Artículo
Aldana Marcos, H.J.; Ferrari, C.C.; Benitez, I.; Affanni, J.M. "Standardization of fixation, processing and staining methods for the central nervous system of vertebrates" (1996) Biocell. 20(3):265-272
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Abstract:
This paper reports the standardization of methods used for processing and embedding various vertebrate brains of different sizes in paraffin. Other technical details developed for avoiding frequent difficulties arising during laboratory routine are also reported. Some modifications of the Nissl and Klüver-Barrera staining methods are proposed. These modifications include: 1) a Nissl stain solution with a rapid and efficient action with easier differentiation; 2) the use of a cheap microwave oven for the Klüver-Barrera stain. These procedures have the advantage of permitting Nissl and Klüver-Barrera staining of nervous tissue in about five and fifteen minutes respectively. The proposed procedures have been tested in brains obtained from fish, amphibians, reptiles and mammals of different body sizes. They are the result of our long experience in preparing slides for comparative studies. Serial sections of excellent quality were regularly obtained in all the specimens studied. These standardized methods, being simple and quick, are recommended for routine use in neurobiological laboratories.
Registro:
| Documento: | Artículo |
| Título: | Standardization of fixation, processing and staining methods for the central nervous system of vertebrates |
| Autor: | Aldana Marcos, H.J.; Ferrari, C.C.; Benitez, I.; Affanni, J.M. |
| Filiación: | Instituto de Neurociencia, Fac. de Ciencias Exactas y Naturales, Pab. II, (1428) Buenos Aires, Argentina |
| Palabras clave: | Klüver-Barrera; Microwave oven; Neuroanatomy; Nissl; Paraffin sections; coloring agent; animal; article; central nervous system; histology; laboratory diagnosis; methodology; microtomy; staining; standard; tissue fixation; vertebrate; Animals; Central Nervous System; Coloring Agents; Microtomy; Specimen Handling; Staining and Labeling; Tissue Embedding; Tissue Fixation; Vertebrates |
| Año: | 1996 |
| Volumen: | 20 |
| Número: | 3 |
| Página de inicio: | 265 |
| Página de fin: | 272 |
| Título revista: | Biocell |
| Título revista abreviado: | Biocell |
| ISSN: | 03279545 |
| CODEN: | BOCEE |
| CAS: | Coloring Agents |
| PDF: | https://bibliotecadigital.exactas.uba.ar/download/paper/paper_03279545_v20_n3_p265_AldanaMarcos.pdf |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v20_n3_p265_AldanaMarcos |
Referencias:
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- Gordon, K.C., Tissue processing (1992) Theory and Practice of Histological Techniques, pp. 43-59. , J. Bancroft and A. Stevens, Eds. Churchill Livingstone, London
- Klüver, H., Barrera, E., A method for the combined staining of cells and fibers in the nervous system (1953) J Neuropath Exp Neurol, 12, pp. 400-403
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- Pearse, A.G.E., Lipids, lipoproteins and proteolipids (1985) Histochemistry. Theoretical and Applied, pp. 768-849. , Churchill Livingstone. New York
- Ramon Y Cajal, S., De Castro, F., Procederes generales de disociación, métodos de inclusion en celoidina, parafina y gelatina. Inclusiones superficiales (1972) Elementos de Técnica Micrográfica del Sistema Nervioso, pp. 23-31. , Salvat, Barcelona
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- Windle, W.F., Rhines, R., Rankin, J., A Nissl method using buffered solutions of thionin (1943) Stain Techn, 18, pp. 77-86
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Citas:
---------- APA ----------
Aldana Marcos, H.J., Ferrari, C.C., Benitez, I. & Affanni, J.M. (1996) . Standardization of fixation, processing and staining methods for the central nervous system of vertebrates. Biocell, 20(3), 265-272.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v20_n3_p265_AldanaMarcos [ ]
---------- CHICAGO ----------
Aldana Marcos, H.J., Ferrari, C.C., Benitez, I., Affanni, J.M. "Standardization of fixation, processing and staining methods for the central nervous system of vertebrates" . Biocell 20, no. 3 (1996) : 265-272.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v20_n3_p265_AldanaMarcos [ ]
---------- MLA ----------
Aldana Marcos, H.J., Ferrari, C.C., Benitez, I., Affanni, J.M. "Standardization of fixation, processing and staining methods for the central nervous system of vertebrates" . Biocell, vol. 20, no. 3, 1996, pp. 265-272.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v20_n3_p265_AldanaMarcos [ ]
---------- VANCOUVER ----------
Aldana Marcos, H.J., Ferrari, C.C., Benitez, I., Affanni, J.M. Standardization of fixation, processing and staining methods for the central nervous system of vertebrates. Biocell. 1996;20(3):265-272.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v20_n3_p265_AldanaMarcos [ ]
