Artículo

Este artículo es de Acceso Abierto y puede ser descargado en su versión final desde nuestro repositorio
Consulte la política de Acceso Abierto del editor

Abstract:

Prior to this work, we found that adrenal as well as extra-adrenal factors activate the response of renal l 11β-hydroxysteroid dehydrogenase 2 to stressful situations. These results -showing ways through which the organism hinders the pathological occupation of mineralocorticoid receptors by glucocorticoids leading to sodium retention and hypertension- prompted the present study on the nature of the above-mentioned extra-adrenal factors. Serotonin was chosen because of its properties as a widely distributed neurohormone, known to interact with glucocorticoids at many sites, also exhibiting increased levels and effects under stressful situations. We studied serotonin effects on 11β-hydroxysteroid dehydrogenase 2 activity in a cell line derived from distal nephron polarized-epithelium, employing 3H-corticosterone as substrate. The end-product, 3H-11- dehydrocorticosterone was separated from the substrate by HPLC and quantified. Serotonin stimulated 11β-hydroxysteroid dehydrogenase 2 activity only at 2nM and 25pM, the magnitude of the response depending also on substrate concentration. The stimulation was blocked by the specific inhibitors methiothepin and ketanserin. We postulate that the organism partially prevents renal mineralocorticoid receptor occupancy by glucocorticoids, circulating at enhanced levels under stressful situations, through serotonin-mediated catabolic regulation of the 11β-hydroxysteroid dehydrogenase 2 activity. Given many, mostly positive, interactions between both hormones, this might eventually pave the way to studies on a new regulatory axis.

Registro:

Documento: Artículo
Título:MDCK cells express serotonin-regulable 11β-hydroxysteroid dehydrogenase type 2
Autor:Zallocchi, M.L.; Damasco, M.C.; Calvo, J.C.; Lantos, C.P.; Matkovic', L.B.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, PRHOM-CONICET, Buenos Aires, Argentina
Instituto de Biología Y Medicina Experimental (CONICET), Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Ciudad Universitaria, (1428) Buenos Aires, Argentina
Palabras clave:11β-HSD2; Glucocorticoid; Mineralocorticoid; Serotonin; Stress hormones; 11beta hydroxysteroid dehydrogenase; 11beta hydroxysteroid dehydrogenase 2; corticosterone; dehydrocorticosterone; glucocorticoid; ketanserin; metitepine; mineralocorticoid receptor; serotonin; unclassified drug; animal cell; article; cell strain; chemical analysis; enzyme activation; enzyme activity; enzyme analysis; enzyme inhibition; enzyme substrate; epithelium cell; high performance liquid chromatography; hormone action; nephron; nonhuman; protein expression; 11-beta-Hydroxysteroid Dehydrogenase Type 2; Animals; Cell Line; Corticosterone; Dogs; Enzyme Activation; Nephrons; Paracrine Communication; Serotonin
Año:2006
Volumen:30
Número:3
Página de inicio:469
Página de fin:477
Título revista:Biocell
Título revista abreviado:Biocell
ISSN:03279545
CODEN:BOCEE
CAS:11beta hydroxysteroid dehydrogenase, 9041-46-7; corticosterone, 50-22-6; dehydrocorticosterone, 72-23-1; ketanserin, 74050-98-9; metitepine, 20229-30-5; serotonin, 50-67-9; 11-beta-Hydroxysteroid Dehydrogenase Type 2, 1.1.1.146; 11-dehydrocorticosterone, 72-23-1; Corticosterone, 50-22-6; Serotonin, 50-67-9
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_03279545_v30_n3_p469_Zallocchi.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v30_n3_p469_Zallocchi

Referencias:

  • Agarwal, A., Expression of HSD11K (NAD+dependent 11β-hydroxysteroid dehydrogenase) promoter constructs in renal cell lines (2000) Endocr Res, 26, pp. 289-302
  • Azmitia, E., Segal, M., An autoradiographic analysis of the differential ascending projections of the dorsal and median raphe nuclei in the rat (1978) J Comp Neurol, 179, pp. 641-667
  • Berndt, T., Liang, T., Tyce, G., Knox, F., Intrarenal serotonin, dopamine, and phosphate handling in remnant kidney (2001) Kidney Int, 59, pp. 625-630
  • Bradford, M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein dye binding (1976) Anal Biochem, 72, pp. 248-254
  • Chaouloff, F., Serotonin, stress and corticoids (2000) J Psychopharmacol, 14, pp. 139-151
  • Contessc, V., Reznik, Y., Louiset, E., Duparc, C., Cartier, D., Sicard, E., Laquerriere, A., Lefebvre, H., Abnormal sensitivity of cortisol-producing adrenocortical adenomas to serotonin: In vivo and in vitro studies (2005) J Clin Endocrinol Metab, , Epub ahead of print
  • De Kloet, E., Sybmesma, H., Reul, H., (1986) Neuroendocrinol, 42, pp. 513-521
  • Devi, L., Heterodimerization of G-protein-coupled receptors: Pharmacology, signalling and trafficking (2001) TRENDS Pharmacol Sci, 22, pp. 532-537
  • Dinan, T., Serotonin and the regulation of hypothalatnic-pituitary- adrenal axis function (1996) Life Sci, 58, pp. 1683-1694
  • Edwards, C., Stewart, P., Burt, D., Brett, L., McIntyre, M., Sutanto, W., de Kloet, E., Monder, C., Localisation of 11β-hydroxysteroid dehydrogenase tissue specific protector of the mineralocorticoid receptor (1988) Lancet, 2, pp. 986-989
  • Farman, N., Bocchi, B., Mineralocorticoid selectivity: Molecular and cellular aspects (2000) Kidney Int, 57, pp. 1364-1369
  • Funder, J., Aldosterone, salt and cardiac fibrosis (1997) Clin Exp Hypertens, 19, pp. 885-899
  • Funder, J., Pearce, P., Smith, R., Smith, A., Mineralocorticoid action: Target tissue specificity is enzyme, not receptor mediated (1988) Science, 242, pp. 583-585
  • Gaush, C., Hard, W., Smith, T., Characterization of an established line of canine kidney cells (MDCK) (1966) Proc Soc Exp Biol Med, 122, pp. 931-935
  • Gekle, M., Freudinger, R., Mildenberger, S., Sauvant, C., Determination of basolateral Na+/H+ exchange activity in MDCK cells using a multiwell-multilabel reader (2001) Anal Biochem, 296, pp. 174-178
  • Gross, J., Berndt, T., Knox, F., Effect of serotonin receptor antagonist on phosphate excretion (2000) J Am Soc Nephrol, 11, pp. 1002-1007
  • Gstraunthaler, G., Pfaller, W., Kotanko, P., Biochemical characterization of renal epithelial cell cultures (LLC-PK1 and MDCK) (1985) Am J Physiol, 248, pp. F536-F544
  • Hesen, W., Joels, M., Modulation of 5HT1A responsiveness in CA1 pyramidal neurons by in vivo activation of corticosteroid receptor (1996) J Neuroendocrinol, 8, pp. 433-438
  • Hoyer, G., Tsiakiras, D., Siebe, H., Hierholzer, K., Corticosteroid metabolism in isolated rat kidney in vitro. III. Structure analysis of lipid soluble metabolites of corticosterone (1984) Pflugers Arch, 400, pp. 377-380
  • Igarreta, P., Calvo, J.C., Damasco, M.C., Activity of renal 11βhydroxysteroid dehydrogenase 2 (11βHSD2) in stressed animals (1999) Life Sci, 64, pp. 2285-2290
  • Jorgensen, H., Knigge, U., Kjaer, A., Warberg, J., Adrenocorticotropic hormone secretion in rats induced by stimulation with serotonergic compounds (1999) J Neuroendocrinol, 11, pp. 283-290
  • Korbmacher, C., Schulz, W., Konig, M., Siebe, H., Lichtenstein, I., Hierholzer, K., Renal epithelial cell lines (BSC-1, MDCK, LLC-PK1) express 11 beta-hydroxysteroid dehydrogenase activity (1989) Biochim Biophys Acta, 1010, pp. 311-317
  • Laplante, P., Diorio, J., Meaney, M., Serotonin regulates hippocampal glucocorticoid receptor expression via a 5-HT7 receptor (2002) Brain Res Dev Brain Res, 139, pp. 199-203
  • Leckie, C., Chapman, K., Edwards, C., Seckl, J., LLC-PK1 cells model 11β-hydroxysteroid dehydrogenase type 2 regulation of glucocorticoid access to renal mineralocorticoid receptors (1995) Endocrinology, 136, pp. 5561-5569
  • Lefebvre, H., Compagnon, P., Contesse, V., Delarue, C., Thuillez, C., Vaudry, H., Kuhn, J., Production and metabolism of serotonin (5-HT) by the human adrenal cortex: Paracrine stimulation of aldosterone secretion by 5-HT (2001) J Clin Endocrinol Metab, 86, pp. 5001-5007
  • McEwen, B., Interacting mediators of allostasis and allostatic load: Towards an understanding of resilience in aging (2003) Metabolism, 52, pp. 10-16
  • McEwen, B., Protective and damaging effects of stress mediators (1998) New Engl J Med, 338, pp. 171-179
  • McKittrick C, McEwen B (1996). Regulation of serotoninergic function in the CNS by steroid hormones and stress In: Stone T, editor: CNS: Neurotransmitters and Neuromodulators. Neuroactive Steroids. New York: CRC Press Inc. pp 37-76; Oberleithner, H., Steigner, W., Silbernagl, S., Vogel, U., Gstraunthaler, G., Pfaller, W., Madin-Darby canine kidney cells. III. Aldosterone stimulates an apical H+/K+ pump (1990) Pflugers Archiv, 416, pp. 540-547
  • Odermatt, A., Arnold, P., Frey, F., The intracellular localization of the mineralocorticoid receptor is regulated by 11β-hydroxysteroid dehydrogenase type 2 (2001) J Biol Chem, 276, pp. 28484-28492
  • Pacak, K., Palkovits, M., Stressor specificity of control neuroendocrine responses: Implication for stress-related disorders (2001) Endocr Rev, 22, pp. 502-548
  • Rusvai, E., Naray-Fejes-Toth, A., A new isoform of 11β-hydroxysteroid dehydrogenase in aldosterone target cells (1993) J Biol Chem, 268, pp. 10717-10720
  • Sanders-Bush, E., Mayer, S., 5-hydroxytriptamine (serotonin): Receptor agonists and antagonists (2001) Goodman & Gilman's The Pharmacological Basis of Therapeutics, pp. 269-290. , Hardman JG, Limbird LE, Gilman AG, editors
  • Sapolsky, R., Romero, L., Munck, A., How do glucocorticoids influence stress responses? Integrating permissive, suppressive, stimulatory, and preparative actions (2000) Endocr Rev, 21, pp. 55-89
  • Schoeffter P, Bobirnac I (1995). 5-hydroxytryptamine 5HT1D receptors mediating inhibition of cyclic AMP accumulation in Madin-Darby canine kidney (MDCK) cells. NaunynSchmiedebergs Arch Pharmacol 352: 256-262; Seckl, J., Expression and properties of corticosteroid receptors in the CNS (1996) CNS: Neurotransmitters and Neuromodulators. Neuroactive Steroids, pp. 1-21. , Stone T, editor:, New York: CRC Press Inc. pp
  • Simmons, N.L., Cultured monolayers of MDCK cells: A novel model system for the study of epithelial development and function (1982) Gen Pharmacol, 13, pp. 287-291
  • Tafet, G., Toister-Achituv, M., Shinitzky, M., Enhancement of serotonin uptake by cortisol: A possible link between stress and depression (2001) Cogn Affect Behav Neurosci, 1, pp. 96-104
  • Tao, Y., Johnson, N., Segaloff, D., Constitutive and agonist-dependent self-association of cell surface human lutropin receptor (2004) J Biol Chem, 279, pp. 5904-5914
  • Wang, T., Berndt, T., Gross, J., Peterson, M., So, M., Knox, F., Effect of inhibition of MAO and COMT on intrarenal dopamine and serotonin and on renal function (2001) Am J Physiol Regul Integr Comp Physiol, 280, pp. R248-R254
  • Xie, Z., Lee, S., O'Dowd, B., George, S., Serotonin 5-HT1B and 5-HT1D receptors form homodimers when expressed alone and heterodimers when co-expressed (1999) FEBS Lett, 456, pp. 63-67
  • Yeager, M.P., Guyre, P.M., Munck, A.U., Glucocorticoid regulation of the inflammatory response to injury (2004) Acta Anaesthesiol Scand, 48 (7), pp. 799-813. , Aug;
  • Young, M., Funder, J., Mineralocorticoid receptors and pathophysiology roles for aldosterone in the cardiovascular system (2002) J Hypertens, 20, pp. 1465-1468
  • Zallocchi, M., Matkovic', L., Calvo, J.C., Damasco, M.C., Adrenal gland involvement in the regulation of renal 11β-hydroxysteroid dehydrogenase 2 (2004) J Cell Biochem, 92, pp. 591-602
  • Zallocchi, M.L., Matkovic', L., Damasco, M.C., Adrenal 11-beta hydroxysteroid dehydrogenase activity in response to stress (2004) Can J Physiol Pharmacol, 82 (6), pp. 422-425. , Jun;
  • Zhou, M., Gomez-Sanchez, E., Cox, D., Cosby, D., Gomez-Sanchez, C., Cloning, expression, and tissue distribution of the rat nicotinamide adenine dinucleotide-dependent 11 beta-hydroxysteroid dehydrogenase (1995) Endocrinology, 136, pp. 3729-3734

Citas:

---------- APA ----------
Zallocchi, M.L., Damasco, M.C., Calvo, J.C., Lantos, C.P. & Matkovic', L.B. (2006) . MDCK cells express serotonin-regulable 11β-hydroxysteroid dehydrogenase type 2. Biocell, 30(3), 469-477.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v30_n3_p469_Zallocchi [ ]
---------- CHICAGO ----------
Zallocchi, M.L., Damasco, M.C., Calvo, J.C., Lantos, C.P., Matkovic', L.B. "MDCK cells express serotonin-regulable 11β-hydroxysteroid dehydrogenase type 2" . Biocell 30, no. 3 (2006) : 469-477.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v30_n3_p469_Zallocchi [ ]
---------- MLA ----------
Zallocchi, M.L., Damasco, M.C., Calvo, J.C., Lantos, C.P., Matkovic', L.B. "MDCK cells express serotonin-regulable 11β-hydroxysteroid dehydrogenase type 2" . Biocell, vol. 30, no. 3, 2006, pp. 469-477.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v30_n3_p469_Zallocchi [ ]
---------- VANCOUVER ----------
Zallocchi, M.L., Damasco, M.C., Calvo, J.C., Lantos, C.P., Matkovic', L.B. MDCK cells express serotonin-regulable 11β-hydroxysteroid dehydrogenase type 2. Biocell. 2006;30(3):469-477.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v30_n3_p469_Zallocchi [ ]