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Abstract:

Bacterial artificial chromosome - fluorescence in situ hybridization (BAC-FISH) and cyclingprimed in situ labeling (C-PRINS) techniques were evaluated for integration of physical and genetic maps of sunflower (Helianthus annuus L.). Single-site SSR markers were selected from three linkage groups of a high-density sunflower genetic map. This selection was based on previously identified QTL associated to S. sclerotiorum. These markers were used to select BACs contaning single copy sequences for BAC-FISH aplication. Blocking of highly dispersed repetitive sunflower sequences reduced unspecific hybridization, and allowed the detection of specific signals for BACs containing SSR markers HA4222 and HA2600, anchored to LG 16 and LG 10, respectively. Single-site FISH signal detection was optimized by adjusting the relative quantity and quality of unlabelled repetitive sequences present in the blocking DNA. The SSR marker ORS1247 anchored to the LG 17 was detected by C-PRINS, which yielded fluorescence signals that were specific and intense. This progress in localizing single-copy sequences using BAC-FISH and indirect CPRINS strategies in sunflower will facilitate the integration of genetic and physical maps, allowing the identification of chromosomes containing key genes and/or QTL associated to agronomic important traits in sunflower.

Registro:

Documento: Artículo
Título:Detection of single copy sequences using BAC-FISH and C-PRINS techniques in sunflower chromosomes
Autor:Talia, P.; Greizerstein, E.J.; Hopp, H.E.; Paniego, N.; Poggio, L.; Heinz, R.A.
Filiación:Instituto de Biotecnología, CICVyA, INTA Castelar, N. Repetto y Los Reseros s/n, 1686 Hurlingham, Provincia de Buenos Aires, Argentina
Departamento de Ecología, Genética y Evolución and Departamento de Fisiología, Biologia Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellon II, C1428EHA, Buenos Aires, Argentina
Palabras clave:BAC-FISH; C-PRINS; Physical mapping; Single-copy sequences; Sunflower; plant DNA; article; bacterial artificial chromosome fluorescence in situ hybridization; chromosome identification; controlled study; cycling primed in situ labeling; fluorescence in situ hybridization; gene mapping; genetic linkage; genetic marker; nonhuman; nucleotide repeat; nucleotide sequence; plant chromosome; primed in situ labeling; process optimization; quantitative trait locus; Sclerotinia sclerotiorum; sequence analysis; signal detection; simple sequence repeat; sunflower; bacterial artificial chromosome; DNA sequence; genetics; methodology; Bacteria (microorganisms); Helianthus; Helianthus annuus; Base Sequence; Chromosomes, Artificial, Bacterial; Chromosomes, Plant; Genetic Markers; Helianthus; In Situ Hybridization, Fluorescence; Quantitative Trait Loci; Sequence Analysis, DNA
Año:2011
Volumen:35
Número:1
Página de inicio:19
Página de fin:28
Título revista:Biocell
Título revista abreviado:Biocell
ISSN:03279545
CODEN:BOCEE
CAS:Genetic Markers
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v35_n1_p19_Talia

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Citas:

---------- APA ----------
Talia, P., Greizerstein, E.J., Hopp, H.E., Paniego, N., Poggio, L. & Heinz, R.A. (2011) . Detection of single copy sequences using BAC-FISH and C-PRINS techniques in sunflower chromosomes. Biocell, 35(1), 19-28.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v35_n1_p19_Talia [ ]
---------- CHICAGO ----------
Talia, P., Greizerstein, E.J., Hopp, H.E., Paniego, N., Poggio, L., Heinz, R.A. "Detection of single copy sequences using BAC-FISH and C-PRINS techniques in sunflower chromosomes" . Biocell 35, no. 1 (2011) : 19-28.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v35_n1_p19_Talia [ ]
---------- MLA ----------
Talia, P., Greizerstein, E.J., Hopp, H.E., Paniego, N., Poggio, L., Heinz, R.A. "Detection of single copy sequences using BAC-FISH and C-PRINS techniques in sunflower chromosomes" . Biocell, vol. 35, no. 1, 2011, pp. 19-28.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v35_n1_p19_Talia [ ]
---------- VANCOUVER ----------
Talia, P., Greizerstein, E.J., Hopp, H.E., Paniego, N., Poggio, L., Heinz, R.A. Detection of single copy sequences using BAC-FISH and C-PRINS techniques in sunflower chromosomes. Biocell. 2011;35(1):19-28.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v35_n1_p19_Talia [ ]