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Abstract:

Endothelial cells, at the cell-cell borders, express PECAM-1, and have been implicated in vascular functions. The monoclonal antibody MEC 13.3 recognizes PECAM-1 molecule from mouse vessels and allows to analyze the ontogeny of mouse endothelium. At the present, little is known about the molecular basis of differentiation pathways of endothelial cells, that enables its morphological heterogeneity. The purpose of this study was to analyze the pattern of PECAM-1 expression, employing monoclonal antibody MEC 13.3, in cellular suspensions obtained from different mouse organs at pre and postnatal stages. Fluorescence activated cell sorter analysis showed a different profile of the glycoprotein expression in a cell population with size and granularity selected by 1G11 endothelial cell line. The expression differs from prenatal to postnatal developmental stages in a given organ, and among the organs studied. Another cell population, with a size and granularity higher than 1G11 endothelial cell line, coexists in cellular suspensions obtained from liver, gut and brain. These cells could be related to those detected by means of immunoenzyme methods which showed a non-differentiated morphology. The different PECAM-1 pattern expression could reflect potential organ-specific differentiation pathways during development and according to organs environment. The existence of another cell population with a size and granularity higher than 1G11 endothelial cell line required a phenotypic characterization.

Registro:

Documento: Artículo
Título:Tissue expression of platelet endothelial cell adhesion molecule-1 at pre and postnatal murine development
Autor:Calabrese, G.C.; Wainstok, R.
Filiación:Cat. de Biol. Celular e Histologia, Departamento de Ciencias Biologicas, Universidad de Buenos Aires, Junin 954/6, 1113 Buenos Aires, Argentina
Departamento de Quimica Biologica, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Nuñez, 1428 Buenos Aires, Argentina
Palabras clave:Endothelial cells; Mouse organs; PECAM-1; Vasculogenesis; CD31 antigen; glycoprotein; monoclonal antibody; monoclonal antibody mec 13.3; unclassified drug; animal cell; antibody detection; article; blood vessel function; brain; cell adhesion; cell differentiation; cell heterogeneity; cell population; cell suspension; embryo; endothelium cell; enzyme immunoassay; fluorescence activated cell sorting; intestine; liver; molecular biology; morphological adaptation; mouse; newborn; nonhuman; ontogeny; phenotype; postnatal development; prenatal development; protein expression; Animals; Antigens, CD31; Brain; Brain Chemistry; Cell Adhesion Molecules; Cell Differentiation; Cells, Cultured; Embryo; Endothelial Cells; Flow Cytometry; Immunohistochemistry; Intestines; Liver; Mice; Mice, Inbred BALB C; Time Factors; Animalia; Murinae
Año:2004
Volumen:28
Número:3
Página de inicio:251
Página de fin:258
Handle:http://hdl.handle.net/20.500.12110/paper_03279545_v28_n3_p251_Calabrese
Título revista:Biocell
Título revista abreviado:Biocell
ISSN:03279545
CODEN:BOCEE
CAS:Antigens, CD31; Cell Adhesion Molecules
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_03279545_v28_n3_p251_Calabrese.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v28_n3_p251_Calabrese

Referencias:

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Citas:

---------- APA ----------
Calabrese, G.C. & Wainstok, R. (2004) . Tissue expression of platelet endothelial cell adhesion molecule-1 at pre and postnatal murine development. Biocell, 28(3), 251-258.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v28_n3_p251_Calabrese [ ]
---------- CHICAGO ----------
Calabrese, G.C., Wainstok, R. "Tissue expression of platelet endothelial cell adhesion molecule-1 at pre and postnatal murine development" . Biocell 28, no. 3 (2004) : 251-258.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v28_n3_p251_Calabrese [ ]
---------- MLA ----------
Calabrese, G.C., Wainstok, R. "Tissue expression of platelet endothelial cell adhesion molecule-1 at pre and postnatal murine development" . Biocell, vol. 28, no. 3, 2004, pp. 251-258.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v28_n3_p251_Calabrese [ ]
---------- VANCOUVER ----------
Calabrese, G.C., Wainstok, R. Tissue expression of platelet endothelial cell adhesion molecule-1 at pre and postnatal murine development. Biocell. 2004;28(3):251-258.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v28_n3_p251_Calabrese [ ]