Bacillus megaterium accumulates poly-(3-hydroxybutyrate) (PHB) as a reserve material in intracellular granules. In this work we described a method for the preparation of PHB granules from B. megaterium PV447 and the analysis by polyacrylamide gel electrophoresis of the associated proteins. By comparison with another species a function is proposed for some of these proteins.
Documento: | Artículo |
Título: | Poly-(3-hydroxybutyrate) granules in Bacillus megaterium. Isolation and analysis of associated proteins |
Autor: | Vázquez, G.J.; Wieczoreck, R.; Steinbüchel, A.; Méndez, B.S. |
Filiación: | Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Bueńos Aires, Argentina |
Palabras clave: | acyltransferase; bacterial protein; hydroxybutyric acid; lectin; phasin; plant lectin; poly(3 hydroxybutyric acid); poly(3-hydroxyalkanoic acid) synthase; poly-beta-hydroxybutyrate; polyester; polyhydroxyalkanoate synthase; article; Bacillus megaterium; cell fractionation; cell granule; chemistry; Chromatium; comparative study; enzymology; isolation and purification; polyacrylamide gel electrophoresis; species difference; ultrastructure; Acyltransferases; Bacillus megaterium; Bacterial Proteins; Cell Fractionation; Chromatium; Cytoplasmic Granules; Electrophoresis, Polyacrylamide Gel; Hydroxybutyrates; Lectins; Plant Lectins; Polyesters; Species Specificity |
Año: | 1996 |
Volumen: | 28 |
Número: | 3 |
Página de inicio: | 118 |
Página de fin: | 122 |
Título revista: | Revista Argentina de microbiología |
Título revista abreviado: | Rev. Argent. Microbiol. |
ISSN: | 03257541 |
CAS: | acyltransferase, 9012-30-0, 9054-54-0; hydroxybutyric acid, 1320-61-2, 35054-79-6; poly(3 hydroxybutyric acid), 26063-00-3; Acyltransferases, EC 2.3.-; Bacterial Proteins; Hydroxybutyrates; Lectins; phasin, 1392-87-6, EC 2.3.-; Plant Lectins; poly(3-hydroxyalkanoic acid) synthase, EC 2.3.1.-; poly-beta-hydroxybutyrate, 26063-00-3; Polyesters |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v28_n3_p118_Vazquez |