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Abstract:

Metronidazole (MTZ) is a wonderful drug, which is used clinically to treat a wide range of bacterial and protozoal infections. This study aimed to achieve a precise characterization of the cytotoxic and genotoxic activities of MTZ in cultured human lymphocytes at therapeutic concentrations and to evaluate the possible cell death mechanism associated with it. A significant decrease in Mitotic Index (P < 0.001) as well as an increase in Sister Chromatid Exchange (P < 0.001) and Chromosomal Aberrations (P < 0.001) frequencies with no modifications in Replication Index was observed. DNA extracts of MTZ treated cells resulted in nucleosomal DNA ladder pattern after 48 h of cell treatment and this pattern correlated with a decrease in cellular viability (P < 0.001), morphological evidence of apoptosis and increase in the percentage of nuclei with hypodiploid DNA content (P < 0.001). We concluded that MTZ is genotoxic, cytotoxic and is able to modulate cell death through apoptotic mechanisms in the experimental design employed.

Registro:

Documento: Artículo
Título:Use of short term genotoxicological tests to study cytotoxic, genotoxic and cell death mechanism of Metronidazole
Autor:López Nigro, M.M.; Mudry, M.D.; Carballo, M.A.
Filiación:CIGETOX (Citogenética Humana y Genética Toxicológica), INFIBIOC (Instituto de Fisiolopatología y Bioquímica Clínica), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956 (C1113AAD), Ciudad Autónoma de Buenos Aires, Argentina
GIBE-Grupo de Investigación en Biología Evolutiva, Dpto. Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pab. II (C1428EHA), Ciudad Autónoma de Buenos Aires, Argentina
Palabras clave:Cell death; Cytotoxicity; Genetic toxicology; Genotoxicity; Metronidazole; Short term tests; metronidazole; adult; apoptosis; article; cell death; cell viability; chromosome aberration; chromosome replication; concentration response; controlled study; diploidy; DNA content; DNA structure; drug cytotoxicity; female; genotoxicity; human; human cell; lymphocyte; male; mitosis inhibition; sister chromatid exchange; toxicity testing
Año:2010
Volumen:29
Número:8
Página de inicio:1319
Página de fin:1327
Título revista:Latin American Journal of Pharmacy
Título revista abreviado:Lat. Am. J. Pharm.
ISSN:03262383
CAS:metronidazole, 39322-38-8, 443-48-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03262383_v29_n8_p1319_LopezNigro

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Citas:

---------- APA ----------
López Nigro, M.M., Mudry, M.D. & Carballo, M.A. (2010) . Use of short term genotoxicological tests to study cytotoxic, genotoxic and cell death mechanism of Metronidazole. Latin American Journal of Pharmacy, 29(8), 1319-1327.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03262383_v29_n8_p1319_LopezNigro [ ]
---------- CHICAGO ----------
López Nigro, M.M., Mudry, M.D., Carballo, M.A. "Use of short term genotoxicological tests to study cytotoxic, genotoxic and cell death mechanism of Metronidazole" . Latin American Journal of Pharmacy 29, no. 8 (2010) : 1319-1327.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03262383_v29_n8_p1319_LopezNigro [ ]
---------- MLA ----------
López Nigro, M.M., Mudry, M.D., Carballo, M.A. "Use of short term genotoxicological tests to study cytotoxic, genotoxic and cell death mechanism of Metronidazole" . Latin American Journal of Pharmacy, vol. 29, no. 8, 2010, pp. 1319-1327.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03262383_v29_n8_p1319_LopezNigro [ ]
---------- VANCOUVER ----------
López Nigro, M.M., Mudry, M.D., Carballo, M.A. Use of short term genotoxicological tests to study cytotoxic, genotoxic and cell death mechanism of Metronidazole. Lat. Am. J. Pharm. 2010;29(8):1319-1327.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03262383_v29_n8_p1319_LopezNigro [ ]