Artículo

Vanzulli, S.; Gazzaniga, S.; Braidot, M.F.; Vecchi, A.; Mantovani, A.; Wainstok De Calmanovici, R. "Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors" (1997) Biocell. 21(1):39-46
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Abstract:

In order to clearly visualize blood vessels, the monoclonal antibody (mAb) MEC 13.3 was used for an immunohistochemical staining on frozen sections of different mice mammary tumors. MEC 13.3 mAb is specific for endothelial cells (ECs) of mouse blood vessels and recognizes a molecule related to the murine form of CD31/PECAM. This mAb with immunoenzymatic technique or inmunofluorescent labelling, was found to be a useful tool to quantify tumor neovascularization. Specifically, membrane reinforcement could be observed in vessel ECs, indicating the expression of CD31/ PECAM in their surface. The staining of ECs from tumors and from normal tissues was also compared. In this work, the use of MEC13.3 mAb is reported to recognize mice mammary tumor ECs as a useful tool to identify neovascularization. It would also be helpful for research on the origin and function of vascular endothelium in murine tumor experimental models.

Registro:

Documento: Artículo
Título:Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors
Autor:Vanzulli, S.; Gazzaniga, S.; Braidot, M.F.; Vecchi, A.; Mantovani, A.; Wainstok De Calmanovici, R.
Filiación:Ctro. de Estud. Oncológicos, Academia Nacional de Medicina, Pacheco de Melo 3081, 1425 Buenos Aires, Argentina
Depto. de Quim. Biológica, Fac. de Ciencias Exactas y Naturales, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Ist. Ric. Farmacologiche Mario Negri, Via Eritrea 62, 20157, Milan, Italy
Palabras clave:Endothelial cells; MEC 13.3; Mice mammary tumors; Mouse CD31/PECAM; monoclonal antibody; adenocarcinoma; animal; antibody specificity; antigen antibody reaction; article; Bagg albino mouse; cell culture; chemistry; cytology; experimental neoplasm; female; immunohistochemistry; immunology; microcirculation; mouse; udder; vascular endothelium; vascularization; Adenocarcinoma; Animals; Antibodies, Monoclonal; Antibody Specificity; Antigen-Antibody Reactions; Endothelium, Vascular; Female; Immunohistochemistry; Mammary Glands, Animal; Mammary Neoplasms, Experimental; Mice; Mice, Inbred BALB C; Microcirculation; Tumor Cells, Cultured
Año:1997
Volumen:21
Número:1
Página de inicio:39
Página de fin:46
Título revista:Biocell
Título revista abreviado:Biocell
ISSN:03279545
CODEN:BOCEE
CAS:Antibodies, Monoclonal
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v21_n1_p39_Vanzulli

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Citas:

---------- APA ----------
Vanzulli, S., Gazzaniga, S., Braidot, M.F., Vecchi, A., Mantovani, A. & Wainstok De Calmanovici, R. (1997) . Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors. Biocell, 21(1), 39-46.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v21_n1_p39_Vanzulli [ ]
---------- CHICAGO ----------
Vanzulli, S., Gazzaniga, S., Braidot, M.F., Vecchi, A., Mantovani, A., Wainstok De Calmanovici, R. "Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors" . Biocell 21, no. 1 (1997) : 39-46.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v21_n1_p39_Vanzulli [ ]
---------- MLA ----------
Vanzulli, S., Gazzaniga, S., Braidot, M.F., Vecchi, A., Mantovani, A., Wainstok De Calmanovici, R. "Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors" . Biocell, vol. 21, no. 1, 1997, pp. 39-46.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v21_n1_p39_Vanzulli [ ]
---------- VANCOUVER ----------
Vanzulli, S., Gazzaniga, S., Braidot, M.F., Vecchi, A., Mantovani, A., Wainstok De Calmanovici, R. Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors. Biocell. 1997;21(1):39-46.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v21_n1_p39_Vanzulli [ ]