Abstract:
Caseous lymphadenitis (CLA) is a chronic bacterial, infectious and contagious disease caused by Corynebacterium pseudotuberculosis. It affects sheep and results in abscesses of the lymph nodes in subcutaneous tissue, as well as in internal organs such as lungs, liver and kidneys. Differential diagnosis of the disease is based on the isolation and biochemical identification of the etiological agent. The purpose of this study was to characterize the bacteria isolated from typical CLA lesions in sheep from Patagonia, Argentina, at metabolic and genetic levels. Macroscopic observations show a fibrous membrane containing caseous necrotic tissue. Histopathological analysis shows an eosinophilic necrotic area surrounded by epitheloid cells and polymorphonuclear infiltration. Other analyses performed such as microscopic observations, in vitro culture, biochemical tests and 16s rDNA sequencing confirmed diagnosis of caseous lymphadenitis due to C. pseudotuberculosis.
Registro:
Documento: |
Artículo
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Título: | Diagnosis of caseous lymphadenitis in sheep from Patagonia |
Autor: | Estevao Belchior, S.; Gallardo, A.; Ábalos, A.; Díaz, Y.; Álvarez, L.; Callejo, R.; Prieto, M.; Jodor, N.; Jensen, O. |
Filiación: | Centro Regional de Investigación Y Desarrollo Científico Tecnológico (CRIDECIT), Facultad de Ciencias Naturales, Ciudad Universitaria, Km 4, (9000) Comodoro Rivadavia, Chubut, Argentina Servicio de Bacteriología Especial, INEI-ANLIS Dr. Carlos G. Malbrán, Avda. Vélez Sarsfield 563, (1281) Cd. Auton. de Buenos Aires, Argentina Departamento de Zoonosis, Secretaría de Salud de la Provincia de Chubut, Chacra No 18, (9020) Sarmiento, Chubut, Argentina
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Palabras clave: | Corynebacterium pseudotuberculosis; Pseudotuberculosis; Sheep; DNA 16S; Argentina; article; bacterium identification; bacterium isolation; cell infiltration; diagnostic procedure; DNA sequence; epithelioid cell; histopathology; lymphadenitis; nonhuman; polymorphonuclear cell; sheep; tissue necrosis; Animals; Argentina; Corynebacterium Infections; Corynebacterium pseudotuberculosis; Sheep Diseases |
Año: | 2007
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Volumen: | 39
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Número: | 1
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Página de inicio: | 44
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Página de fin: | 46
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Título revista: | Revista Argentina de Microbiologia
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Título revista abreviado: | Rev. Argent. Microbiol.
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ISSN: | 03257541
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CODEN: | RAMID
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v39_n1_p44_EstevaoBelchior |
Referencias:
- Aleman, M., Spier, S.J., Corynebacterium pseudotuberculosis infection (2001) Large Animal Internal Medicine, pp. 1078-1084. , Smith PB, editor, St Louis, Mosby Co
- Brown, C.C., Olander, H.J., Caseous lymphadenitis of goats and sheep: A review (1987) Veter Bull, 57, pp. 1-12
- Estevao Belchior, S., Gallardo, A., Ábalos, A., Jodor, N., Jensen, O., Actualización sobre linfoadenitis caseosa: El agente etiológico y la enfermedad (2006) Rev Vet Argent, 23, pp. 258-278
- Isenberg HD. Clinical Microbiology Procedures Handbook. ASM Press, Washington D.C., 1992, p. 1.20.28-39; 5.1.1-29; Judson, R., Songer, J.G., Corynebacterium pseudotuberculosis: In vitro susceptibility to 39 antimicrobial agents (1991) Vet Microbiol, 27, pp. 145-150
- Leardini, N., Prieto, M., Martínez, C., Aguerre, L., Loaysa, R., Infecciones por bacilos gram-positivos aeróbicos, corine-bacterias (2002) Bacillus, , y Actinomycetes. Curso ANLIS
- León-Viscaino, L., Garrido Abellán, F., González Candela, M., Cubera Pablo, M.J., Clínica de la pseudotuberculosis, , http/www.exopol.com/general/circulares/192.html, Disponible en
- Literák, I., Horváthová, A., Jahnová, M., Rychlk, I., Skalka, B., Phenotype and genotype characteristics of the Slovak and Czech Corynebacterium pseudotuberculosis strains isolated from sheep and goats (1999) Small Ruminant Res, 32, pp. 107-111
- Lipsky, B.A., Goldberger, A.C., Tompkins, L.S., Plorde, J.J., Infections caused by nondiphtheria corynebacteria (1982) Rev Infect Dis, 4, pp. 1220-1235
- National Committee for Clinical Laboratory Standards (NCCLS). Zone diameter interpretative standards and equivalent minimum inhibitory concentration (MIC) breakpoints for Staphylococcus spp. NCCLS 2004; M2-A6. Villanova, Pa, USA; Paton, M.W., Rose, I.R., Hart, R.A., Sutherland, S.S., Mercy, A.R., Ellis, T.M., New infection with Corynebacterium pseudotuberculosis reduces wool production (1994) Aust Vet J, 71, pp. 47-49
- Pépin, M., Boisramé, A., Marly, J., Corynebacterium pseudotuberculosis: Biochemical properties, production of toxin and virulence of ovine and caprine strains (1989) Ann Rech Vet, 20, pp. 111-115
- Songer, J.G., Beckenbach, K., Marshall, M.M., Olson, G.B., Kelly, L., Biochemical and genetic characterization of Corynebacterium pseudotuberculosis (1988) Am J Vet Res, 49, pp. 223-226
- Songer, J.G., Bacterial phospholipases and their role in virulence (1997) Trends Microbiol, 5, pp. 156-161
- Von Graevenitz, A., Krech, T., The genus Corynebacterium (1992) The Prokaryotes: A Handbook on the Biology of Bacteria - Ecophysiology, Isolation, Identification, Applications, pp. 1172-1187. , Balows A, Trüper HG, Dworkin M, Harder W, Schleifer KH, editors, Berlin, Springler-Verlag
Citas:
---------- APA ----------
Estevao Belchior, S., Gallardo, A., Ábalos, A., Díaz, Y., Álvarez, L., Callejo, R., Prieto, M.,..., Jensen, O.
(2007)
. Diagnosis of caseous lymphadenitis in sheep from Patagonia . Revista Argentina de Microbiologia, 39(1), 44-46.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v39_n1_p44_EstevaoBelchior [ ]
---------- CHICAGO ----------
Estevao Belchior, S., Gallardo, A., Ábalos, A., Díaz, Y., Álvarez, L., Callejo, R., et al.
"Diagnosis of caseous lymphadenitis in sheep from Patagonia "
. Revista Argentina de Microbiologia 39, no. 1
(2007) : 44-46.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v39_n1_p44_EstevaoBelchior [ ]
---------- MLA ----------
Estevao Belchior, S., Gallardo, A., Ábalos, A., Díaz, Y., Álvarez, L., Callejo, R., et al.
"Diagnosis of caseous lymphadenitis in sheep from Patagonia "
. Revista Argentina de Microbiologia, vol. 39, no. 1, 2007, pp. 44-46.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v39_n1_p44_EstevaoBelchior [ ]
---------- VANCOUVER ----------
Estevao Belchior, S., Gallardo, A., Ábalos, A., Díaz, Y., Álvarez, L., Callejo, R., et al. Diagnosis of caseous lymphadenitis in sheep from Patagonia . Rev. Argent. Microbiol. 2007;39(1):44-46.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v39_n1_p44_EstevaoBelchior [ ]