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Abstract:

Fucosylated glycoconjugates play an important role in fertilization as the recognition signal of the zona pellucida. In this work, using "critical" concentrations of either, FITC Lotus tetragonolobus lectin or FITC α-L-fucosyl-BSA neoglycoprotein as molecular probes, population densities of fucosylated glycoconjugates and of their "complementary" molecules (carrying fucosyl receptors), were found all over the sperm surface with higher population densities in post acrosomal sheath, neck and midpiece. These results were compared with previously reported data on the population densities of lactosaminic compounds and their "complementary" molecules, obtained on same samples of spermatozoa. Statistical data demonstrate that fucosylated glycoconjugates share the same domains with biantennary N-acetyllactosaminic oligosaccharides carrying outer galactose and bisected N-acetylglucosamine residues. These domains highly differ with those of the lactosaminic glycoproteins carrying tri and tetraantennary N-acetyllactosaminic oligosaccharides. These studies also show that the domains of fucosylated glycoconjugates and their "complementary" molecules (carrying fucosyl receptors) locate in different zones of the spermatozoon than those of the compounds carrying β-galactosyl receptors. Besides, the results suggest structural differences between fucosylated glycoconjugates of the acrosome, equatorial zone and post acrosomal sheath. This may be relevant to the different biological behavior of these compounds and zones, in fertilization.

Registro:

Documento: Artículo
Título:Fucosylated glycoconjugates of the human spermatozoon. Comparison of the domains of these glycoconjugates with the α-fucosyl binding sites, and with lactosaminic glycoconjugates and β-D-galactosyl binding site domains
Autor:De Cerezo, J.M.S.; Marquínez, A.C.; Sarchi, M.I.; Cerezo, A.S.
Filiación:Ctro. de Invest. en Reprod., Facultad de Medicina (UBA), Paraguay 2155, CP: 1121, Buenos Aires, Argentina
Depto. de Quim. Orgánica, Fac. de Ciencias Exactas y Naturales, Ciudad Universitaria, CP: 1428, Buenos Aires, Argentina
Depto. de Físico Matemat., Fac. de Farmacia y Bioquímica, Junín 956, CP: 1113, Buenos Aires, Argentina
Palabras clave:Fucose; Glycoconjugates; Human spermatozoon; aminosugar; fluorescein isothiocyanate; follitropin; fucose; galactose; glycoconjugate; lactosamine; lectin; luteinizing hormone; article; binding site; chemistry; comparative study; human; male; metabolism; physiology; protein tertiary structure; spermatozoon; ultrastructure; Amino Sugars; Binding Sites; Fluorescein-5-isothiocyanate; Follicle Stimulating Hormone; Fucose; Galactose; Glycoconjugates; Humans; Lectins; Luteinizing Hormone; Male; Protein Structure, Tertiary; Spermatozoa
Año:1996
Volumen:20
Número:1
Página de inicio:11
Página de fin:20
Título revista:Biocell
Título revista abreviado:Biocell
ISSN:03279545
CODEN:BOCEE
CAS:Amino Sugars; Fluorescein-5-isothiocyanate, 3326-32-7; Follicle Stimulating Hormone, 9002-68-0; Fucose, 3713-31-3; Galactose, 26566-61-0; Glycoconjugates; lactosamine, 13000-25-4; Lectins; Luteinizing Hormone, 9002-67-9
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v20_n1_p11_DeCerezo

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Citas:

---------- APA ----------
De Cerezo, J.M.S., Marquínez, A.C., Sarchi, M.I. & Cerezo, A.S. (1996) . Fucosylated glycoconjugates of the human spermatozoon. Comparison of the domains of these glycoconjugates with the α-fucosyl binding sites, and with lactosaminic glycoconjugates and β-D-galactosyl binding site domains. Biocell, 20(1), 11-20.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v20_n1_p11_DeCerezo [ ]
---------- CHICAGO ----------
De Cerezo, J.M.S., Marquínez, A.C., Sarchi, M.I., Cerezo, A.S. "Fucosylated glycoconjugates of the human spermatozoon. Comparison of the domains of these glycoconjugates with the α-fucosyl binding sites, and with lactosaminic glycoconjugates and β-D-galactosyl binding site domains" . Biocell 20, no. 1 (1996) : 11-20.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v20_n1_p11_DeCerezo [ ]
---------- MLA ----------
De Cerezo, J.M.S., Marquínez, A.C., Sarchi, M.I., Cerezo, A.S. "Fucosylated glycoconjugates of the human spermatozoon. Comparison of the domains of these glycoconjugates with the α-fucosyl binding sites, and with lactosaminic glycoconjugates and β-D-galactosyl binding site domains" . Biocell, vol. 20, no. 1, 1996, pp. 11-20.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v20_n1_p11_DeCerezo [ ]
---------- VANCOUVER ----------
De Cerezo, J.M.S., Marquínez, A.C., Sarchi, M.I., Cerezo, A.S. Fucosylated glycoconjugates of the human spermatozoon. Comparison of the domains of these glycoconjugates with the α-fucosyl binding sites, and with lactosaminic glycoconjugates and β-D-galactosyl binding site domains. Biocell. 1996;20(1):11-20.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v20_n1_p11_DeCerezo [ ]