Artículo
Goldstein, J.; Paveto, C.; López-Costa, J.J.; Pereira, C.; Alonso, G.; Torres, H.N.; Flawia, M.M. "Immuno and cytochemical localization of Trypanosoma cruzi nitric oxide synthase" (2000) Biocell. 24(3):217-222
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Abstract:
The localization and subcellular distribution of Trypanosoma cruzi nitric oxide synthase was investigated in epimastigote cells by immunocytochemistry at electron and light microscope level, using a polyclonal antibody to neuronal nitric oxide synthase, and also, at light microscope level, by the nicotinamide adenine dinucleotide phosphate-diaphorase histochemical reaction. The immunoreactivity was ultrastructurally localized by electron microscopy in the inner surface of cell membranes and in free cytosolic clusters in the body, flagellum and apical extreme. Light microscopy showed that immunoprecipitates, specific for the Trypanosoma cruzi nitric oxide synthase, co-localized with the formazan precipitates generated by the diaphorase reaction in the same areas identified by electron microscopy. These results, taken together with previous finding from our laboratory could help to explain the involvement of the nitric oxide transduction pathway in T. cruzi epimastigote motility.
Registro:
| Documento: | Artículo |
| Título: | Immuno and cytochemical localization of Trypanosoma cruzi nitric oxide synthase |
| Autor: | Goldstein, J.; Paveto, C.; López-Costa, J.J.; Pereira, C.; Alonso, G.; Torres, H.N.; Flawia, M.M. |
| Filiación: | Inst. Invest. en Ingenieria Genet., Biologia Molecular, Fac. de Ciencias Exactas/Naturales, (1428) Buenos Aires, Argentina |
| Palabras clave: | Diaphorase; Immunocytochemistry; Nitric oxide synthase; Subcellular localization; Trypanosoma cruzi; formazan; nitric oxide synthase; reduced nicotinamide adenine dinucleotide phosphate dehydrogenase; animal cell; apical membrane; article; cell membrane; cell motility; cell ultrastructure; cellular distribution; enzyme localization; epimastigote; flagellum; immunocytochemistry; nonhuman; parasite migration; signal transduction; Trypanosoma cruzi; Animals; Cell Compartmentation; Cell Membrane; Cell Movement; Flagella; Nitric Oxide; Nitric Oxide Synthase; Signal Transduction; Trypanosoma cruzi; Animalia; Trypanosoma; Trypanosoma cruzi |
| Año: | 2000 |
| Volumen: | 24 |
| Número: | 3 |
| Página de inicio: | 217 |
| Página de fin: | 222 |
| Título revista: | Biocell |
| Título revista abreviado: | Biocell |
| ISSN: | 03279545 |
| CODEN: | BOCEE |
| CAS: | Nitric Oxide Synthase, EC 1.14.13.39; Nitric Oxide, 10102-43-9 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v24_n3_p217_Goldstein |
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Citas:
---------- APA ----------
Goldstein, J., Paveto, C., López-Costa, J.J., Pereira, C., Alonso, G., Torres, H.N. & Flawia, M.M. (2000) . Immuno and cytochemical localization of Trypanosoma cruzi nitric oxide synthase. Biocell, 24(3), 217-222.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v24_n3_p217_Goldstein [ ]
---------- CHICAGO ----------
Goldstein, J., Paveto, C., López-Costa, J.J., Pereira, C., Alonso, G., Torres, H.N., et al. "Immuno and cytochemical localization of Trypanosoma cruzi nitric oxide synthase" . Biocell 24, no. 3 (2000) : 217-222.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v24_n3_p217_Goldstein [ ]
---------- MLA ----------
Goldstein, J., Paveto, C., López-Costa, J.J., Pereira, C., Alonso, G., Torres, H.N., et al. "Immuno and cytochemical localization of Trypanosoma cruzi nitric oxide synthase" . Biocell, vol. 24, no. 3, 2000, pp. 217-222.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v24_n3_p217_Goldstein [ ]
---------- VANCOUVER ----------
Goldstein, J., Paveto, C., López-Costa, J.J., Pereira, C., Alonso, G., Torres, H.N., et al. Immuno and cytochemical localization of Trypanosoma cruzi nitric oxide synthase. Biocell. 2000;24(3):217-222.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v24_n3_p217_Goldstein [ ]
