Endoglucanase production was measured in culture filtrates of four species of Saccobolus growing in media containing glucose or crystalline cellulose as the only carbon sources. Enzyme activity was four to seven times higher in the presence of cellulose than glucose. S. saccoboloides showed maximal growth and enzyme production. The extracellular proteins secreted during growth on cellulose were separated by polyacrylamide gel electrophoresis and stained for proteins. A zymogram technique was used to visualize bands with endoglucanase activity. The four species showed different protein and isoenzyme patterns.
Documento: | Artículo |
Título: | Endoglucanase production in 4 species of the genus Saccobolus |
Autor: | Ramos, A.M.; Forchiassin, F. |
Filiación: | Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina |
Palabras clave: | cellulase; cellulose; fungal protein; glucose; isoenzyme; article; Ascomycetes; biosynthesis; classification; comparative study; drug effect; enzyme induction; enzymology; gene expression regulation; genetics; metabolism; polyacrylamide gel electrophoresis; species difference; Ascomycota; Cellulase; Cellulose; Electrophoresis, Polyacrylamide Gel; Enzyme Induction; Fungal Proteins; Gene Expression Regulation, Fungal; Glucose; Isoenzymes; Species Specificity |
Año: | 1996 |
Volumen: | 28 |
Número: | 2 |
Página de inicio: | 55 |
Página de fin: | 62 |
Título revista: | Revista Argentina de microbiología |
Título revista abreviado: | Rev. Argent. Microbiol. |
ISSN: | 03257541 |
CAS: | cellulase, 9012-54-8; cellulose, 61991-22-8, 68073-05-2, 9004-34-6; glucose, 50-99-7, 84778-64-3; Cellulase, EC 3.2.1.4; Cellulose, 9004-34-6, 50-99-7; Fungal Proteins; Glucose, 50-99-7; Isoenzymes |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v28_n2_p55_Ramos |