Abstract:
A simple photographic method for histological sections is described. This is a contact method which does not require the use of photographic camera. It is very similar to the contact print method used routinely in photographic laboratories. It is performed by placing the slides with the histological section; coverslip downwards, over the emulsion of the negative film. Then, they are illuminated with a common photographic enlarger during a brief period of time. Thus, a negative contact print of the section is obtained. This technique yields photographs of high quality focus, sharply contrasted with great detail. These results may also be obtained with low- contrast stained sections. This method is most suitable for photography of large or medium-sized histological sections (macros). This is often the case of neuroanatomical and immunohistochemical studies. Our method, being simple and quick, is recommended for routine use in laboratories.
Registro:
Documento: |
Artículo
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Título: | A simple method for taking photographs of histological sections without using neither photographic camera nor microscope |
Autor: | Carmanchahi, P.D.; Aldana Marcos, H.J.; Ferrari, C.C.; Affanni, J.M. |
Filiación: | Instituto de Neurociencia, Fac. de Ciencias Exactas y Naturales, Ciudad Universitaria, (1428) Buenos Aires, Argentina
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Palabras clave: | Histology; Immunohistochemistry; Neuroanatomy; Photography; Retrograde neuronal tracing; article; emulsion; film; histology; human; illumination; immunohistochemistry; neuroanatomy; nonhuman; photography; Histological Techniques; Photomicrography |
Año: | 1998
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Volumen: | 22
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Número: | 3
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Página de inicio: | 207
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Página de fin: | 210
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Título revista: | Biocell
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Título revista abreviado: | Biocell
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ISSN: | 03279545
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CODEN: | BOCEE
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v22_n3_p207_Carmanchahi |
Referencias:
- Aldana Marcos, H.J., Ferrari, C.C., Benitez, I., Affanni, J.M., Standarization of fixation, processing and staining method for the central nervous system of vertebrates (1996) Biocell, 20 (3), pp. 265-272
- Briscoe, M.E., (1996) Preparing Scientific Illustrations. A Guide to Better Posters, Presentations and Publications, pp. 1-9. , Springer-Verlag, New York
- Johannessen, J.V., Finseth, I., Pergins, K.H., Sobrinho-Simoss, M., A new approach to low power photomicrography (1981) J Clin Path, 34, pp. 564-567
- Mesulam, M.M., Tetramethylbenzidine for horseradish peroxidase neurohistochemistry: A non-carcinogenic blue reaction-product with superior sensitivity for visualizing neural afferents and efferents (1978) J Histochem Cytochem, 26, pp. 106-117
- Oswaldo-Cruz, E., Rocha Miranda, C.E., (1968) The Brain of the Opossum (Didelphis Marsupialis), p. 5. , Instituto de Biofísica. Universidade Federal do Rio de Janeiro. Brasil
- Ramon, Y., Cajal, S., Continuación de los reactivos (1901) Elementos de Histología Normal y de Técnica Micrográfica, pp. 84-96. , Moya S., Madrid
- Shanthaveerappa, T.R., Manocha, S.L., Bourne, G.H., Macrophotography of thick sections and gels with a regular photographic enlarger (1965) Stain Technol, 40, p. 309
Citas:
---------- APA ----------
Carmanchahi, P.D., Aldana Marcos, H.J., Ferrari, C.C. & Affanni, J.M.
(1998)
. A simple method for taking photographs of histological sections without using neither photographic camera nor microscope. Biocell, 22(3), 207-210.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v22_n3_p207_Carmanchahi [ ]
---------- CHICAGO ----------
Carmanchahi, P.D., Aldana Marcos, H.J., Ferrari, C.C., Affanni, J.M.
"A simple method for taking photographs of histological sections without using neither photographic camera nor microscope"
. Biocell 22, no. 3
(1998) : 207-210.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v22_n3_p207_Carmanchahi [ ]
---------- MLA ----------
Carmanchahi, P.D., Aldana Marcos, H.J., Ferrari, C.C., Affanni, J.M.
"A simple method for taking photographs of histological sections without using neither photographic camera nor microscope"
. Biocell, vol. 22, no. 3, 1998, pp. 207-210.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v22_n3_p207_Carmanchahi [ ]
---------- VANCOUVER ----------
Carmanchahi, P.D., Aldana Marcos, H.J., Ferrari, C.C., Affanni, J.M. A simple method for taking photographs of histological sections without using neither photographic camera nor microscope. Biocell. 1998;22(3):207-210.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v22_n3_p207_Carmanchahi [ ]