Artículo

La versión final de este artículo es de uso interno de la institución.
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Follicle-stimulating hormone (FSH) and luteinizing hormone (LH) expressing cells were detected in pituitary, brain and ovary of the Perciform cichlid fish Cichlasoma dimerus. This detection was carried out by immunohistochemistry (IHC) and Western blot techniques using antisera of the Cyprinodontiform Fundulus heteroclitus raised against the conservative region of the teleost βFSH and the βLH subunits. The estimated molecular weights were 24 kDa for LH and 19 and 15 kDa for FSH. In the adult pituitary, both cell types were distributed along mid and ventral zones of the proximal pars distalis (PPD, mid-immunoreactive cells), and along the ventral and dorsal external border of the pars intermedia (PI, high-immunoreactive cells). Double IHC showed that FSH and LH are mainly expressed in different pituitary cells. FSH cells were detected in the pituitary around day 21 after hatching (ah) (prior to sex differentiation), while LH cells were detected by day 60 ah (during the sexual differentiation period). A correlation between gonadal sex differentiation and FSH was demonstrated in a 15 days organ culture system. FSH and LH neurons were localized in the nucleus lateralis tuberis and their fibers project through the ventral hypothalamus, preoptic area and neurohypophysis. FSH neurons differentiated on day 21 ah, while LH neurons appeared on day 15 ah. In the ovary, the immunoreactivity for both FSH and LH was restricted to the cytoplasm of previtellogenic and early vitellogenic oocytes. © 2006 Springer-Verlag.

Registro:

Documento: Artículo
Título:Identification of immunoreactive FSH and LH cells in the cichlid fish Cichlasoma dimerus during the ontogeny and sexual differentiation
Autor:Pandolfi, M.; Lo Nostro, F.L.; Shimizu, A.; Pozzi, A.G.; Meijide, F.J.; Vazquez, G.R.; Maggese, M.C.
Filiación:Laboratorio de Embriología Animal, Departamento de Biodiversidad Y Biología Experimental, Pabellón 2, Buenos Aires C1428EHA, Argentina
Consejo Nacional de Investigaciones Y Tecnicas (CONICET), Departamento de Biodiversidad Y Biología Experimental, Pabellón 2, Buenos Aires C1428EHA, Argentina
National Research Institute of Fisheries Science, Fisheries Research Agency, Kanazawa, Yokohama 236-8648, Japan
Laboratorio de Biología del Desarrollo, Departamento de Biodiversidad Y Biología Experimental, Pabellón 2, Buenos Aires C1428EHA, Argentina
Palabras clave:Cichlidae; Gonadotropins; Ontogeny; Sexual differentiation; Teleostei; antiserum; follitropin; luteinizing hormone; animal cell; article; brain; cell type; cichlid; controlled study; Cyprinodontiformes; cytoplasm; female; follitropin release; hypophysis; hypophysis intermediate lobe; hypophysis pars distalis; hypothalamus; hypothalamus nucleus; immunocompetent cell; immunohistochemistry; luteinizing hormone release; male; molecular weight; nerve cell; neurohypophysis; nonhuman; ontogeny; organ culture; ovary; preoptic area; priority journal; sex differentiation; teleost; vitellogenesis; Western blotting; Age Factors; Animals; Brain; Cichlids; Female; Follicle Stimulating Hormone; Gonadotropin-Releasing Hormone; Immunohistochemistry; Luteinizing Hormone; Male; Nerve Fibers; Organ Culture Techniques; Ovary; Pituitary Gland; Sex Differentiation; Testis; Vitellogenesis; Cichlasoma; Cichlidae; Cyprinodontiformes; Fundulus heteroclitus; Perciformes; Teleostei
Año:2006
Volumen:211
Número:5
Página de inicio:355
Página de fin:365
DOI: http://dx.doi.org/10.1007/s00429-006-0086-0
Título revista:Anatomy and Embryology
Título revista abreviado:Anat. Embryol.
ISSN:03402061
CODEN:ANEMD
CAS:follitropin, 9002-68-0; luteinizing hormone, 39341-83-8, 9002-67-9; Follicle Stimulating Hormone, 9002-68-0; Gonadotropin-Releasing Hormone, 33515-09-2; Luteinizing Hormone, 9002-67-9
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03402061_v211_n5_p355_Pandolfi

Referencias:

  • Calman, B., Lin, Y.W., Wallace, R.A., Preparation and use of specific antibodies to the-I and-II subunits of gonadotropic hormone from Fundulus heteroclitus pituitary (2001) Gen Comp Endocrinol, 123, pp. 203-209
  • Croxatto, H., Arrau, J., Croxatto, H., Luteinizing hormone-like activity in human median eminence extracts (1964) Nature, 204, pp. 584-585
  • Demski, L.S., Sloan, H.E., A direct magnocellular-preopticospinal pathway in goldfish: Implications for control of sex behavior (1985) Neurosci Lett, 55, pp. 283-288
  • Dufour, S., Huang, Y.S., Rosseau, K., Puberty in teleosts: New insights into the role of peripheral signals in the stimulation of pituitary gonadotropins (2000) Proceedings of the VI International Symposium on Reproductive Physiology of Fish, pp. 455-461. , University of Bergen, Bergen
  • Emanuele, N., Oslapas, R., Connick, E., Kirsteins, L., Lawrence, A.M., Hypothalamic LH may play a role in control of pituitary LH release (1981) Neuroendocrinology, 33, pp. 12-17
  • Feist, G., Shreck, C.B., Brain-pituitary-gonadal axis during early development and sexual differentiation in the rainbow trout, Oncorhynchus mykiss (1996) Gen Comp Endocrinol, 102, pp. 394-409
  • Foran, C.M., Bass, A.H., Preoptic GnRH and AVT: Axes for sexual plasticity in teleosts fish (1999) Gen Comp Endocrinol, 116, pp. 141-152
  • Garcia-Ayala, A., Garcia-Hernandez, M.P., Quesada, J.A., Agulleiro, B., Gonadotropic and thyrotropic cells from the Mediterranean yellowtail (Seriola dumerilii; Risso, 1810): Immunocytochemical and ultrastructural characterization (1998) Anat Rec, 250, pp. 448-458
  • Ayala, G., Villaplana, M., García Hernández, M.P., Chaves Pozo, E., Agulleiro, B., FSH-, LH- and TSH-expressing cells during development of Sparus aurata L. (Telesotei). An immunocytochemical study (2003) Gen Comp Endocrinol, 134, pp. 72-79
  • Gross, D.S., Page, R.B., Luteinizing hormone and follicle stimulating hormone production in the pars tuberalis of hypophysectomized rats (1979) Am J Anat, 156, pp. 285-291
  • Hojvat, S., Emanuele, N., Baker, G., Connick, E., Kirsteins, L., Lawrence, A.M., Growth hormone (GH), thyroid-stimulating hormone (TSH), and luteinizing hormone (LH)-like peptides in the rodent brain: Non-parallel ontogenic development with pituitary counterparts (1982) Dev Brain Res, 4, pp. 427-434
  • Hostetter, G., Gallo, R.V., Brownfield, M.S., Presence of immunoreactive luteining hormone in the rat forebrain (1981) Neuroendocrinology, 33, pp. 241-245
  • Ito, R., Abe, S.I., FSH-initiated differentiation of newt spermatogonia to primary spermatocytes in germ-somatic cell reaggregates cultured within a collagen matrix (1999) Int J Dev Biol, 43 (2), pp. 111-116
  • Kagawa, H., Kawazoe, I., Tanaka, H., Okuzawa, K., Immunocytochemical identification of two distinct gonadotropic cells (GTH I and GTH II) in the pituitary of bluefin tuna, Thunnus thynnus (1998) Gen Comp Endocrinol, 110, pp. 11-18
  • Kawakami, M., Sakuma, Y., Responses of hypothalamic neurons to the microiontophoresis of LHRH, LH, and FSH under various levels of circulating ovarian hormones (1974) Neuroendocrinology, 15, pp. 290-307
  • Maekawa, K., Ji, Z.S., Abe, S., Proliferation of newt spermatogonia by mammalian FSH via Sertoli cells in vitro (1995) J Exp Zool, 272, pp. 363-373
  • Magliulo-Cepriano, L., Schreibman, M.P., Blüm, V., Distribution of variant forms of immunoreactive gonadotropin-releasing hormone and beta-gonadotropins I and II in the platyfish, Xiphophorus maculatus, from birth to sexual maturity (1994) Gen Comp Endocrinol, 94, pp. 135-150
  • Mal, A.O., Swanson, O., Dickhoff, W.W., Immunocytochemistry of the developing salmon pituitary gland (1989) Am Zool, 29, pp. 94A
  • Meijide, F.J., Lo Nostro, F.L., Guerrero, G.A., Gonadal development and sex differentiation in the cichlid fish Cichlasoma dimerus (Teleostei, Perciformes): A light- and electron-microscopic study (2005) J Morphol, 264, pp. 191-210
  • Miranda, L.A., Strüssmann, C.A., Somoza, G.M., Immunocytochemical identification of GtH1 and GtH2 cells during the temperature-sensitive period for sex differentiation in pejerrey, Odontesthes bonariensis (2001) Gen Comp Endocrinol, 124, pp. 45-52
  • Nozaki, M., Naito, N., Swanson, P., Dickhoff, W.W., Nakai, Y., Suzuki, K., Kawauchi, H., Salmonid pituitary gonadotrophs. II. Ontogeny of GTH I and GTH II cells in the rainbow trout (Salmo gairdneri irideus) (1990) Gen Comp Endocrinol, 77, pp. 358-367
  • Pacold, S.T., Kirsteins, L., Hojvat, S., Lawrence, A.M., Hagen, T.C., Biologically active pituitary hormones in the rat brain amygdaloid nucleus (1978) Science, 199, pp. 804-806
  • Pandolfi, M., Paz, D.A., Maggese, M.C., Meijide, F.J., Vissio, P.G., Immunocytochemical localization of different cell types in the adenohypophysis of Cichlasoma dimerus (Teleostei, Perciformes) (2001) Biocell, 25, pp. 35-42
  • Pandolfi, M., Paz, D.A., Maggese, M.C., Ravaglia, M.A., Vissio, P.G., Ontogeny of immunoreactive somatolactin, prolactin and growth hormone secretory cells in the developing pituitary gland of Cichlasoma dimerus (Teleostei, Cichlidae) (2001) Anat Embryol, 203, pp. 461-468
  • Pandolfi, M., Parhar, I.S., Ravaglia, M.A., Meijide, F.J., Maggese, M.C., Paz, D.A., Ontogeny and distribution of gonadotropin-releasing hormone (GnRH) neuronal systems in the brain of the cichlid fish Cichlasoma dimerus (2002) Anat Embryol, 205, pp. 271-281
  • Pandolfi, M., Muñoz Cueto, J.A., Lo Nostro, F.L., Downsp, P.J.L., Paz, D.A., Maggese, M.C., Urbanski, H., The GnRH systems of Cichlasoma dimerus (Perciformes, Cichlidae) revisited: A localization and developmental study using antibodies and riboprobes to the GnRH- Associated peptides (2005) Cell and Tiss Res, 321, pp. 219-232
  • Parhar, I.S., Iwata, M., Pfaff, D.W., Schwanzel-Fukuda, M., Embryonic development of gonadotropin-releasing hormone neurones in the sockeye salmon (1995) J Comp Neurol, 362, pp. 256-270
  • Parhar, I.S., Soga, T., Ogawa, S., Sakuma, Y., FSH and LH-β subunits in the preoptic nucleus: Ontogenic expression in teleost (2003) Gen Comp Endocrinol, 132, pp. 369-378
  • Quesada, J., Lozano, M.T., Ortega, A., Agulleiro, B., Immunocytochemical and ultrastructural characterization of the cell types in the adenohypophysis of Sparus aurata L. Teleost (1988) Gen Comp Endocrinol, 72, pp. 209-225
  • Saga, T., Oota, Y., Nozaki, M., Swanson, P., Salmonid pituitary gonadotrophs. III. Chronological appearence of GTH I and other adenohypophysial hormones in the pituitary of the developing rainbow trout (Oncorhynchus mykiss irideus) (1993) Gen Comp Endocrinol, 92, pp. 233-241
  • Saga, T., Yamaki, K., Doi, Y., Yoshizuka, M., Chronological study of the appearance of adenohyphophysial cells in the ayu (Plecoglossus altivelis) (1999) Anat Embryol, 200, pp. 469-475
  • Schreibman, M.P., Margolis-Kazan, H., Halpern-Sebold, L., Immunoreactive gonadotropin and luteinizing hormone releasing hormone in the pituitary gland of neonatal platyfish (1982) Gen Comp Endocrinol, 47, pp. 385-391
  • Segura-Noguera, M.M., Laiz-Carrión, L., Del Río, M.P., Mancera, J.M., An immunocytochemical study of the pituitary gland of the white seabream (Diplodus sargus) (2000) Histochem J, 32, pp. 733-742
  • Shimizu, A., Yamashita, M., Purification of mummichog (Fundulus heteroclitus) gonadotropins and their subunits, using an immunochemical assay with antisera raised against synthetic peptides (2002) Gen Comp Endocrinol, 125, pp. 79-91
  • Shimizu, A., Tanaka, H., Kagawa, H., Immunocytochemical applications of specific antisera raised against synthetic fragment peptides of mummichog GtH subunits: Examining seasonal variations of gonadotrophs (FSH cells and LH cells) in the mummichog and applications to other acanthopterygian fishes (2003) Gen Comp Endocrinol, 132, pp. 35-45
  • Shimizu, A., Sakai, T., Nashida, K., Kagawa, H., Universal antisera for immunocytochemical identification of two different gonadotrophs in acanthoptherygian fishes (2003) Fish Physiol Biochem, 29, pp. 275-287
  • Suzuki, K., Kawauchi, H., Nagahama, Y., Isolation and characterization of two distinct gonadotropins from chum salmon pituitary glands (1988) Gen Comp Endocrinol, 71, pp. 292-301
  • Terasawa, E.I., Whitmoyer, D.I., Sawyer, C.H., Effects of luteinizing hormone on multiple-unit activity in the rat hypothalamus (1969) Am J Physiol, 217, pp. 1119-1126
  • Tyler, C.R., Sumpter, J.P., Kawauchi, H., Swanson, P., Involvement of gonadotropin in the uptake of vitellogenin into vitellogenic oocytes of the rainbow trout, (Oncorhynchus mykiss) (1991) Gen Comp Endocrinol, 84, pp. 291-299
  • Van Der Hurk, R., Effects of steroids on gonadotropic (GtH) cells in the pituitary of rainbow trout, Salmo gairdneri, shortly after hatching (1982) Cell Tiss Res, 224, pp. 361-368
  • Van Winkoop, A., Timmermans, L.P.M., Booms, G.H.R., The expression of germ cell differentiation antigens, as defined with monoclonal antibodies, in correlation with the ontogeny of gonadotropic cells in the hypophysis of carp (1987) Proceedings of the Third International Symposium on the Reproductive Physiology of Fish, p. 222. , DR Ilder, LW Crim, Jm Walsh (eds) St Johńs, New Foundland, Canada
  • Vissio, P.G., Paz, D.A., Maggese, M.C., The adenohypophysis of the swamp eel, Synbranchus marmoratus, an immunocytochemical analysis (1996) Biocell, 20, pp. 155-161
  • Wong, T., Zohar, Y., Novel expression of gonadotropin subunit genes in oocytes of the gilthead seabream (Sparus aurata) (2004) Endocrinology, 145, pp. 5210-5220
  • Yaron, Z., Gur, G., Melamed, P., Rosenfeld, H., Levavi-Sivan, B., Elizur, A., Regulation of gonadotropin subunit genes in tilapia (2001) Comp Biochem Physiol Part B, 129, pp. 489-502
  • Yazawa, T., Yamamoto, T., Jin, Y., Abe, S., Follicle-stimulating hormone is indispensable for the last spermatogonial mitosis preceding meiosis initiation in newts (Cynops pyrrhogaster) (2002) Biol Reprod, 66, pp. 14-20
  • Yoshiura, Y., Suetake, H., Aida, K., Duality of gonadotropin in a primitive teleost, Japanese eel (Anguilla japonica) (1999) Gen Comp Endocrinol, 114, pp. 121-131

Citas:

---------- APA ----------
Pandolfi, M., Lo Nostro, F.L., Shimizu, A., Pozzi, A.G., Meijide, F.J., Vazquez, G.R. & Maggese, M.C. (2006) . Identification of immunoreactive FSH and LH cells in the cichlid fish Cichlasoma dimerus during the ontogeny and sexual differentiation. Anatomy and Embryology, 211(5), 355-365.
http://dx.doi.org/10.1007/s00429-006-0086-0
---------- CHICAGO ----------
Pandolfi, M., Lo Nostro, F.L., Shimizu, A., Pozzi, A.G., Meijide, F.J., Vazquez, G.R., et al. "Identification of immunoreactive FSH and LH cells in the cichlid fish Cichlasoma dimerus during the ontogeny and sexual differentiation" . Anatomy and Embryology 211, no. 5 (2006) : 355-365.
http://dx.doi.org/10.1007/s00429-006-0086-0
---------- MLA ----------
Pandolfi, M., Lo Nostro, F.L., Shimizu, A., Pozzi, A.G., Meijide, F.J., Vazquez, G.R., et al. "Identification of immunoreactive FSH and LH cells in the cichlid fish Cichlasoma dimerus during the ontogeny and sexual differentiation" . Anatomy and Embryology, vol. 211, no. 5, 2006, pp. 355-365.
http://dx.doi.org/10.1007/s00429-006-0086-0
---------- VANCOUVER ----------
Pandolfi, M., Lo Nostro, F.L., Shimizu, A., Pozzi, A.G., Meijide, F.J., Vazquez, G.R., et al. Identification of immunoreactive FSH and LH cells in the cichlid fish Cichlasoma dimerus during the ontogeny and sexual differentiation. Anat. Embryol. 2006;211(5):355-365.
http://dx.doi.org/10.1007/s00429-006-0086-0