Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte la política de Acceso Abierto del editor

Abstract:

Astrocytes participate in central nervous system injury, degenerative diseases and also perform macrophagic functions. The present work investigates: 1) the effect of the physiological glucocorticoid corticosterone (CORT) and the synthetic agonist dexamethasone (DEX) on latex beads phagocytosis by neonatal rat cortical astrocytes in culture, and 2) the expression of immunoreactive glucocorticoid receptors (GR) in astrocytes cultured in different media with or without a pulse application of CORT. The results indicated that glucocorticoids reduced astrocyte phagocytic activity, as occurred with macrophages, independently of the culturing conditions employed. The extent of phagocytosis was inversely related to nuclear immunostaining for GR in cultures in fetal calf serum, which contained endogenous glucocorticoid. However, no correlation was found between nuclear GR and phagocytosis for cultures in glucocorticoid-free medium or in medium containing CORT. It is suggested that additional factors, besides the GR, may be involved in glucocorticoid modulation of astrocyte phagocytosis.

Registro:

Documento: Artículo
Título:Glucocorticoid regulation of in vitro astrocyte phagocytosis
Autor:Roldán, A.; Gogg, S.; Ferrini, M.; Schillaci, R.; De Nicola, A.F.
Filiación:Inst. de Biol. y Med. Experimental, Vta. de Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:Astrocytes phagocytosis; Corticosterone; Dexamethasone; Glucocorticoid receptors; antiinflammatory agent; corticosterone; dexamethasone; glucocorticoid; glucocorticoid receptor; animal; article; astrocyte; brain cortex; cell culture; chemistry; cytology; drug effect; immunology; metabolism; phagocytosis; physiology; rat; Sprague Dawley rat; Animals; Anti-Inflammatory Agents; Astrocytes; Cells, Cultured; Cerebral Cortex; Corticosterone; Dexamethasone; Glucocorticoids; Phagocytosis; Rats; Rats, Sprague-Dawley; Receptors, Glucocorticoid
Año:1997
Volumen:21
Número:1
Página de inicio:83
Página de fin:89
Título revista:Biocell
Título revista abreviado:Biocell
ISSN:03279545
CODEN:BOCEE
CAS:Anti-Inflammatory Agents; Corticosterone, 50-22-6; Dexamethasone, 50-02-2; Glucocorticoids; Receptors, Glucocorticoid
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v21_n1_p83_Roldan

Referencias:

  • Ahima, R.S., Garcia, M.M., Harlan, R.E., Intracellular localization of corticosteroid receptorsin brain: Potential interactions with signal transduction pathways (1992) Proc Soc Exp Biol Med, 201, pp. 244-253
  • Becker, J., Grasso, R.J., Suppression of phagocytosis by dexamethasone in macrophage cultures: Inability of arachidonic acid, indomethacin and nordihydroguaiaretic acid to reverse the inhibitory response mediated by a steroid-inducible factor (1985) Int J Immunopharmac, 7, pp. 839-847
  • Cronstein, B.N., Kimmel, S.C., Levin, R.I., Martiniuk, F., A mechanism for the anti-inflammatory effects of corticosteroids: The glucocorticoid receptor regulates leukocyte adhesion to endothelial cells and expression of endothelial-leukocyte adhesion molecule 1 and intercellular adhesion molecule 1 (1992) Proc Natl Acad Sci, 89, pp. 9991-9995
  • De Kloet, E.R., Oitzl, M.S., Joels, M., Functional implications of brain corticosteroid receptor diversity (1993) Cell Mol Neurobiol, 13, pp. 433-455
  • Dubrovsky, B., Williams, D., Kraulis, I., Effects of corticosterone and 5α-dihydrocorticosterone on brain excitability in the rat (1985) J Neurosci Res, 14, pp. 117-128
  • Eng, L.F., Glial fibrillary acidic protein (GFAP): The major protein of glial intermediate filaments I differentiated astrocytes (1985) J Neuroimmunol, 8, pp. 203-214
  • Fauci, A.S., Immunosuppressive and anti-inflammatory effects of glucocorticoids (1979) Glucocorticoid Hormone Action, pp. 449-465. , J.D. Baxter and G.G. Rousseau Eds. Springer-Verlag, New York
  • Ferrini, M., Lima, A., De Nicola, A.F., Estradiol abolishes autologous down-regulation of glucocorticoid receptors in brain (1995) Life Sci, 26, pp. 2403-2412
  • Fuxe, K., Wikstrom, A.-C., Okret, S., Agnati, L.F., Harfstrand, A., Yuan, Z.-Y., Granholm, L., Gustaffson, J.-A., Mapping of immunoreactive neurons in the rat tel- and diencephalon using a monoclonal antibody againts rat liver glucocorticoid receptor (1985) Endocrinology, 117, pp. 1803-1812
  • Gebicke-Haerter, P., Schobert, A., Dieter, P., Hoegger, P., Hertting, G., Regulation glucocor-ticoid-independent induction of lipocortin 1 in cultured astrocytes (1991) J Neurochem, 57, pp. 175-183
  • Gonzalez, S., Ferrini, M., Coirini, H., Gonzalez Deniselle, M.C., De Nicola, A.F., Regulation of flunitrazepam binding in the dorsal horn of the spinal cord by adrenalectomy and corticosteroids (1992) Brain Res, 589, pp. 97-101
  • Gonzalez Deniselle, M.C., Gonzalez, S., Piroli, G., Lima, A.E., De Nicola, A.F., The 21-aminosteroid U-74389F increases the number of glial fibrillary acidic protein-expressing astrocytes in the spinal cord of control and Wobbler mice (1996) Cell Mol Neurobiol, 16, pp. 61-72
  • Hall, E.D., The neuroprotective pharmacology of methylprednisolone (1992) J Neurosurg, 76, pp. 13-22
  • Hertz, L., McFarlin, D.E., Waksman, B.H., Astrocytes: Auxiliary cells for the immune responses in the central nervous system (1990) Immunol Today, 11, pp. 265-268
  • Iacono, R.F., Berria, M.I., Lascano, E.F., A triple staining procedure to evaluate phagocytic role of differentiated astrocytes (1991) J Neurosci Meth, 39, pp. 225-230
  • Kaur, C., Wu, C.H., Wen, C., Ling, E.A., The effects of subcutaneous injections of glucocorticoids on amoeboid microglia in posnatal rats (1994) Arch Hist Cytol, 57, pp. 449-459
  • Laping, N.J., Teter, B., Nichols, N.R., Rozovsky, I., Finch, C.E., Glial fibrillary acidic protein: Regulation by hormones, cytokines and growth factors (1994) Brain Pathol, 1, pp. 259-275
  • Maccarthy, K.D., De Vellis, J., Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue (1980) J Cell Biol, 85, pp. 890-902
  • McEwen, B.S., Coirini, H., Schumacher, M., Steroid effects on neuronal activity: When is the genome involved? (1990) Ciba Foundation Symposia on Steroids and Neuronal Activity, pp. 3-11. , D. Chadwik K. Widdows, Eds. J. Wiley & Sons, West Sussex, U.K
  • McEwen, B.S., Cameron, H., Chao, H., Gould, E., Magarinos, A.M., Watanabe, Y., Wooley, C., Adrenal steroids and plasticity of hippocampal neurons: Towards an understanding of underlying cellular and molecular mechanisms (1993) Cell Mol Neurobiol, 13, pp. 457-482
  • Merril, J.E., Jonakait, G.M., Interactions of the nervous and immune system during development, normal brain homeostasis and disease (1995) FASEB J, 9, pp. 611-618
  • Munck, A., Guyre, P.M., Holbrook, N.J., Physiological functions of glucocorticoids in stress and their relations to pharmacological actions (1984) Endocrine Rev, 5, pp. 25-44
  • Munck, A., Naray-Fejes-Toth, A., Glucocorticoids and stress: Permissive and suppresive actions (1994) Ann NY Acad Sci, 746, pp. 115-130
  • Noske, W., Lentzen, H., Lange, K., Keller, K., Phagocytic activity of glial cells in culture (1982) Exp Cell Res, 142, pp. 437-445
  • Orchinik, M., Murray, T.F., Moore, F.L., A corticosteroid receptor in neuronal membranes (1991) Science, 252, pp. 1848-1851
  • Patel, A.J., Hunt, A., Faraji-Shadan, F., Effect of removal of glutamine and addition of dexamethasone on the activities of glutamine synthetase, ornithine decarboxylase and lactate dhydrogenase in primary cultures of forebrain and cerebellar astrocytes (1986) Develop Brain Res, 26, pp. 229-238
  • Schaffner, A., Therapeutic concentrations of glucocorticoids suppress antimicrobial activity of human macrophages without imparing their responsiveness to gamma interferon (1985) J Clin Invest, 76, pp. 1755-1760
  • Sharif, S.F., Hairiri, R.J., Chang, V.A., Barie, P.S., Wang, R.S., Ghajar, J.B.G., Human astrocyte production of tumour necrosis factor-α, interleukin-1β, and interleukin-6 following exposure to lipopolysaccharide endotoxin (1993) Neurol Res, 15, pp. 109-112
  • Sutanto, W., Handelmann, G., De Bree, F., De Kloet, E.R., Multifaceted interaction of corticosteroids with the intracellular receptors and with membrane GABAa receptor complex in the rat brain (1989) J Neuroendocrinol, 1, pp. 243-247
  • Tombaugh, G.C., Sapolsky, R.M., Endocrine features of glucocorticoid endangerment in hippocampal astrocytes (1993) Neuroendocrinology, 57, pp. 7-13
  • Wyllie, A.H., Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation (1980) Nature, 284, pp. 555-556

Citas:

---------- APA ----------
Roldán, A., Gogg, S., Ferrini, M., Schillaci, R. & De Nicola, A.F. (1997) . Glucocorticoid regulation of in vitro astrocyte phagocytosis. Biocell, 21(1), 83-89.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v21_n1_p83_Roldan [ ]
---------- CHICAGO ----------
Roldán, A., Gogg, S., Ferrini, M., Schillaci, R., De Nicola, A.F. "Glucocorticoid regulation of in vitro astrocyte phagocytosis" . Biocell 21, no. 1 (1997) : 83-89.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v21_n1_p83_Roldan [ ]
---------- MLA ----------
Roldán, A., Gogg, S., Ferrini, M., Schillaci, R., De Nicola, A.F. "Glucocorticoid regulation of in vitro astrocyte phagocytosis" . Biocell, vol. 21, no. 1, 1997, pp. 83-89.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v21_n1_p83_Roldan [ ]
---------- VANCOUVER ----------
Roldán, A., Gogg, S., Ferrini, M., Schillaci, R., De Nicola, A.F. Glucocorticoid regulation of in vitro astrocyte phagocytosis. Biocell. 1997;21(1):83-89.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v21_n1_p83_Roldan [ ]