Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Electron microscopy of the lungs of Melanophryniscus stelzneri stelzneri (Anura) revealed the presence of a complex pattern of corpuscular cells (CCs). The respiratory surface over the septa presents small areas where the CCs are grouped forming neuroepithelial bodies (NEBs). These corpuscular structures can also be localized in the inner layer of the lung wall. Although in both cases NEBs protrude slightly into the airway lumen, they are separated from the airway lumen and the basal connective tissue by thin apical and basal cytoplasmic processes of neighbouring pneumocytes. Ultrastructurally, the CCs possess a large nucleus, clear cytoplasm and vesicles of variable morphology and size, containing an electron dense material surrounded by a lucent space in some cases. The size of these dense-core vesicles (DCVs) ranged from 40 to 100 nm. The NEBs are associated with afferent and efferent terminal nerves. These types of nerve endings are located between the CCs and in the basal part of the NEBs. The location of the NEBs in strategic positions on the septa and in the wall of the lung, the presence of the DCVs in the basolateral region of the CCs, the occurrence of synaptic contacts between nerve endings and the CCs and the occurrence of capillaries close to the NEBs, suggest a receptosecretory function for NEBs in the lung of M.s. stelzneri.

Registro:

Documento: Artículo
Título:Neuroepithelial bodies in the lung of Melanophryniscus stelzneri stelzneri (Anura, Bufonidae)
Autor:Hermida, G.N.; Farías, A.; Fiorito, L.E.
Filiación:Lab. de Histología Animal, Microanatomía de Anfibios, Ciudad Universitaria (C1428 EHA), Av. Güiraldes s/n, Buenos Aires, Argentina
Palabras clave:Amphibia; Dense-core vesicles; Electron microscopy; Neuroendocrine system; Respiratory organ; airway; animal cell; animal tissue; article; controlled study; efferent nerve; electron microscopy; frogs and toads; gene location; lung alveolus epithelium; melanophryniscus stelzneri stelzneri; nerve ending; neuroepithelium; nonhuman; priority journal; sensory nerve conduction; Amphibia; Animalia; Anura; Bufonidae; Melanophryniscus; Melanophryniscus stelzneri
Año:2003
Volumen:207
Número:4-5
Página de inicio:373
Página de fin:379
DOI: http://dx.doi.org/10.1007/s00429-003-0353-2
Título revista:Anatomy and Embryology
Título revista abreviado:Anat. Embryol.
ISSN:03402061
CODEN:ANEMD
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03402061_v207_n4-5_p373_Hermida

Referencias:

  • Adriaensen, D., Scheuermann, D.W., Neuroendocrine cells and nerves of the lung (1993) Anat Rec, 236, pp. 70-85
  • Adriaensen, D., Brouns, I., Van Genechten, J., Timmermans, J.P., Functional morphology of pulmonary neuroepithelial bodies: Extremely complex airway receptors (2003) Anat Rec, 270, pp. 25-40
  • Bodegas, M.E., Montuenga, L.M., Polak, J.M., Sesma, P., Immunohistochemical colocalization of 7 B2 and 5HT in the neuroepithelial bodies of the lung of Rana temporaria (1993) Cell Tiss Res, 273, pp. 137-140
  • Cook, R.D., King, A.S., A neurite-receptor complex in the avian lung: Electron microscopical observations (1969) Experientia, 25, pp. 1162-1164
  • Cutz, E., Goniakowska-Witalińska, L., Chan, W., An immunohistochemical study of regulatory peptides in lungs of amphibians (1986) Cell Tiss Res, 244, pp. 227-233
  • Goniakowska-Witalińska, L., Neuroepithelial bodies in the lung of the tree frog, Hyla arborea, L. A scannning and transmission electron microscopy study (1981) Cell Tiss Res, 210, pp. 521-524
  • Goniakowska-Witalińska, L., Lung of the tree frog, Hyla arborea L. A scanning and transmission elctron microscopic study (1986) Anat Embryol, 174, pp. 379-389
  • Goniakowska-Witalińska, L., Cutz, E., Ultrastructure of neuroendocrine cells in the lungs of three anuran species (1990) J Morphol, 203, pp. 1-9
  • Goniakowska-Witalińska, L., Lauweryns, J.M., Van Ranst, L., Neuroepithelial bodies in the lungs of Bombina orientalis (Boul.) (1990) Cell Tiss Res, 273, pp. 137-140
  • Goniakowska-Witalińska, L., Lauweryns, J.M., Zaccone, G., Fasulo, S., Tagliafierro, G., Ultrastructural and immunocytochemistry of the neuroepithelial bodies in the lung of the tiger salamander, Ambystoma tigrinum (Urodela, Amphibia) (1992) Anat Rec, 234, pp. 419-431
  • Goniakowska-Witalińska, L., Neuroepithelial bodies and solitary neuroendocrine cell in the lungs of amphibia (1997) Microsc Res Technol, 37, pp. 13-30
  • Hermida, G.N., Farias, A., Fiorito, L.E., Ultrastructural characteristics of the lung of Melanophryniscus stelzneri stelzneri (Weyenberg, 1875) (Anura, Bufonidae) (2002) Biocell, 26 (3), pp. 347-355
  • Lauweryns, J.M., Peuskens, J.C., Neuro-epithelial bodies (neuroreceptor or secretory organs?) in human infant bronchial and bronchiolar epithelium (1972) Anat Rec, 172, pp. 471-482
  • Lauweryns, J.M., Cokelaere, M., Theunynck, P., Neuroepithelial bodies in the respiratory mucosa of various mammals (1972) Z Zellforsch, 135, pp. 569-592
  • Lauweryns, J.M., Cokelaere, M., Deleersnyder, M., Liebens, M., Intrapulmonary neuro-epithelial bodies in newborn rabbits. Influence of hypoxia, hyperoxia, hypercapnia, nicotine, reserpine, L-DOPA and 5-HTP (1977) Cell Tiss Res, 182, pp. 425-440
  • Lauweryns, J.M., Van Lommel, A., Ultrastructure of nerve endings and synaptic junctions in rabbit intrapulmonary neuroepithelial bodies; a single and serial section analysis (1987) J Anat., 151, pp. 65-83
  • Matsumura, H., Electron microscopic studies of the lung of the salamander, Hynobius nebulosus. III. A scanning and transmission electron microscopic observations on neuroepithelial bodies (1985) Okajimas Folia Anat Jpn, 62, pp. 187-204
  • Pastor, L.M., Ballesta, J., Castells, M.T., Perez-Tomas, R., Marin, J.A., Madrid, J.F., A microscopic study of the lung of Testudo graeca (Chelonia) (1989) J Anat, 164, pp. 19-39
  • Reynolds, E.S., The use of lead citrate at high Ph as an electron-opaque stain in electron microscopy (1963) J Cell Biol, 17, pp. 208-212
  • Rogers, D.C., Haller, C.J., Innervation and cytochemistry of the neuroepithelial bodies in the ciliated epithelium of the toad lung (Bufo marinus) (1978) Cell Tiss Res, 195, pp. 395-410
  • Rogers, D.C., Haller, C.J., The ultrastructural characteristics of the apical cell in the neuroepithelial bodies of the toad lung (Bufo marinus) (1980) Cell Tiss Res, 209, pp. 485-498
  • Scheuermann, D.W., De Groodt-Lasseel, M.H.A., Stilman, C., Meisters, M.L., A correlative light, fluorescence and electron microscopic study of neuroepithelial bodies in the lung of the red eared turtle Pseudemis scripta elegans (1983) Cell Tiss Res, 234, pp. 249-269
  • Scheuermann, D.W., Morphology and cytochemistry of the endocrine epithelial system in the lung (1987) Int Rev Cytol, 106, pp. 35-88
  • Scheuermann, D.W., Adriaensen, D., Timmermans, J.P., Groodt-Lasseel, M.H.A., Neuroepithelial endocrine cells in the lungs of Ambystoma mexicanum (1989) Anat Rec, 225, pp. 139-149
  • Scheuermann, D.W., Comparative histology of pulmonary neuroendocrine cell system in mammalian lungs (1997) Microsc Res Technol, 37, pp. 31-42
  • Sorokin, S.P., Hoyt, R.F., Neuroepithelial bodies and solitary small-granule cells (1989) Lung Cell Biology, pp. 191-344. , Massaro D (ed) Dekker, New York Basel
  • Van Lommel, A., Lauweryns, J.M., Neuroepithelial bodies in the fawn hooded rat lung: Morphological and neuroanatomical evidence for a sensory innervation (1993) J Anat, 183, pp. 553-566
  • Van Lommel, A., Lauweryns, J.M., Ultrastructure and innervation of neuroepithelial bodies in the lungs of newborn cats (1993) Anat Rec, 236, pp. 181-190
  • Van Lommel, A., Lauweryns, J.M., Berthound, H.R., Pulmonary neuroepithelial bodies are innervated by vagal afferent nerves: An investigation with in vivo anterograde Dil tracing and confocal microscopy (1998) Anat Embryol, 197, pp. 325-330
  • Van Lommel, A., Bollé, T., Fannes, W., Lauweryns, J.M., The pulmonary neuroendocrine system: The past decade (1999) Arch Histol Cytol, 62 (1), pp. 1-16
  • Wasano, K., Yamamoto, T., Monoamine-containing granulated cells in the frog lung (1978) Cell Tiss Res, 193, pp. 201-209
  • Youngston, C., Nurse, H., Yeger, H., Cutz, E., Oxygen sensing in airway chemoreceptors (1993) Nature, 365, pp. 153-155
  • Zaccone, G., Goniakowska-Witalińska, L., Lauweryns, J.M., Fasulo, S., Fine structure and serotonin immunohistochemistry of the neuroendocrine cells in the lungs of bichirs Polypterus delhezi and P. ornatipinnis (1989) Bas Appl Histochem., 33, pp. 277-287
  • Zaccone, G., Tagliafierro, L., Goniakowska-Witalińska, L., Fasulo, S., Ainis, L., Mauceri, A., Serotonin-like immunoreactive cells in the pulmonary epithelium of ancient fish species (1989) Histochem, 92, pp. 61-63

Citas:

---------- APA ----------
Hermida, G.N., Farías, A. & Fiorito, L.E. (2003) . Neuroepithelial bodies in the lung of Melanophryniscus stelzneri stelzneri (Anura, Bufonidae). Anatomy and Embryology, 207(4-5), 373-379.
http://dx.doi.org/10.1007/s00429-003-0353-2
---------- CHICAGO ----------
Hermida, G.N., Farías, A., Fiorito, L.E. "Neuroepithelial bodies in the lung of Melanophryniscus stelzneri stelzneri (Anura, Bufonidae)" . Anatomy and Embryology 207, no. 4-5 (2003) : 373-379.
http://dx.doi.org/10.1007/s00429-003-0353-2
---------- MLA ----------
Hermida, G.N., Farías, A., Fiorito, L.E. "Neuroepithelial bodies in the lung of Melanophryniscus stelzneri stelzneri (Anura, Bufonidae)" . Anatomy and Embryology, vol. 207, no. 4-5, 2003, pp. 373-379.
http://dx.doi.org/10.1007/s00429-003-0353-2
---------- VANCOUVER ----------
Hermida, G.N., Farías, A., Fiorito, L.E. Neuroepithelial bodies in the lung of Melanophryniscus stelzneri stelzneri (Anura, Bufonidae). Anat. Embryol. 2003;207(4-5):373-379.
http://dx.doi.org/10.1007/s00429-003-0353-2