Artículo
Abstract:
The inhibitory activity of caffeine, a pharmacologically active compound, on Junin virus (JV) multiplication in Veto cells was evaluated by a virus yield inhibition assay. The compound achieved a dose-dependent inhibition of virus production at concentrations not affecting cell viability. The 50% inhibitory concentration (IC50) and the 90% inhibitory concentration (IC90) were 9.0 mM and 10.8 mM, respectively. From time of addition experiments, it can be concluded that caffeine inhibited an early stage in the replicative cycle of JV occurring before 4 h of infection. Extracellular and cell-associated virus yields were reduced to the same extent. The addition of caffeine after several cycles of infection for a very short treatment period did not significantly affect the formation of JV infectious particles. The expression of viral proteins in the cytoplasm and the membrane of infected cells was highly reduced in the presence of caffeine, as revealed by immunofluorescence staining, confirming that caffeine predominantly exerted its inhibitory action early in the infection of Vero cells with JV.
Registro:
| Documento: | Artículo |
| Título: | Inhibition of Junin virus multiplication by caffeine |
| Autor: | Candurra, N.A.; Damonte, E.B. |
| Filiación: | Laboratorio de Virologia, Departamento de Quimica Biologica, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina |
| Palabras clave: | Antiviral activity; Arenaviruses; Argentine hemorrhagic fever; Caffeine; Junin virus; caffeine; virus protein; antivirus agent; caffeine; animal cell; antiviral activity; article; dose response; immunofluorescence; junin virus; nonhuman; protein expression; vero cell; virogenesis; animal; biosynthesis; Cercopithecus; drug effect; fluorescent antibody technique; Junin virus; physiology; time; Vero cell; virus replication; Animals; Antiviral Agents; Caffeine; Cercopithecus aethiops; Dose-Response Relationship, Drug; Fluorescent Antibody Technique, Indirect; Junin virus; Time Factors; Vero Cells; Viral Proteins; Virus Replication |
| Año: | 1999 |
| Volumen: | 31 |
| Número: | 3 |
| Página de inicio: | 135 |
| Página de fin: | 141 |
| Título revista: | Revista Argentina de Microbiologia |
| Título revista abreviado: | Rev. Argent. Microbiol. |
| ISSN: | 03257541 |
| CODEN: | RAMID |
| CAS: | caffeine, 30388-07-9, 58-08-2; Antiviral Agents; Caffeine, 58-08-2; Viral Proteins |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v31_n3_p135_Candurra |
Citas:
---------- APA ----------
Candurra, N.A. & Damonte, E.B. (1999) . Inhibition of Junin virus multiplication by caffeine . Revista Argentina de Microbiologia, 31(3), 135-141.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v31_n3_p135_Candurra [ ]
---------- CHICAGO ----------
Candurra, N.A., Damonte, E.B. "Inhibition of Junin virus multiplication by caffeine " . Revista Argentina de Microbiologia 31, no. 3 (1999) : 135-141.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v31_n3_p135_Candurra [ ]
---------- MLA ----------
Candurra, N.A., Damonte, E.B. "Inhibition of Junin virus multiplication by caffeine " . Revista Argentina de Microbiologia, vol. 31, no. 3, 1999, pp. 135-141.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v31_n3_p135_Candurra [ ]
---------- VANCOUVER ----------
Candurra, N.A., Damonte, E.B. Inhibition of Junin virus multiplication by caffeine . Rev. Argent. Microbiol. 1999;31(3):135-141.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v31_n3_p135_Candurra [ ]
