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Abstract:

The effects of different experimental conditions on the antiviral activity of the sulphated xylomannan F6, isolated from the red seaweed Nothogenia fastigiata, against the replication of herpes simplex virus type 1 and 2 (HSV-1 and HSV-2) were studied. The compound was equally effective against several strains of HSV-1 (F, KOS, B-2006 and Field) and HSV-2 (G) in different cell lines (Vero, HEp-2 and BHK-21). Furthermore, the antiviral activity of F6 was independent of the method employed to determine the 50% inhibitory concentration (IC50) (virus yield or plaque reduction tests). The value of the IC50 against HSV-1 was not significantly affected by the initial virus concentration since a variation in the multiplicity of infection from 0.0001 to 0.1 UFP/cell only increased twofold the IC50 from 0.61 to 1.35 micrograms/ml, respectively. The potent inhibitory effect of F6 against high doses of HSV-1 represents a significant advantage for this sulphated polysaccharide with respect to other compounds only active against low viral inocula.

Registro:

Documento: Artículo
Título:Determination of the antiviral activity of a naturally occurring sulfated xylomannan under various experimental conditions
Autor:Pujol, C.A.; Coto, C.E.; Damonte, E.B.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Palabras clave:antivirus agent; mannan; animal; article; cell culture; cell line; cell survival; Cercopithecus; chemistry; drug effect; drug screening; hamster; Herpes simplex virus; human; isolation and purification; kidney; pathology; physiology; seaweed; skin tumor; squamous cell carcinoma; Vero cell; virus culture; virus replication; Animals; Antiviral Agents; Carcinoma, Squamous Cell; Cell Line; Cell Survival; Cercopithecus aethiops; Cricetinae; Drug Evaluation, Preclinical; Humans; Kidney; Mannans; Plaque Assay; Seaweed; Simplexvirus; Skin Neoplasms; Tumor Cells, Cultured; Vero Cells; Virus Replication
Año:1995
Volumen:27
Número:2
Página de inicio:91
Página de fin:98
Título revista:Revista Argentina de microbiología
Título revista abreviado:Rev. Argent. Microbiol.
ISSN:03257541
CAS:mannan, 51395-96-1, 9036-88-8; Antiviral Agents; Mannans
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v27_n2_p91_Pujol

Citas:

---------- APA ----------
Pujol, C.A., Coto, C.E. & Damonte, E.B. (1995) . Determination of the antiviral activity of a naturally occurring sulfated xylomannan under various experimental conditions . Revista Argentina de microbiología, 27(2), 91-98.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v27_n2_p91_Pujol [ ]
---------- CHICAGO ----------
Pujol, C.A., Coto, C.E., Damonte, E.B. "Determination of the antiviral activity of a naturally occurring sulfated xylomannan under various experimental conditions " . Revista Argentina de microbiología 27, no. 2 (1995) : 91-98.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v27_n2_p91_Pujol [ ]
---------- MLA ----------
Pujol, C.A., Coto, C.E., Damonte, E.B. "Determination of the antiviral activity of a naturally occurring sulfated xylomannan under various experimental conditions " . Revista Argentina de microbiología, vol. 27, no. 2, 1995, pp. 91-98.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v27_n2_p91_Pujol [ ]
---------- VANCOUVER ----------
Pujol, C.A., Coto, C.E., Damonte, E.B. Determination of the antiviral activity of a naturally occurring sulfated xylomannan under various experimental conditions . Rev. Argent. Microbiol. 1995;27(2):91-98.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v27_n2_p91_Pujol [ ]