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Abstract:

Leptospirosis is a worldwide zoonosis caused by a spirochete that belongs to the genus Leptospira. In the last years, new methods, such as the PCR-based multiple-locus variable-number tandem repeat analysis (MLVA), have been developed for the genotyping of leptospires. In the present work, the MLVA patterns for all reference strains used in Argentina for bovine, ovine, porcine, equine, caprine and canine leptospirosis diagnosis, as well as in human and wild animal diagnosis, were obtained. MLVA results are presented in such a way that they can be readily used for the identification of these strains by the simple and direct comparison of agarose gels. Making the use and interpretation of the MLVA for leptospires typing easier will help increase the use of this method as a routine procedure for human and animal diagnosis, for epidemiological studies, vaccine control and other applications.

Registro:

Documento: Artículo
Título:Multiple-locus variable-number tandem repeat analysis of reference strains used for the diagnosis of leptospirosis in Argentina
Autor:Pavan, M.E.; Brihuega, B.; Pettinari, M.J.; Cairó, F.
Filiación:Biochemiq S.A, Laboratorio de Biología Molecular, Ciudad Autónoma de Buenos Aires, Argentina
World Organisation for Animal Health (OIE) Leptospirosis Reference Laboratory, Inst. de Patobiologia Centro de Investigaciones en Ciencias Veterinarias y Agronomicas (CICVyA), Instituto Nacional de Tecnología Agropecuaria (INTA), Hurlingham, Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina
Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina
Facultad de Ciencias Veterinarias, Universidad del Salvador, Pilar, Buenos Aires, Argentina
Palabras clave:Genotyping; Leptospira borgpetersenii; Leptospira interrogans; Leptospira kirschneri; MLVA; Reference strains; VNTR; agar gel electrophoresis; Argentina; article; bacterial strain; bacterium identification; genotype; intermethod comparison; Leptospira borgpetersenii; Leptospira interrogans; Leptospira kirschneri; leptospirosis; multiple locus variable number tandem repeat analysis; nonhuman; variable number of tandem repeat; Animals; Animals, Domestic; Animals, Wild; Argentina; DNA, Bacterial; Electrophoresis, Agar Gel; Genotype; Humans; Leptospira; Leptospira interrogans; Leptospirosis; Minisatellite Repeats; Reference Standards; Species Specificity
Año:2011
Volumen:43
Número:4
Página de inicio:251
Página de fin:255
Handle:http://hdl.handle.net/20.500.12110/paper_03257541_v43_n4_p251_Pavan
Título revista:Revista Argentina de Microbiologia
Título revista abreviado:Rev. Argent. Microbiol.
ISSN:03257541
CODEN:RAMID
CAS:DNA, Bacterial
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_03257541_v43_n4_p251_Pavan.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v43_n4_p251_Pavan

Referencias:

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Citas:

---------- APA ----------
Pavan, M.E., Brihuega, B., Pettinari, M.J. & Cairó, F. (2011) . Multiple-locus variable-number tandem repeat analysis of reference strains used for the diagnosis of leptospirosis in Argentina . Revista Argentina de Microbiologia, 43(4), 251-255.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v43_n4_p251_Pavan [ ]
---------- CHICAGO ----------
Pavan, M.E., Brihuega, B., Pettinari, M.J., Cairó, F. "Multiple-locus variable-number tandem repeat analysis of reference strains used for the diagnosis of leptospirosis in Argentina " . Revista Argentina de Microbiologia 43, no. 4 (2011) : 251-255.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v43_n4_p251_Pavan [ ]
---------- MLA ----------
Pavan, M.E., Brihuega, B., Pettinari, M.J., Cairó, F. "Multiple-locus variable-number tandem repeat analysis of reference strains used for the diagnosis of leptospirosis in Argentina " . Revista Argentina de Microbiologia, vol. 43, no. 4, 2011, pp. 251-255.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v43_n4_p251_Pavan [ ]
---------- VANCOUVER ----------
Pavan, M.E., Brihuega, B., Pettinari, M.J., Cairó, F. Multiple-locus variable-number tandem repeat analysis of reference strains used for the diagnosis of leptospirosis in Argentina . Rev. Argent. Microbiol. 2011;43(4):251-255.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v43_n4_p251_Pavan [ ]