Artículo
Santi, L.; Cerrutti, P.; Pilosof, A.M.R.; De Huergo, M.S. "Optimization of electroporation conditions along with nisin treatment for Pseudomonas aeruginosa inhibition " (2003) Revista Argentina de Microbiologia. 35(4):198-204
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Abstract:
A mathematical approach was applied in order to optimize the effect of electroporation by application of pulsed electric fields (PEF) and nisin addition on the inhibition of a strain of Pseudomonas aeruginosa isolated from river sediments. This strain showed to be highly resistant to nisin as only two log cycles reduction of viable cells were obtained in the presence of 84,000 IU/ml nisin. But when a combination of bacteriocin and selected PEF treatment conditions were applied, 4.4-decimal log cycle reduction could be achieved. PEF and nisin interaction seems to be complex, as at lower electric field intensities (i.e., 5 kV/cm) an increment in the number of pulses applied clearly induced a lower inhibitory effect of nisin. At higher PEF intensities (i.e., 11 kV/cm), the inhibitory effect of nisin increased with the number of pulses applied. Results overall, the obtained indicate the possibility of combining PEF and nisin treatments in order to improve the inhibition of resistant microorganisms. The Doehlert experimental design and surface response methodology was an interesting tool to obtain or predict the optimal combination of the stress factors applied.
Registro:
| Documento: | Artículo |
| Título: | Optimization of electroporation conditions along with nisin treatment for Pseudomonas aeruginosa inhibition |
| Autor: | Santi, L.; Cerrutti, P.; Pilosof, A.M.R.; De Huergo, M.S. |
| Filiación: | Facultad de Ingeniería, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina |
| Palabras clave: | Electroporation; Mathematical model; Nisin; Pseudomonas aeruginosa; bacteriocin; nisin; article; bacterial growth; bacterium isolate; electric field; electroporation; growth inhibition; mathematical analysis; mathematical model; nonhuman; Pseudomonas aeruginosa; river ecosystem; sediment; Dose-Response Relationship, Drug; Electromagnetic Fields; Electrophoresis, Gel, Pulsed-Field; Electroporation; Microbial Sensitivity Tests; Models, Theoretical; Nisin; Pseudomonas aeruginosa; Regression Analysis; Water Microbiology |
| Año: | 2003 |
| Volumen: | 35 |
| Número: | 4 |
| Página de inicio: | 198 |
| Página de fin: | 204 |
| Título revista: | Revista Argentina de Microbiologia |
| Título revista abreviado: | Rev. Argent. Microbiol. |
| ISSN: | 03257541 |
| CODEN: | RAMID |
| CAS: | bacteriocin, 11047-01-1; nisin, 1414-45-5; Nisin, 1414-45-5 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v35_n4_p198_Santi |
Referencias:
- Barbosa-Cánovas, G., Pothakamury, V., Swanson, B., Biological effects and applications of pulsed electric fields. Biological aspects (1997) Nonthermal Preservation of Foods, pp. 73-95. , Gould G W (Ed). Blackie Academic & Professional Chapman and Hall, New York
- Barsotti, L., Cheftel, J.C., Food processing by pulsed electric fields. II. Biological aspects (1999) Food Rev. Int., 15, pp. 181-213
- Cerrutti, P., Terebiznik, M.R., De Huergo, M.S., Jagus, R., Pilosof, A.M.R., Combined effect of water activity and pH on the inhibition of Escherichia coli by nisin (2001) J. Food Prot., 64, pp. 1510-1514
- Cutter, C.N., Siragusa, G.R., Incorporation of nisin into a meat binding system to inhibit bacteria on beef surfaces (1998) Lett. Appl. Microbiol., 27, pp. 19-23
- Delves-Broughton, J., The use of EDTA to enhance the efficacy of nisin towards gram-negative bacteria (1993) Int. Biodeter. Biodegrad., 32, pp. 87-97
- Doehlert, D., Uniform shell design (1970) Appl. Stat., 19, pp. 231-239
- Dunn, J., Pulsed electric field processing: An overview (2001) Pulsed Electric Fields in Food Processing, pp. 1-30. , Barbosa-Cánovas G, Zhang Q (Eds). Technomic Publishing Co., Lancaster
- Gould, G.W., Emerging technologies in food preservation and processing in the last 40 years (2000) Innovations in Food Processing, pp. 1-11. , Barbosa-Cánovas G, Gould GW (Eds). Technomic Publishing Co., Lancaster
- Jamieson, L., Williamson, P., The potential of electrotechnologies for the processing of foods (1999) Food Sci. Technol. Today, 13, pp. 97-101
- Jeantet, R., Baron, F., Nau, F., Roignant, M., Brulé, G., High intensity pulsed electric fields applied to egg white: Effect on Salmonella enteritidis inactivation and protein denaturation (1999) J. Food Prot., 62, pp. 1381-1386
- Mazzotta, A., Crandall, A.D., Montville, T.J., Nisin resistance in Clostridium botulinum spores and vegetative cells (1997) Appl. Environ. Microbiol., 63, pp. 2654-2659
- Mertens, B., Knorr, D., Developments of nonthermal processes for food preservation (1992) Food Technol., 5, pp. 125-133
- Peleg, M., A model of microbial survival after exposure to pulsed electric fields (1995) J. Sci. Food Agric., 67, pp. 93-99
- Pol, I.E., Mastwijk, H.C., Bartels, P.V., Smid, E.J., Pulsed-electric field treatment enhances the bactericidal action of nisin against Bacillus cereus (2000) Appl. Environ. Microbiol., 66, pp. 428-430
- Rollema, H., Kuipers, O., Both, P., De Vos, W.M., Siezen, R., Improvement of solubility and stability of the antimicrobial peptide nisin by protein engineering (1995) Appl. Environ. Microbiol., 61, pp. 2873-2878
- Roberts, T.A., Microbial growth and survival: Developments in predictive modeling (1997) Food Technol., 51, pp. 88-90
- Schaffner, D.W., Labuza, T.P., Predictive microbiology: Where are we, and where are we going? (1997) Food Technol., 51, pp. 95-99
- Sitzmann, W., High-voltage pulse techniques for food preservation (1995) New Methods of Food Preservation, pp. 236-252. , Gould GW (Ed). Blackie Academic & Professional- Chapman & Hall, New York
- Soloaga, R., Tokumoto, M., Fernandez, A., Borga, M., Nagel, C., Argüello, E., Gutfraind, Z., Revisión de los mecanismos de resistencia de Pseudomonas aeruginosa a diversas familias de antibiòticos (1996) Infect. Microbiol. Clín., 8, pp. 57-64
- Sorhaug, T., Stepaniak, L., Psychrotrophs and their enzymes in milk and dairy products: Quality aspects (1997) Trends Food Sci. Technol., 8, pp. 35-41
- Stevens, K., Sheldon, B., Klapes, N., Klaenhammer, T., Nisin treatment for the inactivation of Salmonella species and other gram negative bacteria (1991) Appl. Environ. Microbiol., 57, pp. 3613-3615
- Terebiznik, M.R., Jagus, R.J., Cerrutti, P., De Huergo, M.S., Pilosof, A.M.R., Combined effect of nisin and pulsed electric fields on the inactivation of Escherichia coli (2000) J. Food Prot., 63, pp. 741-746
- Thomas, L.V., Clarkson, M.R., Delves-Broughton, J., Nisin (2000) Natural Food Antimicrobial Systems, pp. 463-524. , A.S. Naidu (Ed). CRC Press, Boca Raton
- Ulmer, H.M., Heinz, V., Ganzle, M.G., Knorr, D., Vogel, R.F., Effects of pulsed electric fields on inactivation and metabolic activity of Lactobacillus plantarum in model beer (2002) J. Appl. Microbiol., 93, pp. 326-335
Citas:
---------- APA ----------
Santi, L., Cerrutti, P., Pilosof, A.M.R. & De Huergo, M.S. (2003) . Optimization of electroporation conditions along with nisin treatment for Pseudomonas aeruginosa inhibition . Revista Argentina de Microbiologia, 35(4), 198-204.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v35_n4_p198_Santi [ ]
---------- CHICAGO ----------
Santi, L., Cerrutti, P., Pilosof, A.M.R., De Huergo, M.S. "Optimization of electroporation conditions along with nisin treatment for Pseudomonas aeruginosa inhibition " . Revista Argentina de Microbiologia 35, no. 4 (2003) : 198-204.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v35_n4_p198_Santi [ ]
---------- MLA ----------
Santi, L., Cerrutti, P., Pilosof, A.M.R., De Huergo, M.S. "Optimization of electroporation conditions along with nisin treatment for Pseudomonas aeruginosa inhibition " . Revista Argentina de Microbiologia, vol. 35, no. 4, 2003, pp. 198-204.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v35_n4_p198_Santi [ ]
---------- VANCOUVER ----------
Santi, L., Cerrutti, P., Pilosof, A.M.R., De Huergo, M.S. Optimization of electroporation conditions along with nisin treatment for Pseudomonas aeruginosa inhibition . Rev. Argent. Microbiol. 2003;35(4):198-204.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v35_n4_p198_Santi [ ]
