Artículo
Abstract:
The influence of temperature and pH on the activity and stability of the cellulase system (endoglucanase, exoglucanase and cellobiase) was investigated in Nectria catalinensis. Optimal temperature for the activity of the cellulase system ranged from 50 to 55 °C, with an optimum for stability between 23 °C and 37 °C after a 72 h incubation period. For the different enzymes, maximal activity was registered between pH 4.2-5,8, with pH 4.8 being close to optimal for all stability studies. The activation energy was 4.97 Kcal mol-1 for endoglucanase, 4.37 Kcal mol-1 for exoglucanase and 13.73 Kcal mol-1 for cellobiase. The K(m) and V(max) values were 1.73 mg CMC ml-1 and 0.45 μmol glucose min-1 mg protein-1 for endoglucanase, 0.22 mg microcrystalline cellulose ml-1 and 57.1 nmol glucose min-1 mg protein-1 for exoglucanase, and 2.95 mM cellobiose and 0.17 μmol glucose min-1 mg protein-1 for cellobiase.
Registro:
| Documento: | Artículo |
| Título: | Influence of temperature and pH on cellulase activity and stability in Nectria catalinensis |
| Autor: | Pardo, A.G.; Forchiassin, F. |
| Filiación: | Lab. de Micologia Experimental, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina |
| Palabras clave: | Cellulases; Fungi; Nechia catalinensis; beta glucosidase; cellulase; exoglucanase; glucan synthase; glucose; unclassified drug; article; ascomycetes; enzyme activity; enzyme kinetics; enzyme stability; nectria catalinensis; nonhuman; pH measurement; temperature sensitivity; Cellulase; Enzyme Stability; Hydrogen-Ion Concentration; Hypocreales; Temperature |
| Año: | 1999 |
| Volumen: | 31 |
| Número: | 1 |
| Página de inicio: | 31 |
| Página de fin: | 35 |
| Título revista: | Revista Argentina de Microbiologia |
| Título revista abreviado: | Rev. Argent. Microbiol. |
| ISSN: | 03257541 |
| CODEN: | RAMID |
| CAS: | Cellulase, EC 3.2.1.4 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v31_n1_p31_Pardo |
Citas:
---------- APA ----------
Pardo, A.G. & Forchiassin, F. (1999) . Influence of temperature and pH on cellulase activity and stability in Nectria catalinensis. Revista Argentina de Microbiologia, 31(1), 31-35.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v31_n1_p31_Pardo [ ]
---------- CHICAGO ----------
Pardo, A.G., Forchiassin, F. "Influence of temperature and pH on cellulase activity and stability in Nectria catalinensis" . Revista Argentina de Microbiologia 31, no. 1 (1999) : 31-35.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v31_n1_p31_Pardo [ ]
---------- MLA ----------
Pardo, A.G., Forchiassin, F. "Influence of temperature and pH on cellulase activity and stability in Nectria catalinensis" . Revista Argentina de Microbiologia, vol. 31, no. 1, 1999, pp. 31-35.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v31_n1_p31_Pardo [ ]
---------- VANCOUVER ----------
Pardo, A.G., Forchiassin, F. Influence of temperature and pH on cellulase activity and stability in Nectria catalinensis. Rev. Argent. Microbiol. 1999;31(1):31-35.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v31_n1_p31_Pardo [ ]
