-2 at tyrosine residue 1175 and 1214 and basic fibroblast growth factor- (FGF-2) and VEGF-mediated Akt phosphorylation. Consistent with these data, mice exposed to 250 ppm CO (1h/day for 14 days) exhibited a marked decrease in FGF-2-induced Matrigel plug angiogenesis (p<0.05). These data establish a new biological function for CO in angiogenesis and point to a potential therapeutic use for CO as an anti-angiogenic agent in tumour suppression. © Schattauer 2015.

Registro:

Documento: Artículo
Título:Carbon monoxide inhibits sprouting angiogenesis and vascular endothelial growth factor receptor-2 phosphorylation
Autor:Ahmad, S.; Hewett, P.W.; Fujisawa, T.; Sissaoui, S.; Cai, M.; Gueron, G.; Al-Ani, B.; Cudmore, M.; Faraz Ahmed, S.; Wong, M.K.K.; Wegiel, B.; Otterbein, L.E.; Vítek, L.; Ramma, W.; Wang, K.; Ahmed, A.
Filiación:Vascular and Obstetric Biology Laboratory, Aston Medical School, Aston University, Birmingham, United Kingdom
Department of Reproductive and Vascular Biology, Institute of Biomedical Research, College of Medicine and Dentistry, University of Birmingham, Birmingham, United Kingdom
Department of Biological Chemistry, School of Sciences, University of Buenos Aires, IQUIBICEN-CONICET, Buenos Aires, Argentina
School of Medicine, University of Liverpool, Liverpool, United Kingdom
University of Southern California, USC / Norris Comprehensive Cancer Center, Los Angeles, CA, United States
Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, United States
Charles University, Prague, Czech Republic
Palabras clave:Angiogenesis; Carbon monoxide; Endothelial cells; Vascular endothelial growth factor; Vascular endothelial growth factor receptor-2; actin; carbon monoxide; cytochrome c oxidase; fibroblast growth factor 2; protein kinase B; protein tyrosine phosphatase; tyrosine; vasculotropin receptor 2; carbon monoxide; collagen; drug combination; laminin; matrigel; organometallic compound; proteoglycan; recombinant protein; tricarbonyldichlororuthenium (II) dimer; tyrosine; vascular endothelial growth factor receptor-2, human; vasculotropin receptor 2; angiogenesis; angiogenesis assay; animal experiment; animal tissue; Article; cancer inhibition; cell migration assay; cell proliferation assay; controlled study; down regulation; drug mechanism; human; human cell; immunoprecipitation; mouse; nonhuman; priority journal; protein phosphorylation; umbilical vein endothelial cell; Western blotting; animal; C57BL mouse; cell motion; cell proliferation; chemistry; cytology; drug combination; endothelium cell; metabolism; neovascularization (pathology); phosphorylation; Animals; Carbon Monoxide; Cell Movement; Cell Proliferation; Collagen; Drug Combinations; Endothelial Cells; Human Umbilical Vein Endothelial Cells; Humans; Immunoprecipitation; Laminin; Mice, Inbred C57BL; Neovascularization, Pathologic; Organometallic Compounds; Phosphorylation; Proteoglycans; Recombinant Proteins; Tyrosine; Vascular Endothelial Growth Factor Receptor-2
Año:2015
Volumen:113
Número:2
Página de inicio:329
Página de fin:337
DOI: http://dx.doi.org/10.1160/TH14-01-0002
Título revista:Thrombosis and Haemostasis
Título revista abreviado:Thromb. Haemost.
ISSN:03406245
CODEN:THHAD
CAS:carbon monoxide, 630-08-0; cytochrome c oxidase, 72841-18-0, 9001-16-5; fibroblast growth factor 2, 106096-93-9; protein kinase B, 148640-14-6; protein tyrosine phosphatase, 79747-53-8, 97162-86-2; tyrosine, 16870-43-2, 55520-40-6, 60-18-4; collagen, 9007-34-5; laminin, 2408-79-9; matrigel, 119978-18-6; Carbon Monoxide; Collagen; Drug Combinations; Laminin; matrigel; Organometallic Compounds; Proteoglycans; Recombinant Proteins; tricarbonyldichlororuthenium (II) dimer; Tyrosine; Vascular Endothelial Growth Factor Receptor-2; vascular endothelial growth factor receptor-2, human
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03406245_v113_n2_p329_Ahmad

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Citas:

---------- APA ----------
Ahmad, S., Hewett, P.W., Fujisawa, T., Sissaoui, S., Cai, M., Gueron, G., Al-Ani, B.,..., Ahmed, A. (2015) . Carbon monoxide inhibits sprouting angiogenesis and vascular endothelial growth factor receptor-2 phosphorylation. Thrombosis and Haemostasis, 113(2), 329-337.
http://dx.doi.org/10.1160/TH14-01-0002
---------- CHICAGO ----------
Ahmad, S., Hewett, P.W., Fujisawa, T., Sissaoui, S., Cai, M., Gueron, G., et al. "Carbon monoxide inhibits sprouting angiogenesis and vascular endothelial growth factor receptor-2 phosphorylation" . Thrombosis and Haemostasis 113, no. 2 (2015) : 329-337.
http://dx.doi.org/10.1160/TH14-01-0002
---------- MLA ----------
Ahmad, S., Hewett, P.W., Fujisawa, T., Sissaoui, S., Cai, M., Gueron, G., et al. "Carbon monoxide inhibits sprouting angiogenesis and vascular endothelial growth factor receptor-2 phosphorylation" . Thrombosis and Haemostasis, vol. 113, no. 2, 2015, pp. 329-337.
http://dx.doi.org/10.1160/TH14-01-0002
---------- VANCOUVER ----------
Ahmad, S., Hewett, P.W., Fujisawa, T., Sissaoui, S., Cai, M., Gueron, G., et al. Carbon monoxide inhibits sprouting angiogenesis and vascular endothelial growth factor receptor-2 phosphorylation. Thromb. Haemost. 2015;113(2):329-337.
http://dx.doi.org/10.1160/TH14-01-0002