Kinetics of the humoral immune primary response and seven-day secondary response of adult CF1 mice to FMDV O1 Campos adjuvanted in aluminium hydroxide-saponin (AHS) or in oil emulsion (OE) were evaluated by means of ELISA and passive hemagglutination (PH). Analysis of the response to AHS vaccine showed that ELISA measured maximal titres of primary response at 23 days post-vaccination (dpv), and at day 17 of secondary response, while PH detected maximal titres for primary as well as secondary response around day 60 pv. Mice immunized with OE vaccine studied by ELISA presented a plateau of primary response around 40 dpv, while secondary response was maximal around 80 dpv. The same sera tested by PH showed the highest titres for primary response on day 50 pv and secondary response was maximal on day 40 pv. A criterion for the evaluation of vaccination efficiency in the murine model is proposed based on the methods employed in the determination of antibody level.
| Documento: | Artículo |
| Título: | Comparison of various methods for the measurement of the humoral immune response in mice vaccinated with aphthous fever virus |
| Autor: | Feledi, C.A.; Pujol, C.A.; Arese, A.; Piccone, M.E.; Marcovecchio, F.; Zuloaga, G.; Massouh, E.J. |
| Filiación: | Laboratorio de Inmunoquímica, Facultad de Ciencias Exactas y Naturales, UBA, Argentina |
| Palabras clave: | virus antibody; virus vaccine; animal; Aphthovirus; article; bioassay; biosynthesis; blood; comparative study; enzyme linked immunosorbent assay; evaluation; foot and mouth disease; hemagglutination test; immunology; male; mouse; mouse strain; serodiagnosis; suckling; vaccination; Animals; Animals, Suckling; Antibodies, Viral; Aphthovirus; Biological Assay; Enzyme-Linked Immunosorbent Assay; Evaluation Studies; Foot-and-Mouth Disease; Hemagglutination Tests; Male; Mice; Mice, Inbred Strains; Neutralization Tests; Vaccination; Viral Vaccines |
| Año: | 1992 |
| Volumen: | 24 |
| Número: | 3-4 |
| Página de inicio: | 126 |
| Página de fin: | 135 |
| Título revista: | Revista Argentina de microbiología |
| Título revista abreviado: | Rev. Argent. Microbiol. |
| ISSN: | 03257541 |
| CAS: | Antibodies, Viral; Viral Vaccines |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v24_n3-4_p126_Feledi |
