The effects of different experimental conditions on the antiviral activity of the sulphated xylomannan F6, isolated from the red seaweed Nothogenia fastigiata, against the replication of herpes simplex virus type 1 and 2 (HSV-1 and HSV-2) were studied. The compound was equally effective against several strains of HSV-1 (F, KOS, B-2006 and Field) and HSV-2 (G) in different cell lines (Vero, HEp-2 and BHK-21). Furthermore, the antiviral activity of F6 was independent of the method employed to determine the 50% inhibitory concentration (IC50) (virus yield or plaque reduction tests). The value of the IC50 against HSV-1 was not significantly affected by the initial virus concentration since a variation in the multiplicity of infection from 0.0001 to 0.1 UFP/cell only increased twofold the IC50 from 0.61 to 1.35 micrograms/ml, respectively. The potent inhibitory effect of F6 against high doses of HSV-1 represents a significant advantage for this sulphated polysaccharide with respect to other compounds only active against low viral inocula.
Documento: | Artículo |
Título: | Determination of the antiviral activity of a naturally occurring sulfated xylomannan under various experimental conditions |
Autor: | Pujol, C.A.; Coto, C.E.; Damonte, E.B. |
Filiación: | Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina |
Palabras clave: | antivirus agent; mannan; animal; article; cell culture; cell line; cell survival; Cercopithecus; chemistry; drug effect; drug screening; hamster; Herpes simplex virus; human; isolation and purification; kidney; pathology; physiology; seaweed; skin tumor; squamous cell carcinoma; Vero cell; virus culture; virus replication; Animals; Antiviral Agents; Carcinoma, Squamous Cell; Cell Line; Cell Survival; Cercopithecus aethiops; Cricetinae; Drug Evaluation, Preclinical; Humans; Kidney; Mannans; Plaque Assay; Seaweed; Simplexvirus; Skin Neoplasms; Tumor Cells, Cultured; Vero Cells; Virus Replication |
Año: | 1995 |
Volumen: | 27 |
Número: | 2 |
Página de inicio: | 91 |
Página de fin: | 98 |
Título revista: | Revista Argentina de microbiología |
Título revista abreviado: | Rev. Argent. Microbiol. |
ISSN: | 03257541 |
CAS: | mannan, 51395-96-1, 9036-88-8; Antiviral Agents; Mannans |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v27_n2_p91_Pujol |