Artículo
Julianelli, V.L.; Guerra, L.N.; Calvo, J.C. "Cell-cell communication between mouse mammary epithelial cells and 3T3-L1 preadipocytes: Effect on triglyceride accumulation and cell proliferation" (2007) Biocell. 31(2):237-245
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Abstract:
Interaction between parenchyma and stroma is essential for organogenesis, morphogenesis, and differentiation. Mammary gland has being the chosen model for developmental biologist because the most striking changes in morphology and function take place after birth. We have demonstrated a regulation of triglyceride accumulation by protein factors synthesized by normal mouse mammary gland epithelial cells (NMMG), acting on a cell line, 3T3-L1, long used as a model for adipogenesis. In this paper, we demonstrate that this inhibitory effect seems to be shared by other cells of epithelial origin but not by other cell types. We found a regulation of cell proliferation when NMMG cells are cultured in the presence of conditioned media from Swiss 3T3 or 3T3-L1 cells. We found a possible point of regulation for the mammary factor on a key enzyme of the lipid metabolic pathway, the glycerol-3-phosphate dehydrogenase. The inhibitory factor seems to have an effect on this enzyme's activity and reduces it. The results presented herein contribute to the understanding of cell-cell communication in a model of a normal mammary gland.
Registro:
| Documento: | Artículo |
| Título: | Cell-cell communication between mouse mammary epithelial cells and 3T3-L1 preadipocytes: Effect on triglyceride accumulation and cell proliferation |
| Autor: | Julianelli, V.L.; Guerra, L.N.; Calvo, J.C. |
| Filiación: | Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Ciudad Universitaria, (1428) Buenos Aires, Argentina IBYME, CONICET, Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina |
| Palabras clave: | Differentiation; Mammary epithelium; Triglycerides; glycerol 3 phosphate dehydrogenase; triacylglycerol; animal cell; article; breast epithelium; cell communication; cell proliferation; cell strain 3T3; cell type; controlled study; enzyme activity; human; human cell; mouse; nonhuman; proadipocyte; 3T3 Cells; 3T3-L1 Cells; Adipocytes, White; Animals; Cell Communication; Cell Differentiation; Cell Proliferation; Cells, Cultured; Culture Media, Conditioned; Epithelial Cells; Hela Cells; Humans; Mammary Glands, Animal; Mice; Triglycerides |
| Año: | 2007 |
| Volumen: | 31 |
| Número: | 2 |
| Página de inicio: | 237 |
| Página de fin: | 245 |
| Título revista: | Biocell |
| Título revista abreviado: | Biocell |
| ISSN: | 03279545 |
| CODEN: | BOCEE |
| CAS: | glycerol 3 phosphate dehydrogenase, 9001-49-4; Culture Media, Conditioned; Triglycerides |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v31_n2_p237_Julianelli |
Referencias:
- Calvo, J.C., Chernick, S., Rodbard, D., Mouse mammary epithelium produces a soluble heat-sensitive macromolecule that inhibits differentiation of 3T3-L1 preadipocytes (1992) Proc Soc Exp Biol Med, 201, pp. 174-179
- Deb, S., Amin, S., lmir, A.G., Yilmaz, M.B., Suzuki, T., Sasano, H., Bulun, S.E., Estrogen regulates expression of tumor necrosis factor receptors in breast adipose fibroblasts (2004) J Clin Endocrinol Metab, 89, pp. 4018-4024
- Hauner, H., Entenmann, G., Wabitsch, M., Gaillard, D., Ailhaud, G., Negrel, R., Pfeiffer, E.F., Promoting effect of glucocorticoids on the differentiation of human adipocyte precursor cells cultured in a chemically defined medium (1989) J Clin Invest, 84, pp. 1663-1670
- Hovey, R.C., McFadden, T.B., Akers, R.M., Regulation of Mammary Gland Growth and Morphogenesis by the Mammary Fat Pad: A Species Comparison (1999) J Mammary Gland Biol Neoplasia, 4, pp. 53-68
- Levine, J.F., Stockdale, F.E., Cell-Cell Interactions Promote Mammary Epithelial Cell Differentiation (1985) J Cell Biol, 100, pp. 1415-1422
- Meng, L., Zhou, J., Sasano, H., Suzuki, T., Zeitoun, K.M., Bulun, S.E., Tumor Necrosis Factor a and Interleukin 11 Secreted by Malignant Breast Epithelial Cells Inhibit Adipocyte Differentiation by Selectively Down-Regulating CCAAT/Enhancer Binding Protein a and Peroxisome Proliferator-activated Receptor g: Mechanism of Desmoplastic Reaction (2001) Cancer Res, 61, pp. 2250-2255
- Nichols, J.E., Bulun, S.E., Simpson, E.R., Effects of conditioned medium from different cultured cell types on aromatase expression in adipose stromal cells (1995) J Soc Gynecol Invest, 2, pp. 45-50
- Owens, R.B., Glandular epithelial cells from mice: A method for selective cultivation (1974) J Nat Cancer Inst, 52, pp. 1375-1378
- Owens, R.B., Smith, H.S., Hackett, A.J., Epithelial cells cultures from normal glandular tissue of mice (1974) J Nat Cancer Inst, 53, pp. 261-269
- Pagano, E., Calvo, J.C., ErbB2 and EGFR are downmodulated during the differentiation of 3T3-L1 preadipocytes (2003) J Cell Biochem, 90, pp. 561-572
- Qiu, Z., Wei, Y., Chen, N., Jiang, M., Wu, J., Liao, K., DNA synthesis and mitotic clonal expansion is not a required step for 3T3-L1 preadipocyte differentiation into adipocytes (2001) J Biol Chem, 276, pp. 11988-11995
- Wise, L., Green, H., Participation of one of cytosolic glycerolphosphate dehydrogenase in the adipose conversion of 3T3 cells (1978) J Biol Chem, 254, pp. 273-275
- Zizola, C.F., Balañá, M.E., Sandoval, M., Calvo, J.C., Changes in IGF-1 receptor and IGF-1 mRNA during differentiation of 3T3-L1 preadipocytes (2002) Biochimie, 84, pp. 975-980
Citas:
---------- APA ----------
Julianelli, V.L., Guerra, L.N. & Calvo, J.C. (2007) . Cell-cell communication between mouse mammary epithelial cells and 3T3-L1 preadipocytes: Effect on triglyceride accumulation and cell proliferation. Biocell, 31(2), 237-245.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v31_n2_p237_Julianelli [ ]
---------- CHICAGO ----------
Julianelli, V.L., Guerra, L.N., Calvo, J.C. "Cell-cell communication between mouse mammary epithelial cells and 3T3-L1 preadipocytes: Effect on triglyceride accumulation and cell proliferation" . Biocell 31, no. 2 (2007) : 237-245.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v31_n2_p237_Julianelli [ ]
---------- MLA ----------
Julianelli, V.L., Guerra, L.N., Calvo, J.C. "Cell-cell communication between mouse mammary epithelial cells and 3T3-L1 preadipocytes: Effect on triglyceride accumulation and cell proliferation" . Biocell, vol. 31, no. 2, 2007, pp. 237-245.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v31_n2_p237_Julianelli [ ]
---------- VANCOUVER ----------
Julianelli, V.L., Guerra, L.N., Calvo, J.C. Cell-cell communication between mouse mammary epithelial cells and 3T3-L1 preadipocytes: Effect on triglyceride accumulation and cell proliferation. Biocell. 2007;31(2):237-245.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v31_n2_p237_Julianelli [ ]
