Artículo
Llanos, S.L.; Roldán, A. "Effect of dexamethasone on nitric oxide (NO·) Production by cultured astrocytes" (1999) Biocell. 23(1):29-35
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Abstract:
Glial cell-derived nitric oxide (NO·) and its regulation has significant implications for central nervous system pathophysiology. The aim of the present study was to determine the production of NO· by cultured rat astrocytes and peritoneal macrophages, and its regulation by glucocorticoids. Results corroborate the presence of both constitutive and induced NO· synthase (cNOS and iNOS) in astrocytes, while macrophages express only the inducible isoform. Dexamethasone was able to decrease NO· synthesis produced by induction of iNOS in macrophage and astrocyte phagocytosis, but not the NO· produced by the cNOS spontaneously expressed in cultured astrocytes.
Registro:
| Documento: | Artículo |
| Título: | Effect of dexamethasone on nitric oxide (NO·) Production by cultured astrocytes |
| Autor: | Llanos, S.L.; Roldán, A. |
| Filiación: | Inst. de Biol. y Med. Experimental, Vuelta de Obligado 2490, Buenos Aires 1428, Argentina |
| Palabras clave: | Astrocytes; Dexamethasone; Macrophages; NADPH-diaphorase; NO·; Phagocytosis; dexamethasone; glucocorticoid; isoenzyme; nitric oxide; nitric oxide synthase; reduced nicotinamide adenine dinucleotide phosphate dehydrogenase; zymosan; animal cell; article; astrocyte; controlled study; enzyme activation; enzyme activity; hormonal regulation; newborn; nonhuman; peritoneum macrophage; phagocytosis; protein expression; rat; Animals; Animals, Newborn; Astrocytes; Cells, Cultured; Cerebral Cortex; Dexamethasone; Glucocorticoids; Nitric Oxide; Rats; Rats, Sprague-Dawley |
| Año: | 1999 |
| Volumen: | 23 |
| Número: | 1 |
| Página de inicio: | 29 |
| Página de fin: | 35 |
| Título revista: | Biocell |
| Título revista abreviado: | Biocell |
| ISSN: | 03279545 |
| CODEN: | BOCEE |
| CAS: | Dexamethasone, 50-02-2; Glucocorticoids; Nitric Oxide, 10102-43-9 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v23_n1_p29_Llanos |
Referencias:
- Baltrons, M.A., Agulló, L., García, A., Dexamethasone up-regulates constitutive nitric oxide synthase in cerebellar astrocytes but not in granule cells in culture (1995) J Neurochemistry, 64, pp. 447-450
- Beckman, J.S., Peroxynitrite versus hydroxyl radicals: The role of nitric oxide in superoxide-dependent cerebral injury (1994) Annals N.Y. Acad. Science, 738, pp. 69-75
- Chao, C.C., Hu, S., Sheng, W.S., Bu, D., Bukrinsky, M.I., Peterson, P.K., Cytokine-stimulated astrocytes damage human neurons via a nitric oxide mechanism (1996) Glia, 16, pp. 276-284
- Colton, C.A., Gilbert, D.L., Microglia an in vivo source of reactive oxygen species in the brain (1993) Advances in Neurology, 59, pp. 321-3226
- Colton, C.A., Snell, J., Chernyshev, O., Gilbert, D., Induction of superoxide anion and nitric oxide production in culture (1994) Microglia Annals N.Y. Acad. Science, 738, pp. 54-63
- Dawson, T.M., Dawson, V.L., Snyder, S.H., A novel neuronal messenger molecule in brain: The free radical, nitric oxide (1992) Ann Neurol, 32, pp. 297-311
- Di Rosa, M., Radomski, M., Carnuccio, R., Moncada, S., Glucocorticoids inhibit the induction of nitric oxide synthase in macrophages (1990) Bioch Bhiophys Res Comm, 172, pp. 1246-1252
- Galea, E., Feinstein, D.L., Reis, D.J., Induction of calcium-independent nitric oxide synthase activity in primary rat glial cultures (1992) Proc Nact Acad Sciences, 89, pp. 10945-10949
- Green, L.C., Wagner, D.A., Glouwgovski, Y., Skepper, P.L., Wishnok, J.S., Tennebaum, S.R., Analysis of nitrate biological fluids (1982) Anal Biochem, 124, pp. 131-138
- Greenberg, S.J., Khoury, J.E., Kaplan, E., Silverstein, S.C., A fluorescent technique to distinguish attached from ingested erythrocytes and zymosan particles in phagocytosing macrophages (1991) Immunol Methods, 139, pp. 115-122
- Kunz, D., Walker, G., Eberhardt, W., Pfeilschifter, J., Molecular mechanism of dexamethasone inhibition of nitric oxide synthase expression in interleukin 1β-stimulated mesangial cells: Evidence for the involvement of transcriptional and post-transcriptional regulation (1996) Proc Natl Acad Sci USA, 93, pp. 255-259
- Murphy, S., Simmons, M.L., Agulló, L., García, A., Feinstein, D.L., Galea, E., Reis, D.J., Schawrtz, J.P., Synthesis of nitric oxide in CNS glial cells (1993) Trends in Neurosciences, 16, pp. 323-328
- Roldán, A., Gogg, S., Ferrini, M., Schillaci, R., De Nicola, A.F., Glucocorticoid regulation of in vitro astrocyte phagocytosis (1997) Biocell, 21, pp. 93-99
- Stamler, J.S., Singel, D.J., Loscalzo, J., Biochemistry of nitric oxide and its redox-activated forms (1992) Science, 258, pp. 1898-1902
Citas:
---------- APA ----------
Llanos, S.L. & Roldán, A. (1999) . Effect of dexamethasone on nitric oxide (NO·) Production by cultured astrocytes. Biocell, 23(1), 29-35.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v23_n1_p29_Llanos [ ]
---------- CHICAGO ----------
Llanos, S.L., Roldán, A. "Effect of dexamethasone on nitric oxide (NO·) Production by cultured astrocytes" . Biocell 23, no. 1 (1999) : 29-35.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v23_n1_p29_Llanos [ ]
---------- MLA ----------
Llanos, S.L., Roldán, A. "Effect of dexamethasone on nitric oxide (NO·) Production by cultured astrocytes" . Biocell, vol. 23, no. 1, 1999, pp. 29-35.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v23_n1_p29_Llanos [ ]
---------- VANCOUVER ----------
Llanos, S.L., Roldán, A. Effect of dexamethasone on nitric oxide (NO·) Production by cultured astrocytes. Biocell. 1999;23(1):29-35.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v23_n1_p29_Llanos [ ]
